Volume 4, Issue 2 (2018)                   IEM 2018, 4(2): 73-77 | Back to browse issues page

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Nikoomanesh F, Roudbar mohammadi‎ S, Bashardoust B, Zareei ‎ M. Effect of Farnesol on Responsive Gene Expressions in Hyphal ‎Morphogenesis Transformation of Candida albicans. IEM. 2018; 4 (2) :73-77
URL: http://journals.modares.ac.ir/article-4-20654-en.html
1- Medical Mycology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Medical Mycology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran , sh.mohammadi@modares.ac.ir
3- Health Department, Rescue & Treatment of I.R. Iran Police Force, Tehran, Iran
Abstract:   (208 Views)
Aims: Candida albicans a polymorphic fungus can grow as yeast, pseudohyphae and true hyphae forms. The hyphal form has a key role in infection process during invasion to mucosal membrane. A cluster of genes contribute in controlling of hyphae formation in C. albicans, include SAP6, HWP1 and RIM101. Farnesol is a quorum sensing molecule which inhibits switching of yeast-to-hyphae form. The aim of this study was to investigate the effect of farnesol on yeast-to-hyphae morphogenesis and its related gene expressions in C. albicans.
Materials and Methods: In this laboratory trial study, C .albicans was exposed to various concentration (5, 10, 20, 50, 100, 150 and 300µM) of farnesol and the rate of yeast cell proliferations and germ tube formation was evaluated by different methods and microscopic examination. Real time-PCR was performed to assess the expression levels of the hyphae-specific genes SAP6, HWP1 and RIM101. The results were analyzed by IBM SPSS 23 software using Student's t-test and one-way ANOVA.
Findings: The yeast growth reduced 5% in 300µM of farnesol approximately (p<0.05). Germ tube formation strongly suppressed. Moreover, Real time-PCR analysis showed that 300µM farnesol decreased HWP1 and SAP6 gene expressions significantly in comparison to control group (p<0.05), whereas, there was no difference in the expression of RIM101 gene.
Conclusion: Farnesol in 300µM concentration can inhibits growth and proliferation of C. albicans yeast cells and also inhibits hyphal formation. Farnesol can affect the expression of virulent genes including pathogenic genes that are associated with hyphae morphogenesis such as SAP6 and HWP1.
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Received: 2018/03/12 | Accepted: 2018/04/29 | Published: 2018/06/20

