Volume 4, Issue 3 (2018)
IEM 2018, 4(3): 79-85 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Badiri P, Noofeli M, Noormohammadi Z, Nikbin V, Shahbazi T, Shahcheraghi F. Investigation of Genetic Variations in Virulence Factor Genes ptxC, tcfA, and fhaB of Bordetella pertussis Clinical Isolates and Vaccine Strains in Iran. IEM 2018; 4 (3) :79-85
URL: http://iem.modares.ac.ir/article-4-25986-en.html
URL: http://iem.modares.ac.ir/article-4-25986-en.html
1- Microbiology Department, Pasteur Institute of Iran, Tehran, Iran
2- Razi Vaccine & Serum Research Institute, Agricultural Research, Education & Extension Organisation (AREEO), Karaj, Iran
3- Biology Department, Basic Science Faculty, Science & Research Branch, Islamic Azad University, Tehran, Iran
4- Microbiology Department, Medicine Faculty, Shahed University, Tehran, Iran
5- Microbiology Department, Pasteur Institute of Iran, Tehran, Iran , Shahcheraghifereshteh@yahoo.com
2- Razi Vaccine & Serum Research Institute, Agricultural Research, Education & Extension Organisation (AREEO), Karaj, Iran
3- Biology Department, Basic Science Faculty, Science & Research Branch, Islamic Azad University, Tehran, Iran
4- Microbiology Department, Medicine Faculty, Shahed University, Tehran, Iran
5- Microbiology Department, Pasteur Institute of Iran, Tehran, Iran , Shahcheraghifereshteh@yahoo.com
Abstract: (4635 Views)
Aims: Pertussis is an important vaccine preventable disease. It is still a major cause of infant morbidity and mortality in the world. Although the incidence of pertussis was successfully reduced after vaccination, the resurgence of pertussis has been reported in many countries even with high vaccination coverage. Genetic variation in virulence factors is one of the important causes for pertussis reemergence. We investigated genetic characteristics and allele types of 3 important virulence associated genes, including ptxC, tcfA, and fhaB in clinical B. pertussis isolates collected from different provinces of Iran and vaccine strains.
Materials & Methods: Genomic DNA was extracted and ptxC, tcfA, and fhaB gene regions were amplified, using specific PCR primer. DNA sequencing was performed and data were analyzed.
Findings: ptxC2, tcfA2, and fhaB1 were the dominant alleles with 87.5%, 97.5%, and 97.5% frequencies, respectively. Vaccine strains B. pertussis 134 and B. pertussis 509 contain the genotypes ptxC2- tcfA2-fhaB1 and ptxC2- tcfA2-fhaB1.
Conclusion: Results for dominant alleles in ptxC2, tcfA2, and fhaB1 genes in Iran are consistent with dominant alleles of other countries such as Netherland, Finland, and Italy. It seems that ptxC2, tcfA2, and fhaB1 are the dominant circulating alleles in many countries after vaccination period, while vaccine strains have different alleles occasionally. More reported cases in recent years despite high coverage vaccination in Iran and genetic distances between clinical and vaccine strains suggest that antigenic changes in virulence factors possibly have an important role in the survival and evolution of the bacteria.
Materials & Methods: Genomic DNA was extracted and ptxC, tcfA, and fhaB gene regions were amplified, using specific PCR primer. DNA sequencing was performed and data were analyzed.
Findings: ptxC2, tcfA2, and fhaB1 were the dominant alleles with 87.5%, 97.5%, and 97.5% frequencies, respectively. Vaccine strains B. pertussis 134 and B. pertussis 509 contain the genotypes ptxC2- tcfA2-fhaB1 and ptxC2- tcfA2-fhaB1.
Conclusion: Results for dominant alleles in ptxC2, tcfA2, and fhaB1 genes in Iran are consistent with dominant alleles of other countries such as Netherland, Finland, and Italy. It seems that ptxC2, tcfA2, and fhaB1 are the dominant circulating alleles in many countries after vaccination period, while vaccine strains have different alleles occasionally. More reported cases in recent years despite high coverage vaccination in Iran and genetic distances between clinical and vaccine strains suggest that antigenic changes in virulence factors possibly have an important role in the survival and evolution of the bacteria.
