Volume 3, Issue 3 (2017)                   IEM 2017, 3(3): 100-103 | Back to browse issues page

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Dalimi A, Tahvildar F, Ghaffari far F. Molecular Study on Cryptosporidium andersoni Strains Isolated from Sheep Based on ‎18S rRNA Gene. IEM 2017; 3 (3) :100-103
URL: http://iem.modares.ac.ir/article-4-12226-en.html
1- Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, IR Iran
2- Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, IR Iran Parasitology Department, Medical School, Shahid Beheshti Medical Sciences University, Faculty, Tehran, IR Iran
Abstract:   (3168 Views)
Background: Cryptosporidiosis is one of the most important parasitic diseases infecting a broad variety of animals and humans. In the present study, Nested PCR-RFLP-based assay was applied for genotyping of‏ ‏‏sheep cryptosporidiosis. The target of amplification was the 18S rRNA gene ‎‎used to identify Cryptosporidium species
Materials and Methods: In the first step, 1300 faecal samples were collected from sheep in Tehran province, then the samples were examined for the presence of ‎Cryptosporidium using modified acid fast staining. In the second step, DNA was extracted from the ‎positive samples. Next, 18S rRNA gene was amplified by ‎Nested-PCR in order to differentiate between the species. The PCR product was digested by Ssp1 restriction enzyme. ‎
Results: Twenty two positive ‎sheep samples were detected by modified acid fast method. The results were confirmed by molecular techniques. The 845 bp fragment of 18S rRNA was digested ‎by restriction enzymes. Twenty samples showed ‎a similar band on 2.5% agarose gel whereas 2 samples demonstrated different pattern. The sequences of two patterns indicated two species of C. andersoni and C. parvum.
Conclusion:  In spite of other studies results introducing C. parvum as the major agent of ‎cryptosporidiosis in sheep, in our study, C. andersoni was found to be dominant. 
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Received: 2017/06/3 | Accepted: 2017/07/6 | Published: 2017/09/1

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