TY - JOUR T1 - Multiplex PCR for Detection of a Successful Pathogen; Acinetobacter baumanni as a Real Threat in Intensive Care Unit of a University Hospital TT - JF - mdrsjrns JO - mdrsjrns VL - 3 IS - 1 UR - http://iem.modares.ac.ir/article-4-7963-en.html Y1 - 2017 SP - 23 EP - 24 KW - Acinetobacter baumannii KW - Nosocomial infection KW - multidrug-resistance KW - blaOXA-51 and lpxC genes N2 - Multidrug resistance in Acinetobacter baumannii is a growing public health concern all over the world. In the current study, the isolation and antimicrobial resistance pattern and detection of blaOXA-51 and lpxC genes by multiplex PCR method was performed. All the isolates demonstrated high levels of resistance rates to amikacin, ciprofloxacin, meropenem, imipenem, ceftriaxone, gentamicin, and colistin. Screening of two resistance genes by multiplex PCR showed that all the isolates contained blaOXA-51 and lpxC genes. As we previously reported, nosocomial infections caused by A. baumannii isolates are a major cause of morbidity and mortality in our hospital. M3 ER -