Volume 4, Issue 1 (2018)                   IEM 2018, 4(1): 27-32 | Back to browse issues page

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Department of Bacteriology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran , mahboob.28@gmail.com
Abstract:   (530 Views)
Introduction: The use of antibiotics in food-producing animals has elevated concerns regarding their potential affect on human health. Resistant Salmonella may be transmitted through the food chain to humans. The aim of this study was to determine the prevalence of tetracycline resistance genes among tetracycline-resistant Salmonella enterica from Iran.
Material and methods: In this experimental study, A total of 4369 stool specimens were collected via rectal swab from hospitalized children under the age of 5 with watery diarrhea, with or without blood, mucus and stomach cramps. Antimicrobial susceptibility profiles of Salmonella isolates were performed and Minimum inhibitory concentration (MIC) of tetracycline was assessed. Bacteria were grown on blood agar at 37°C overnight, and genomic DNA was extracted. For evaluating of PCR products used of 1.5% agarose gel in TBE buffer at for 80min.
Findings: High level of resistance was observed against minocycline (78.5%), tetracycline (76.0%), nalidixic acid (66.6%) and streptomycin (42.0%). The MIC for 46.0% of the isolates was 256µg/ml, while 15.6% showed the MIC of 128µg/ml and the remaining revealed 64µg/ml MIC to tetracycline. Among the 33 tetracycline-resistant isolates the tet(A) or tet(B) genes were detected in 10 (23.8%) and 5 isolates (11.9%), respectively. The tet(A)and tet(B) genes were identified in 2 out of the 42 tetracycline-resistant Salmonella isolates (4.8%). The tet(C) or tet(D)genes were not found among tetracycline-resistant isolates.
Conclusion: Resistance to Salmonella strains is increasing. The predominant tetracycline-resistant gene is tet(A) followed by tet(B).
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Received: 2018/04/10 | Accepted: 2018/05/30 | Published: 2018/05/30