References
1. Ahmad Khan MS, Ahmad I, Aqil F, Owais M, Shahid M, Musarrat J. Virulence and pathogenicity of fungual ‎pathogens with special reference to Candida albicans. In: Ahmad I, Owais M, Shahid M, Aqil F, editors. ‎Combating fungal infections: Problems and remedy. Berlin: Springer Science & Business Media; 2010. pp. 21-‎‎45.‎ [Link]
2. Sudbery PE. Growth of Candida albicans hyphae. Nat Rev Microbiol. 2011;9(10):737-48.‎ [Link] [DOI:10.1038/nrmicro2636]
3. Finkel JS, Mitchell AP. Genetic control of Candida albicans biofilm development. Nat Rev Microbiol. ‎‎2011;9(2):109-18.‎ [Link] [DOI:10.1038/nrmicro2475]
4. Sundstrom P. Adhesion in Candida spp. Cell Microbiol. 2002;4(8):461-9.‎ [Link] [DOI:10.1046/j.1462-5822.2002.00206.x]
5. Ene IV, Bennett RJ. Hwp1 and related adhesins contribute to both mating and biofilm formation in ‎Candida albicans. Eukaryot Cell. 2009;8(12):1909-13.‎ [Link] [DOI:10.1128/EC.00245-09]
6. Nobile CJ, Solis N, Myers CL, Fay AJ, Deneault JS, Nantel A, et al. Candida albicans transcription factor ‎Rim101 mediates pathogenic interactions through cell wall functions. Cell Mirobiol. 2008;10(11):2180-96.‎ [Link]
7. Biswas S, Van Dijck P, Datta A. Environmental sensing and signal transduction pathways regulating ‎morphopathogenic determinants of Candida albicans. Microbial Mol Biol Rev. 2007;71(2):348-76.‎ [Link] [DOI:10.1128/MMBR.00009-06]
8. Décanis N, Tazi N, Correia A, Vilanova M, Rouabhia M. Farnesol, a fungal quorum-sensing molecule triggers ‎Candida albicans morphological changes by downregulating the expression of different secreted aspartyl ‎proteinase genes. Open Microbiol J. 2011;5:119-26.‎ [Link] [DOI:10.2174/1874285801105010119]
9. Hornby JM, Kebaara BW, Nickerson KW. Farnesol biosynthesis in Candida albicans: Cellular response to ‎sterol inhibition by Zaragozic acid B. Antimicrob Agents Chemother. 2003;47(7):2366-9.‎ [Link] [DOI:10.1128/AAC.47.7.2366-2369.2003]
10. S Derengowski L, De-Souza-Silva C, V Braz S, M Mello-De-Sousa T, N Báo S, M Kyaw C, et al. Antimicrobial ‎effect of farnesol, a Candida albicans quorum sensing molecule, on Paracoccidioides brasiliensis growth and ‎morphogenesis. Ann Clin Microbiol Antimicrob. 2009;8:13.‎ [Link]
11. Bashardoust B, Roudbar Mohammadi Sh, Roudbary M, Nikoomanesh F. Susceptibility evaluation of ‎aspergillus fumigatus to silver nanoparticles compared with voriconazole. Infect Epidemiol Med. ‎‎2016;2(3):20-3.‎ [Link] [DOI:10.18869/modares.iem.2.3.20]
12. Nikoomanesh F, Roudbar Mohammadi Sh, Roudbary M, Bayat M, Heidari Gh. Investigation of bcr1 gene ‎expression in Candida albicans isolates by RT-PCR technique and its impact on biofilm formation. Infect ‎Epidemiol Med. 2016;2(1):22-4.‎ [Link]
13. Roudbary M, Roudbar Mohammadi Sh, Bakhshi B, Farhadi Z, Nikoomanesh F. Identification of Candida ‎species isolated form Iranian women eith vaginal candidasis by PCR-RFLP method. Eur J Exp Biol. ‎‎2013;3(6):365-9.‎ [Link]
14. Jacobsen ID, Wilson D, Wächtler B, Brunke S, Naglik JR, Hube B. Candida albicans dimorphism as a ‎therapeutic target. Expert Rev Anti Infect Ther. 2012;10(1):85-93.‎ [Link]
15. Wächtler B, Wilson D, Haedicke K, Dalle F, Hube B. From attachment to damage: Defined genes of ‎Candida albicans mediate adhesion, invasion and damage during interaction with oral epithelial cells. PLoS ‎One. 2011;6(2):e17046.‎ [Link]
16. Staab JF, Bahn YS, Tai CH, Cook PF, Sundstrom P. Expression of transglutaminase substrate activity on ‎Candida albicans germ tubes through a coiled, disulfide-bonded N-terminal domin of Hwp1 requires C-‎terminal glycosylphosphatidylinositol modification. J Biol Chem. 2004;279(39):40737-47.‎ [Link] [DOI:10.1074/jbc.M406005200]
17. Whiteway M, Bachewich C. Morphogenesis in Candida albicans. Annu Rev Microbiol. 2007;61:529-53.‎ [Link] [DOI:10.1146/annurev.micro.61.080706.093341]
18. Lu Y, Su C, Unoje O, Liu H. Quorum sensing controls hyphal initiation in Candida albicans through Ubr1-‎mediated protein degradation. Proc Natl Acad Sci U S A. 2014;111(5):1975-80.‎ [Link] [DOI:10.1073/pnas.1318690111]
19. Saville SP, Lazzell AL, Bryant AP, Fretzen A, Monreal A, Solberg EO, et al. Inhibition of filamentation can ‎be used to treat disseminated candidiasis. Antimicrob Agents Chemother. 2006;50(10):3312-6.‎ [Link]
20. Semighini CP, Hornby JM, Dumitru R, Nickerson KW, Harris SD. Farnesol-induced apoptosis in Aspergillus ‎nidulans reveals a possible mechanism for antagonistic interactions between fungi. Mol Microbiol. ‎‎2006;59(3):753-64.‎ [Link] [DOI:10.1111/j.1365-2958.2005.04976.x]
21. Ramage G, Saville SP, Wickes BL, López-Ribot JL. Inhibition of Candida albicans biofilm formation by ‎farnesol, a quorum-sensing molecule. Appl Environ Microbiol. 2002;68(11):5459-63.‎ [Link] [DOI:10.1128/AEM.68.11.5459-5463.2002]
22. Mosel DD, Dumitru R, Hornby JM, Atkin AL, Nickerson KW. Farnesol concentrations required to block ‎germ tube formation in Candida albicans in the presence and absence of serum. Appl Environ Microbiol. ‎‎2005;71(8):4938-40.‎ [Link] [DOI:10.1128/AEM.71.8.4938-4940.2005]

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