Infection Epidemiology and Microbiology
Infection Epidemiology and Microbiology
IEM
Medical Sciences
http://iem.modares.ac.ir
1
admin
2588-4107
2588-4115
10.61186/iem
en
jalali
1397
10
1
gregorian
2019
1
1
5
1
online
1
fulltext
en
Isolation and Molecular Characterization of Hydrocarbon Degrading Nocardia Isolated from Hospital Environments in Isfahan Province
<strong>Background</strong>: Biodegradation is the metabolic ability of some microorganisms in degrading or transforming the organic and inorganic contaminants into less harmful and non-hazardous substances, which are then integrated into the natural biogeochemical cycles. Some microorganisms, mainly the members of family <em>Actinomycetes</em>, were found with the capability of transforming and degrading the polluting agents. In this study, three different <em>Nocardia</em> species with the ability to biodegrade organic and inorganic compounds were isolated from soil in Isfahan province.<br>
<strong>Materials & Methods: </strong>The soil samples were collected from the hospital environments. Isolation process was done according to the standard methods. The identification and characterization of the isolates were based on the conventional and molecular methods, including direct sequence analysis of almost full length of 16S rRNA gene.<br>
<strong>Results</strong>: Almost, the complete 16S rRNA gene sequences of the strains under study revealed that the isolates coded as NR6, NR17, NR18, NR25, NR26, and NR28 were the strains of <em>N. cyriacigeorgica</em>; NR7, NR34, and NR50 were the strains of <em>N. coubleae</em>; and NR4 was the strain of <em>N. otitidiscaviarum</em>. The relationship between the isolates under study and standard strain of <em>Nocardia</em> was supported by a phylogenetic tree of 16S rRNA gene.<br>
<strong>Conclusion</strong>: In this study, 10 <em>Nocardia</em> strains with the capability of biotransforming polluting agents were isolated from the hospital environments. It was the first study conducted on the isolation and characterization of <em>Nocardia</em> strains, with the capability of degrading polluting agents, from Iranian hospitals. This study can be considered as a pioneer to develop a new insight about the study of microbial diversity in Iran using an applied approach to deal with environmental challenges.<br>
Nocardia, 16SrRNA, Biodegradation, Hospitals, Environmental samples
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48
http://iem.modares.ac.ir/browse.php?a_code=A-10-46383-1&slc_lang=en&sid=4
ِDavood
Azadi
davood.azadi@khomeinums.ac.ir
1003194753284600125439
1003194753284600125439
Yes
1 Department of Laboratory Sciences, Khomein University of Medical Sciences, Khomein, Iran. 2 Molecular medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
Tahereh
Motalebirad
tmotalebirad@gmail.com
1003194753284600125440
1003194753284600125440
No
Molecular medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
Fatemeh
rezaei
fatemeh.rezaie1997r@gmail.com
1003194753284600125441
1003194753284600125441
No
Department of Laboratory Sciences, Khomein University of Medical Sciences, Khomein, Iran.
Hossein Ali
Rahdar
1003194753284600125442
1003194753284600125442
No
Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.