Article Type: Original Research |
Subject:
Bacteriology
Received: 2018/04/10 | Accepted: 2019/02/25 | Published: 2018/09/22
Received: 2018/04/10 | Accepted: 2019/02/25 | Published: 2018/09/22
References
1. WHO Publication. Pertussis vaccines: WHO position paper--recommendations. Vaccine. 2011;29(13):2355-6. [Link] [DOI:10.1016/j.vaccine.2010.11.059]
2. Bart MJ, Van Gent M, Van Der Heide HGJ, Boekhorst J, Hermans P, Parkhill J, et al. Comparative genomics of prevaccination and modern Bordetella pertussis strains. BMC Genom. 2010;11:627. [Link] [DOI:10.1186/1471-2164-11-627]
3. Mooi FR. Bordetella pertussis and vaccination: the persistence of a genetically monomorphic pathogen. Infect Genet Evol. 2010;10(1):36-49. [Link] [DOI:10.1016/j.meegid.2009.10.007]
4. Kallonen T, He Q. Bordetella pertussis strain variation and evolution postvaccination. Expert Rev Vaccines. 2009;8(7):863-75. [Link] [DOI:10.1586/erv.09.46]
5. Mooi FR, Van Oirschot H, Heuvelman K, Van Der Heide HG, Gaastra W, Willems RJ. Polymorphism in the Bordetella pertussis virulence factors P.69/pertactin and pertussis toxin in The Netherlands: Temporal trends and evidence for vaccine-driven evolution. Infect Immun. 1998;66(2):670-5. [Link]
6. Parton R. Review of the biology of Bordetella pertussis. Biologicals. 1999;27(2):71-6. [Link] [DOI:10.1006/biol.1999.0182]
7. Guiso N. How to fight pertussis?. Ther Adv Vaccines. 2013;1(2):59-66. [Link] [DOI:10.1177/2051013613481348]
8. Bouchez V, Hegerle N, Strati F, Njamkepo E, Guiso N. New data on vaccine antigen deficient Bordetella pertussis isolates. Vaccines (Basel). 2015;3(3):751-70. [Link] [DOI:10.3390/vaccines3030751]
9. Zarei S, Jeddi Tehrani M, Akhondi MM, Zeraati H, Ferydonfar AA, Nasernia J, et al. Immunogenicity and reactogenicity of two diphtheria-tetanus-whole cell pertussis vaccines in Iranian pre-school children, a randomized controlled trial. Hum Vaccin Immunother. 2013;9(6):1316-22. [Link] [DOI:10.4161/hv.24093]
10. Breu F, Guggenbichler S, Wollmann J. World Health Statistics 2013. Vasa 2013. [Link]
11. Nikbin VS, Jannesar Ahmadi N, Hosseinpour M, Nakhost Lotfi M, Shooraj F, Sadeghpour F, et al. Virulence factors variation among Bordetella pertussis isolates in Iran. Int J Mol Cell Med. 2015;4(2):138-42. [Link]
12. Nikbin VS, Shahcheraghi F, Nakhost Lotfi M, Zahraei SM, Parzadeh M. Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran. Iran J Microbiol. 2013;5(3):209-14. [Link]
13. King AJ, Van Gorkom T, Van Der Heide HG, Advani A, Van Der Lee S. Changes in the genomic content of circulating Bordetella pertussis strains isolated from the Netherlands, Sweden, Japan and Australia: Adaptive evolution or drift?. BMC Genom. 2010;11:64. [Link] [DOI:10.1186/1471-2164-11-64]
14. Mooi FR, He Q, Guiso N. Phylogeny, evolution, and epidemiology of Bordetellae. In: Locht C, editor. Bordetella: Molecular microbiology. Wymondham: Horizon Bioscience; 2007. pp. 17-45. [Link]
15. Sadeghpour Heravi F, Nikbin VS, Shahcheraghi F. Allelic variations between vaccine strains and circulating strains in pxtP of Bordetella pertussis in Iran. Vaccine Res. 2015;2(1-2):19-23. [Link] [DOI:10.18869/acadpub.vacres.2.3.19]
