Volume 2, Issue 2 (2016)                   IEM 2016, 2(2): 8-11 | Back to browse issues page

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1- Department of Microbiology North Tehran Branch, Islamic Azad University, Tehran, IR Iran
2- Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Iran
3- Department of Chemistry, Applied and Scientific University, Pasargad, Iran
Abstract:   (3560 Views)
Background: Pseudomonas aeruginosa is considered an opportunistic pathogen; several reports indicate that the organism can also cause infections in healthy hosts. Four effector proteins have been described in P. aeruginosa: exoU, exoS, exoT, and exoY. These genes that are translated into protein products related to type III secretion systems. Materials and Methods: A total of 134 samples were isolated, and P. aeruginosa was identified using biochemical tests. Bacterial genomic DNA was extracted, and the presence of the exoSand exoUgenes were detected by PCR. Biofilms were formed by culturing P. aeruginosaon glass slides in rich medium. Results: The exoU(73%), exoS (62%) genes were detected from infections caused by P. aeruginosa in urinary tract infection patients. Among the 119 strains isolated from patients with urinary tract infections. Conclusion: An improved understanding of virulence genes and biofilm formation in P.aeruginosa may facilitate the future development of novel vaccines and drug treatments.
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Received: 2016/02/8 | Accepted: 2016/02/1 | Published: 2016/02/8