Infection Epidemiology and Microbiology
Infection Epidemiology and Microbiology
IEM
Medical Sciences
http://iem.modares.ac.ir
1
admin
2588-4107
2588-4115
10.61186/iem
en
jalali
1394
10
1
gregorian
2016
1
1
2
1
online
1
fulltext
en
Study of Mutations in katG and inhA Genes by High Resolution Method in Isoniazid Resistant Mycobacterium tuberculosis Isolates
Background: Tuberculosis is one of the infectious diseases worldwide that has resurgence by the AIDS epidemic and led to the rise of drug-resistant tuberculosis patients. Thus, it seems essential to monitor the drug susceptibility in tuberculosis patients. The new High Resolution Melting (HRM) method is simple, rapid and inexpensive for detection of the mutations responsible for drug resistance in <em>Mycobacterium tuberculosis</em> isolates. In this study, we used HRM method to detect mutations in samples collected from tuberculosis patients. Materials and Methods: Three thousand sputum samples were collected from patients with suspected tuberculosis referred to Iran Remedial Center over a period of 2 years, out of which 2000 samples were found positive for <em>M. Tuberculosis</em> on direct smear. After extraction of genomic DNA from sputums, HRM method was used to detection of mutations in<em> kat</em>G and <em>inh</em>A genes. Results: Our findings showed that 120 out of 2000 positive smear samples were resistant to isoniazid due to mutations in <em>kat</em>G and <em>inh</em>A genes, out of which, 25 mutation was found in <em>inh</em>A gene and 95 mutation in <em>kat</em>G gene. Conclusion: The HRM method is quick, easy and affordable without need of culture and any post PCR process for diagnosing of drug resistance in tuberculosis clinical samples.
Mycobacterium tuberculosis,Isoniazid,High Resolution Melting
4
7
http://iem.modares.ac.ir/browse.php?a_code=A-10-1000-5211&slc_lang=en&sid=4
Zoha
Tavakkoliamol
Zoha
Tavakkoliamol
100319475328460060622
100319475328460060622
Yes
Department of Genetic and Microbiology in UAB University Barcelona Spain investigator in Parc Tauli Hospital, Sabaddel, Spain
Department of Genetic and Microbiology in UAB University Barcelona Spain investigator in Parc Tauli Hospital, Sabaddel, Spain
Ali
Nazemi
Ali
Nazemi
100319475328460060616
100319475328460060616
No
Department of Microbiology, Faculty of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
Department of Microbiology, Faculty of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
Mohammad
Khatamnejad
Mohammad
Khatamnejad
100319475328460060618
100319475328460060618
No
Department of Microbiology, Faculty of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
Department of Microbiology, Faculty of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran