Volume 4, Issue 4 (2018)                   IEM 2018, 4(4): 0-0 | Back to browse issues page

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Shariat bahadori E, Sadraei J, Dalimi A. Evaluation of NT-Pro BNP and Pro Calcitonin as markers from Rodents with myocardial toxoplasmosis using ECL method: A pilot study. IEM. 2018; 4 (4)
URL: http://journals.modares.ac.ir/article-4-27073-en.html
1- Ph.D student of medical Parasitology
2- Ph.D of Medical Parasitology , sadraeij@modares.ac.ir
3- PhD of Medical Parasitology
Abstract:   (87 Views)
Aims:  Toxoplasma parasites that extracted from different rodents are the same in immunologic and morphological characteristics but different in pathogenic characteristics. We found that the serum levels of Pro BNP and Pro Calcitonin markers are high among these rodents. The aim of this study was the assessment of the serum levels of Pro BNP and Pro Calcitonin markers among the rodents with myocardial toxoplasmosis.
Materials and Methods: In this study, we collected 286 rodents and extracted 250g of their heart tissues and blood samples to obtain DNA of T. gondii. We detected the positive samples, using the nested- PCR method. Then, we examined serum levels of Pro BNP and Pro Calcitonin markers, using ECL method (Electro Chemo Luminescence) for assessment of myocardial toxoplasmosis in this host. Data analysis was also conducted by statistical analysis method. This study was performed in January to March 2017, based on the prevalence study.
Findings: In this study 68/286 samples of rodents were positive for GRA6 gene and these positive samples had high levels of Pro BNP and Pro Calcitonin markers that indicated myocardial toxoplasmosis and acute inflammation among these animals.
Conclusions: In this study, we found that the GRA6 gene was very useful to follow up toxoplasmosis in the rodents of the Golestan province, northeast of Iran. Also Pro BNP and Pro Calcitonin markers were at high levels in myocardial toxoplasmosis.
 
     

Received: 2018/11/11

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