Volume 4, Issue 4 (2018)                   IEM 2018, 4(4): 0-0 | Back to browse issues page

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Shivaee A, Mirshekar M, sadeghi kalani B, bordbar D, Ohadi E, masjedian jazi F. evaluation of the effects of curcumin nanoparticles on the expression of genes involved in biofilm formation in UPEC. IEM. 2018; 4 (4)
URL: http://journals.modares.ac.ir/article-4-29809-en.html
1- Department of Microbiology, School of Medicine, Iran University of Medical Sciences,Tehran, Iran
2- Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran
3- Antimicrobial Resistance Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- medical microbiology ,Department of Microbiology, School of Medicine, Iran University of Medical Sciences,Tehran, Iran
5- Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran , fmasjedian@yahoo.com
Abstract:   (69 Views)
Background:  Uropathogenic Escherichia coli (UPEC) is one of the most important causative agent of urinary tract infection (UTI). UPEC isolates persist in the body through biofilm formation. The successful adhesion is the most important step of biofilm formation. Type 1 pili and P fimbriae are bacterial surface appendices which play a pivotal role in adhesion of UPEC. The purpose of this study was to assess the effect of nanocurcumin on the initial adhesion and papG and fimH gene expression in UPEC isolates.
 
 Materials and Methods: The presence of papG and fimH genes among 60 UPEC isolates was investigated by PCR, five potent biofilm producer UPEC strains from patients with urinary tract infection were exposed to the sub-minimum inhibitory concentration of nanocurcumin. Expression of the papG and fimH genes were evaluated by real-time PCR.
 
Result: Of the 60 UPEC isolates, biofilm formation was seen in 27 (45%) of isolates, which 5 isolates produced strong biofilm. The result of PCR assay showed that papG was seen in 57 (95%) of the 60 UPEC isolates and fimH was seen in 58 (96.6%) of isolates respectively. Nanocurcumin decreased papG and fimH expression 7 and 8 fold in all five isolates respectively.
Conclusion: Sub-MIC concentrations of nanocurcumin remarkably decreased the expression of the papG and fimH genes in strong biofilm forming UPEC strains but nanocurcumin cannot prevent biofilm formation
     

Received: 2019/01/26

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