Volume 7, Issue 1 (2021)
IEM 2021, 7(1): 17-28 |
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Ranjbar M M, Enayati S, Lotfi M, Yousefi A R, Azimi S M, Mousavi S R, et al . Simultaneous Detection of Bovine Rotavirus (BRV) and Bovine Viral Diarrhea (BVD) virus in Diarrheic Stool Samples: A Comparative Study of Molecular and Serological approaches. IEM 2021; 7 (1) :17-28
URL: http://iem.modares.ac.ir/article-4-47258-en.html
URL: http://iem.modares.ac.ir/article-4-47258-en.html
Mohammad Mehdi Ranjbar * 
1, Soodeh Enayati2 , Mohsen Lotfi3 , Ali Reza Yousefi3 , Seyed Mahmoud Azimi3 , Seyed Reza Mousavi3 , Sajjad Yazdansetad4 , Gholamreza Karimi3
1, Soodeh Enayati2 , Mohsen Lotfi3 , Ali Reza Yousefi3 , Seyed Mahmoud Azimi3 , Seyed Reza Mousavi3 , Sajjad Yazdansetad4 , Gholamreza Karimi3
1- Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IR Iran , mm.ranjbar.phd@gmail.com
2- Department of Biotechnology Research and Science Branch, Islamic Azad University, Tehran, IR Iran
3- Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IR Iran
4- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran
2- Department of Biotechnology Research and Science Branch, Islamic Azad University, Tehran, IR Iran
3- Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IR Iran
4- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran
Abstract: (1661 Views)
Background: Calf scours (diarrhea) in unweaned calves play a major role in economic losses of animal farming industry worldwide. The present study was conducted to investigate and interpret the presence of BRV, BVDV, and Escherichia coli K99 by molecular and serological approaches simultaneously.
Materials & Methods: A total of 73 E. coli-negative diarrheic fecal samples were collected from one-week to less than one-month-old calves of Holstein dairy cattle herds of some provinces of Iran during autumn and winter. The samples were directed to antigen detection by ELISA (Enzyme Linked ImmunoSorbent Assay), RNA extraction by semi-manual approach, and cDNA synthesis for PCR amplification.
Findings: Out of 73 calves’ diarrheic fecal samples, 28 (38.3%) and 1 (1.36%) were positive for BRV and BVDR by ELISA, respectively. However, 31 (42.4%) samples were positive for BRV and non for BVDV by RT-PCR. The Kappa coefficient showed significant differences in BRV and BVDR detection between ELISA and RT-PCR methods. The distribution of the BRV-positive samples among bovine diarrheic calves was 80, 52.6, and 50% in Eslamshahr, Qazvin, and Hamedan, respectively.
Conclusion: ELISA and RT-PCR indicated high prevalence rate of BRV in autumn and winter, respectively. The present study results showed that positive cases detected by RT-PCR were more than those detected by ELISA. Further studies are needed to achieve a comprehensive preventive and therapeutic strategy to address diarrhea bovine pathogens.
Materials & Methods: A total of 73 E. coli-negative diarrheic fecal samples were collected from one-week to less than one-month-old calves of Holstein dairy cattle herds of some provinces of Iran during autumn and winter. The samples were directed to antigen detection by ELISA (Enzyme Linked ImmunoSorbent Assay), RNA extraction by semi-manual approach, and cDNA synthesis for PCR amplification.
Findings: Out of 73 calves’ diarrheic fecal samples, 28 (38.3%) and 1 (1.36%) were positive for BRV and BVDR by ELISA, respectively. However, 31 (42.4%) samples were positive for BRV and non for BVDV by RT-PCR. The Kappa coefficient showed significant differences in BRV and BVDR detection between ELISA and RT-PCR methods. The distribution of the BRV-positive samples among bovine diarrheic calves was 80, 52.6, and 50% in Eslamshahr, Qazvin, and Hamedan, respectively.
Conclusion: ELISA and RT-PCR indicated high prevalence rate of BRV in autumn and winter, respectively. The present study results showed that positive cases detected by RT-PCR were more than those detected by ELISA. Further studies are needed to achieve a comprehensive preventive and therapeutic strategy to address diarrhea bovine pathogens.
Article Type: Original Research |
Subject:
Virology
Received: 2020/10/31 | Accepted: 2020/12/24 | Published: 2021/01/23
Received: 2020/10/31 | Accepted: 2020/12/24 | Published: 2021/01/23
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