16. Tamura M, Nogimori K, Murai S, Yajima M, Ito K, Katada T, et al. Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model. Biochemistry. 1982;21(22):5516-22. [Link] [DOI:10.1021/bi00265a021]
17. Relman D, Tuomanen E, Falkow S, Golenbock DT, Saukkonen K, Wright SD. Recognition of a bacterial adhesion by an integrin: macrophage CR3 (alpha M beta 2, CD11b/CD18) binds filamentous hemagglutinin of Bordetella pertussis. Cell. 1990;61(7):1375-82. [Link] [DOI:10.1016/0092-8674(90)90701-F]
18. Ishibashi Y, Relman DA, Nishikawa A. Invasion of human respiratory epithelial cells by Bordetella pertussis: Possible role for a filamentous hemagglutinin Arg-Gly-Asp sequence and alpha5beta1 integrin. Microb Pathog. 2001;30(5):279-88. [Link] [DOI:10.1006/mpat.2001.0432]
19. Van Loo IH, Heuvelman KJ, King AJ, Mooi FR. Multilocus sequence typing of Bordetella pertussis based on surface protein genes. J Clin Microbiol. 2002;40(6):1994-2001. [Link] [DOI:10.1128/JCM.40.6.1994-2001.2002]
20. Godfroid F, Denoël P, Poolman J. Are vaccination programs and isolate polymorphism linked to pertussis re-emergence?. Expert Rev Vaccines. 2005;4(5):757-78. [Link] [DOI:10.1586/14760584.4.5.757]
21. Van Amersfoorth SCM, Schouls LM, Van Der Heide HGJ, Advani A, Hallander HO, Bondeson K, et al. Analysis of Bordetella pertussis populations in European countries with different vaccination policies. J Clin Microbiol. 2005;43(6):2837-43. [Link] [DOI:10.1128/JCM.43.6.2837-2843.2005]
22. Van Loo IH, Mooi FR. Changes in the Dutch Bordetella pertussis population in the first 20 years after the introduction of whole-cell vaccines. Microbiology. 2002;148(Pt 7):2011-8. [Link] [DOI:10.1099/00221287-148-7-2011]
23. Mosiej E, Zawadka M, Krysztopa-Grzybowska K, Polak M, Augustynowicz E, Piekarska K, et al. Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959-2013. Eur J Clin Microbiol Infect Dis. 2015;34(1):147-52. [Link] [DOI:10.1007/s10096-014-2216-6]
24. Jung SO, Moon YM, Kim SH, Sung HY, Kwon SJ, Kang YH, et al. Multilocus sequence analysis of housekeeping genes and antigenic determinant genes in Bordetella pertussis strains isolated in Korea. Osong Public Health Res Perspect. 2011;2(2):115-26. [Link] [DOI:10.1016/j.phrp.2011.08.003]
25. Packard ER, Parton R, Coote JG, Fry NK. Sequence variation and conservation in virulence-related genes of Bordetella pertussis isolates from the UK. J Med Microbiol. 2004;53(Pt 5):355-65. [Link] [DOI:10.1099/jmm.0.05515-0]
26. Mooi FR, Van Der Maas NA, De Melker HE. Pertussis resurgence: Waning immunity and pathogen adaptation - two sides of the same coin. Epidemiol Infect. 2014;142(4):685-94. [Link] [DOI:10.1017/S0950268813000071]
27. Simmonds K, Fathima S, Chui L, Lovgren M, Shook P, Shuel M, et al. Dominance of two genotypes of Bordetella pertussis during a period of increased pertussis activity in Alberta, Canada: January to August 2012. Int J Infect Dis. 2014;29:223-5. [Link] [DOI:10.1016/j.ijid.2014.10.028]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |