Volume 4, Issue 4 (2018)                   IEM 2018, 4(4): 0-0 | Back to browse issues page

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Bahroudi S, Nematollahi M A, aghasadeghi mr phd M R, Nazemi M. In vitro evaluation of the antiviral activity and cytotoxicity effect of Holothuria leucospilota sea cucumber extracts from the Persian Gulf. IEM. 2018; 4 (4)
URL: http://journals.modares.ac.ir/article-4-26525-en.html
1- processing marine products, Fisheries Dept. Faculty of Natural Resources/ University of Tehran,Karaj, Iran
2- processing marine products, Fisheries Dept. Faculty of Natural Resources/ University of Tehran,Karaj, Iran , malahi@ut.ac.ir
3- Hepatitis and AIDS Departments, Pasteur Institute of Iran, Tehran, Iran.
4- Persian Gulf and Oman Sea Ecological Research Institute, Bandar abbas, Hormozgan, Iran
Abstract:   (63 Views)
Aims: Antiviral activity and cytotoxicity effect of methanol and diethyl ether extracts from different parts of sea cucumber (Holothuria leucospilota) against HIV-1 were assessed on human oral epidermoid carcinoma cells (KB) and Human embryonic kidney 293T cells (HEK293T).
Methods: Sea cucumber was collected at a depth of 10-30 m of Larak Island (Persian Gulf). Extracts were prepared by diethyl ether and methanol solvents. The antiviral activity of each extract was evaluated by inhibition of Single-cycle replicable HIV-1 (SCR HIV-1) p24 Core antigen production in HeLa cells and cellular toxicity of different extracts were assessed using a cell proliferation XTT kit.
Findings: Antiviral activity of each extract showed that some concentrations were able to inhibit the replication of HIV-1. Diethyl ether extract of body wall with 2.79 TI index displayed the highest antiviral activity as well as less cytotoxicity effect.
Conclusion: This study showed that crude extracts of Holothuria leucospilota, especially methanol and diethyl ether extracts of digestive organs and body wall had good cytotoxicity effect and antiviral activity, respectively.
 
     

Received: 2018/10/27

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