Volume 3, Issue 3 (2017)
IEM 2017, 3(3): 73-77 |
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RAAF N, Amhis W, Baiod-Chorfi S, Benhassine F, Ouar-Korichi M. Helicobacter pylori in children: Molecular characterization, antibiotic resistance and MLST typing of isolated strains in an Algerian hospital. IEM 2017; 3 (3) :73-77
URL: http://iem.modares.ac.ir/article-4-10426-en.html
URL: http://iem.modares.ac.ir/article-4-10426-en.html
1- 1) Department of Microbiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif, Setif, Algeria
2) Central Laboratory of Clinical Biology, Ibn Ziri Bologhine Hospital, Algiers, Algeria
2- Central Laboratory of Clinical Biology, Ibn Ziri Bologhine Hospital, Algiers, Algeria
3- Pediatrics service, Ibn Ziri Bologhine Hospital, Algiers, Algeria
4- Laboratory of Enterobacteriaceae and Related Bacteria, Institut Pasteur, Algiers Algeria
2- Central Laboratory of Clinical Biology, Ibn Ziri Bologhine Hospital, Algiers, Algeria
3- Pediatrics service, Ibn Ziri Bologhine Hospital, Algiers, Algeria
4- Laboratory of Enterobacteriaceae and Related Bacteria, Institut Pasteur, Algiers Algeria
Abstract: (5400 Views)
Background: Helicobacter pylori infection is generally acquired in childhood. Algeria is a country with a high prevalence of H. pylori infection. The aim of this work was to take stock of H. pylori infection in Algerian children.
Materials and Methods: About 31antral biopsies were cultured, and then antibiotic susceptibility testing was performed. The statuses of cagPAI and vacA s, m, I, and d regions were determined as well as geographical typing was done by MultiLocus Sequence Typing (MLST) method.
Results: Culture was H. pylori positive in 12 children. Only one resistance to clarithromycin and one to metronidazole were detected. Four out of six strains possessed cagPAI, and five out of six strains were identified as vacA s2m2i2d2. The five strains tested by MLST were of the hpEurope type.
Conclusion: This study revealed high prevalence of H. pylori infection and low resistance to antibiotics and reported for the first time in Algeria a genetic typing of H. pylori strains isolated from Pediatrics.
Materials and Methods: About 31antral biopsies were cultured, and then antibiotic susceptibility testing was performed. The statuses of cagPAI and vacA s, m, I, and d regions were determined as well as geographical typing was done by MultiLocus Sequence Typing (MLST) method.
Results: Culture was H. pylori positive in 12 children. Only one resistance to clarithromycin and one to metronidazole were detected. Four out of six strains possessed cagPAI, and five out of six strains were identified as vacA s2m2i2d2. The five strains tested by MLST were of the hpEurope type.
Conclusion: This study revealed high prevalence of H. pylori infection and low resistance to antibiotics and reported for the first time in Algeria a genetic typing of H. pylori strains isolated from Pediatrics.
Received: 2017/06/6 | Accepted: 2017/07/22 | Published: 2017/09/1
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