Volume 3, Issue 1 (2017)
IEM 2017, 3(1): 23-24 |
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Kalantar E, Madani M, Hatami A, Dehghan M H, Ebadi M, Nazari M, et al . Multiplex PCR for Detection of a Successful Pathogen; Acinetobacter baumanni as a Real Threat in Intensive Care Unit of a University Hospital. IEM 2017; 3 (1) :23-24
URL: http://iem.modares.ac.ir/article-4-7963-en.html
URL: http://iem.modares.ac.ir/article-4-7963-en.html
Enayatollah Kalantar1 
, Majid Madani2 , Amir Hatami2 , Mohammad Hossein Dehghan3 , Monireh Ebadi1 , Morteza Nazari2 , Saber Saifei2 , Seyed Mahmoud Amin Marashi * 1
, Majid Madani2 , Amir Hatami2 , Mohammad Hossein Dehghan3 , Monireh Ebadi1 , Morteza Nazari2 , Saber Saifei2 , Seyed Mahmoud Amin Marashi * 1
1- Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
2- Diagnostic Laboratory, Shahid Rajaei Hospital, Alborz University of Medical Sciences, Karaj, Iran.
3- Department of Biochemistry, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
2- Diagnostic Laboratory, Shahid Rajaei Hospital, Alborz University of Medical Sciences, Karaj, Iran.
3- Department of Biochemistry, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Abstract: (3909 Views)
Multidrug resistance in Acinetobacter baumannii is a growing public health concern all over the world. In the current study, the isolation and antimicrobial resistance pattern and detection of blaOXA-51 and lpxC genes by multiplex PCR method was performed.
All the isolates demonstrated high levels of resistance rates to amikacin, ciprofloxacin, meropenem, imipenem, ceftriaxone, gentamicin, and colistin. Screening of two resistance genes by multiplex PCR showed that all the isolates contained blaOXA-51 and lpxC genes. As we previously reported, nosocomial infections caused by A. baumannii isolates are a major cause of morbidity and mortality in our hospital.
All the isolates demonstrated high levels of resistance rates to amikacin, ciprofloxacin, meropenem, imipenem, ceftriaxone, gentamicin, and colistin. Screening of two resistance genes by multiplex PCR showed that all the isolates contained blaOXA-51 and lpxC genes. As we previously reported, nosocomial infections caused by A. baumannii isolates are a major cause of morbidity and mortality in our hospital.
Keywords: Acinetobacter baumannii, Nosocomial infection, multidrug-resistance, blaOXA-51 and lpxC genes
Received: 2016/09/4 | Accepted: 2016/12/5 | Published: 2017/01/1
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