Volume 11, Issue 2 (2025)
IEM 2025, 11(2): 115-121 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Shatizadeh Malekshahi S, Gholami Barzoki M, Shokoofeh M. Exploring Maternal-Fetal Transmission of Human Herpesvirus 6: A Study of Placental, Embryonic, and Autopsy Samples. IEM 2025; 11 (2) :115-121
URL: http://iem.modares.ac.ir/article-4-78342-en.html
URL: http://iem.modares.ac.ir/article-4-78342-en.html
1- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , s.shatizadeh@modares.ac.ir
2- Student Research Committee, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Student Research Committee, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract: (598 Views)
Background: Considering the limited studies conducted on the possibility of vertical transmission of HHV-6 in humans at different stages of pregnancy, the objective of this research wasis to examine the vertical transmission of HHV-6 (human herpesvirus 6) in various tissues of aborted fetuses atduring different months of pregnancy.
Materials & Methods: This research was conducted on used 58 formalin- fixed paraffin- embedded tissues (FFPE) from 26 fetopsies. DNA extraction was performed using the phenol-chloroform technique. The quantity of extracted DNA samples was measured using the NanoDrop spectrophotometric method. PCR of the beta-globin gene confirmed the quality of the extracted DNA, and then the presence of the HHV-6 genome was tested using the Rreal-time PCR method.
Findings: Of the 26 fetuses examined, 22 (84.6%) were negative for HHV-6, and four (15.4%) were positive. All six first-trimester fetuses were negative. Among 13 second-trimester fetuses (29 FFPE tissues), two (7.7%) tested positive., wWhile none of the seven third-trimester fetuses (22 FFPE tissues) had placental positive placentasity. However, HHV-6 was detected in non-placental fetal tissues, including the liver of fetus No. 16 and the heart of fetus No. 22, both of which were in the third trimester.
Conclusion: These findings suggest that while vertical transmission of HHV-6 may occur, particularly in later stages of pregnancy andor in specific fetal tissues, the overall prevalence in theis sample studied was relatively low. Further investigation is needed to understand the implications of these results for maternal and fetal health.
Materials & Methods: This research was conducted on used 58 formalin- fixed paraffin- embedded tissues (FFPE) from 26 fetopsies. DNA extraction was performed using the phenol-chloroform technique. The quantity of extracted DNA samples was measured using the NanoDrop spectrophotometric method. PCR of the beta-globin gene confirmed the quality of the extracted DNA, and then the presence of the HHV-6 genome was tested using the Rreal-time PCR method.
Findings: Of the 26 fetuses examined, 22 (84.6%) were negative for HHV-6, and four (15.4%) were positive. All six first-trimester fetuses were negative. Among 13 second-trimester fetuses (29 FFPE tissues), two (7.7%) tested positive., wWhile none of the seven third-trimester fetuses (22 FFPE tissues) had placental positive placentasity. However, HHV-6 was detected in non-placental fetal tissues, including the liver of fetus No. 16 and the heart of fetus No. 22, both of which were in the third trimester.
Conclusion: These findings suggest that while vertical transmission of HHV-6 may occur, particularly in later stages of pregnancy andor in specific fetal tissues, the overall prevalence in theis sample studied was relatively low. Further investigation is needed to understand the implications of these results for maternal and fetal health.
Article Type: Brief Communication |
Subject:
Virology
Received: 2024/12/5 | Accepted: 2025/01/15 | Published: 2025/04/21
Received: 2024/12/5 | Accepted: 2025/01/15 | Published: 2025/04/21
References
1. Pantry SN, Medveczky PG. Latency, integration, and reactivation of human herpesvirus-6. Viruses. 2017;9(7):194. [DOI:10.3390/v9070194] [PMID] []
2. Arbuckle JH, Medveczky PG. The molecular biology of human herpesvirus-6 latency and telomere integration. Microbes Infect. 2011;13(8-9):731-41. [DOI:10.1016/j.micinf.2011.03.006] [PMID] []
3. Campadelli-Fiume G, Mirandola P, Menotti L. Human herpesvirus 6: An emerging pathogen. Emerg Infect Dis. 1999;5(3):353-66. [DOI:10.3201/eid0503.990306] [PMID] []
4. Agut H, Bonnafous P, Gautheret-Dejean A. Update on infections with human herpesviruses 6A, 6B, and 7. Med Mal Infect. 2017;47(2):83-91. [DOI:10.1016/j.medmal.2016.09.004] [PMID]
5. Komaroff AL, Pellett PE, Jacobson S. Human herpesviruses 6A and 6B in brain diseases: Association versus causation. Clin Microbiol Rev. 2020;34(1):10-128. [DOI:10.1128/CMR.00143-20] [PMID] []
6. Caserta MT, Hall CB, Schnabel K, Lofthus G, McDermott MP. Human herpesvirus (HHV)-6 and HHV-7 infections in pregnant women. J Infect Dis. 2007;196(9):1296-303.
https://doi.org/10.1086/10.1086/522430 [DOI:10.1086/522430] [PMID]
7. Komaroff AL, Rizzo R, Ecker JL. Human herpesviruses 6A and 6B in reproductive diseases. Front Immunol. 2021;12:648945. [DOI:10.3389/fimmu.2021.648945] [PMID] []
8. Braun DK, Dominguez G, Pellett PE. Human herpesvirus 6. Clin Microbiol Rev. 1997;10(3):521-67. [DOI:10.1128/CMR.10.3.521] [PMID] []
9. Levy JA, Ferro F, Greenspan D, Lennette E. Frequent isolation of HHV-6 from saliva and high seroprevalence of the virus in the population. Lancet. 1990;335(8697):1047-50. [DOI:10.1016/0140-6736(90)92628-U] [PMID]
10. Caserta MT, Mock DJ, Dewhurst S. Human herpesvirus 6. Clin Infect Dis. 2001;33(6):829-33. [DOI:10.1086/322691] [PMID]
11. Okuno T, Oishi H, Hayashi K, Nonogaki M, Tanaka K, Yamanishi K. Human herpesviruses 6 and 7 in cervixes of pregnant women. J Clin Microbiol. 1995;33(7):1968-70. [DOI:10.1128/jcm.33.7.1968-1970.1995] [PMID] []
12. Baillargeon J, Piper J, Leach CT. Epidemiology of human herpesvirus 6 (HHV-6) infection in pregnant and nonpregnant women. J Clin Virol. 2000;16(3):149-57. [DOI:10.1016/S1386-6532(99)00086-4] [PMID]
13. Bortolotti D, Soffritti I, D'Accolti M, Gentili V, Di Luca D, Rizzo R, et al. HHV-6A infection of endometrial epithelial cells affects miRNA expression and trophoblast cell attachment. Reprod Sci. 2020;27:779-86. [DOI:10.1007/s43032-019-00102-8] [PMID] []
14. Ashshi AM, Cooper RJ, Klapper PE, Al-Jiffri O, Moore L. Detection of human herpes virus 6 DNA in fetal hydrops. Lancet. 2000;355(9214):1519-20. [DOI:10.1016/S0140-6736(00)02170-X] [PMID]
15. Aubin JT, Poirel L, Agut H, Huraux JM, Bignozzi C, Brossard Y, et al. Intrauterine transmission of human herpesvirus 6. Lancet. 1992;340(8817):482-3. [DOI:10.1016/0140-6736(92)91801-E] [PMID]
16. Shokoofeh M, Malekshahi SS, Soltanghoraee H. The molecular footprints of BK virus in the product of conception over the second and third gestational trimesters. BMC Res Notes. 2023;16(1):367. [DOI:10.1186/s13104-023-06643-1] [PMID] []
17. Hall CB, Caserta MT, Schnabel KC, Shelley LM, Carnahan JA, Marino AS, et al. Transplacental congenital human herpesvirus 6 infection caused by maternal chromosomally integrated virus. J Infect Dis. 2010;201(4):505-7. [DOI:10.1086/650495] [PMID] []
18. Fjaertoft G, Dahl H, Linde A. Transmission of human herpesvirus 6 (HHV-6) from mother to infant during pregnancy and/or delivery. Reactivation of HHV-6 during pregnancy.• 1310. Pediatr Res. 1997;41(4):221. [DOI:10.1203/00006450-199704001-01329]
19. Dahl H, Fjaertoft G, Norsted T, Wang FZ, Mousavi-Jazi M, Linde A. Reactivation of human herpesvirus 6 during pregnancy. J Infect Dis. 1999;180(6):2035-8. [DOI:10.1086/315115] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |