Volume 4, Issue 4 (2018)
IEM 2018, 4(4): 123-130 |
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1- Department of Biology, Faculty of Basic Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3- Department of Biology, Faculty of Basic Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran , nas.asgharimoghaddam@iauctb.ac.ir
2- Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3- Department of Biology, Faculty of Basic Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran , nas.asgharimoghaddam@iauctb.ac.ir
Abstract: (4844 Views)
Aims: The aim of this study was to identify antibiotic resistant patterns and the prevalence rate of carbapenem resistant genes (imp-1, vim-2, kpc ) in P. aeruginosa strains isolated from burn patients in Shahid Motahari Hospital of Tehran.
Materials & Methods: In this study, 63 P.aeruginosa strains were collected from infected patients. Isolates were identified by biochemical tests and specific 16SrDNA PCR. Antibiotic susceptibility test was performed by standard Kirby-Bauer method according to the CLSI guidelines. The prevalence of imp-1, vim-2, and kpc genes were assessed by PCR.
Findings: All of the isolates were confirmed as P.aeruginosa by phenotypic tests and specific 16SrDNA PCR. Totally, 14 antibiotypes were identified. The highest resistance was observed against to tobramycin, gentamicin, amoxi -clavulanic acid, and cefoxitin (100%) and the most sensitivity was shown against colistin (100%). All of the isolates were multidrug resistant (MDR), 100 and 46% were positive for Extended Spectrum β-Lactamases (ESBL) and Metallo- β-Lactamases (MBLs) respectively. The imp-1 and kpc genes were not detected (0%), while vim-2 gene was present in all of the isolates.
Conclusion: In the current study,the high resistance rate to antibiotics might be due to their overuse for burn patients as a prophylactic or therapeutic agents. Colistin is considered a drug of choice for the treatment of wounds infected by P. aeruginosa in burn patients. In this study, the majority of P. aeruginosa isolates belonged to Antibiotype 1 and possess carbapenemase vim-2. Therefore, to stop this resistance transmission, the prevention and control are apparently essential.
Materials & Methods: In this study, 63 P.
Findings: All of the isolates were confirmed as P.
Conclusion: In the current study,
Article Type: Original Research |
Subject:
Bacteriology
Received: 2018/06/24 | Accepted: 2018/10/5 | Published: 2018/12/20
Received: 2018/06/24 | Accepted: 2018/10/5 | Published: 2018/12/20
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