Infection Epidemiology and Microbiology
Infection Epidemiology and Microbiology
IEM
Medical Sciences
http://iem.modares.ac.ir
1
admin
2588-4107
2588-4115
10.61186/iem
en
jalali
1395
7
1
gregorian
2016
10
1
2
4
online
1
fulltext
en
Prevalence of Class 1, 2, and 3 Integrons and Biofilm Formation in Pseudomonas aeruginosa and Acinetobacter baumannii among ICU and non-ICU Patients
<strong>Background: </strong>Infections caused by <em>Pseudomonas aeruginosa</em> or <em>Acinetobacter baumannii</em> are of greatest concern for hospitalized patients, particularly those in intensive care units (ICUs). The aims of this study were to investigate the prevalence of integrons and biofilm formation among <em>P. aeruginosa </em>and <em>A. baumannii</em> isolates collected from ICU and non-ICU inpatients. <br /><strong>Materials and Methods: </strong>A total of 90 <em>P. aeruginosa</em> and 90 <em>A. baumannii </em>isolates were recovered from patients admitted into diverse units of Shahid Mohammadi hospital in Bandar Abbas from January to December 2014. Bacterial identification was carried out by phenotypic methods and PCR. Antibiotic susceptibility was measured by disk diffusion assay. The presence of Class 1, 2, and 3 integrons were evaluated by multiplex-PCR. Biofilm quantification was done by microtiter method. <br /><strong>Results: </strong>The highest number of isolates (48%) were recovered from ICU patients. 81% of <em>P. aeruginosa </em>isolateswere sensitive to piperacillin/tazobactam and ticarcillin, while 60% were resistant to third generation of cephalosporins. In case of <em>A. baumannii, </em>all the isolates were sensitive to colistin, but 98% were resistant to other antibiotics (p≤0.05). Susceptibility to ceftazidime, ticarcillin, imipenem, and piperacillin/tazobactam were higher among isolates obtained from non-ICU patients. Class 1 integron was detected in 13.3% of the <em>P. aeruginosa </em>and 40% of the <em>A. baumannii </em>isolates, while Class 2 integron was harbored by 7 and 6.6% of the isolates, respectively. Furthermore, 23% of the <em>A. baumannii</em> and 12% of the <em>P. aeruginosa </em>isolates showed strong biofilm activity. <br /><strong>Conclusion: </strong>Class 1 integron-positive isolates were resistant to three classes of antibiotics and predominantly observed in specimens collected from ICU patients showing strong biofilm.
ICU,Antibiotic resistance,integrons,Multiplex-PCR
1
7
http://iem.modares.ac.ir/browse.php?a_code=A-4-1000-5273&slc_lang=en&sid=4
Mina
Aryanezhad
Mina
Aryanezhad
100319475328460060449
100319475328460060449
No
Department of Microbiology and Virology, Afzalipuor School of Medicine, Kerman university of Medical Sciences, Kerman, Iran.
Department of Microbiology and Virology, Afzalipuor School of Medicine, Kerman university of Medical Sciences, Kerman, Iran.
Mohammad Reza
Shakibaie
Mohammad Reza
Shakibaie
100319475328460060465
100319475328460060465
Yes
Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran
Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran
Afsaneh
Karmostaji
Afsaneh
Karmostaji
100319475328460060355
100319475328460060355
No
Infectious and Tropical Diseases Research Center, Hormozgan
University of Medical Sciences, Bandar Abbas, Iran
Infectious and Tropical Diseases Research Center, Hormozgan
University of Medical Sciences, Bandar Abbas, Iran
Samane
Shakibaie
Samane
Shakibaie
100319475328460060324
100319475328460060324
No
Students Research Committee, Kerman university of Medical Sciences, Kerman, Iran.
Students Research Committee, Kerman university of Medical Sciences, Kerman, Iran.