Infection Epidemiology and Microbiology
Infection Epidemiology and Microbiology
IEM
Medical Sciences
http://iem.modares.ac.ir
1
admin
2588-4107
2588-4115
10.61186/iem
en
jalali
1396
2
1
gregorian
2017
5
1
3
2
online
1
fulltext
en
Antibiotyping and Genotyping of Pseudomonas aeruginosa Strains Isolated from Mottahari Hospital in Tehran, Iran by ERIC-PCR
<strong>Background: </strong><em>Pseudomonas aeruginosa</em> has become the most common cause of infections in burn patients. The aim of this study was to investigate the antibiotyping and genotyping of <em>P. aeruginosa</em> strains isolated from burn patients in Mottahari hospital during June-October 2016. <br /> <strong>Materials and Methods: </strong>A total of 78 <em>P. aeruginosa</em> strains were collected from wound infected patients. Identification of the isolates was performed by biochemical tests and confirmed by specific 16srDNA PCR. Antimicrobial susceptibility testing was done by disk diffusion method according to the CLSI guidelines. The isolates were then evaluated for genotyping by ERIC-PCR.<br /> <strong>Results:</strong> From a total of 78 collected isolates, 77 isolates (98.7%) were confirmed as <em>P. aeruginosa </em>by specific PCR. We found 4 antibiotypes. The highest resistance was observed to imipenem and gentamicin (~100%), and the most sensitivity was shown to colistin (100%). Overall, MDR phenotype was observed in most of the isolates (98.7%). The PCR of ERIC box produced 52 different patterns and 3 main clusters. Also, 59 (83%), 2 (3%), and 9 (13%) isolates were included in Cluster A, B, and C, respectively, and Cluster A was the predominant ERIC profile.<br /> <strong>Conclusion: </strong>The high resistance to antibiotics in our study may be due to their abundant use as the prophylactic or treatment regimen in wound infections. So appropriate use of antibiotics seems necessary, and colistin is a proper choice for treatment of burn infection. In genotyping, 3 main clusters and 52 different patterns were shown. A majority of the <em>P. aeruginosa</em> strains isolated from burn patients were related and belonged to Cluster A.
P. aeruginosa,burns,Genotyping technique
41
45
http://iem.modares.ac.ir/browse.php?a_code=A-4-18686-1&slc_lang=en&sid=4
Hossein
Fazeli
Hossein
Fazeli
100319475328460048005
100319475328460048005
No
Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
Bahram
Nasr Esfahani
Bahram
Nasr Esfahani
100319475328460048004
100319475328460048004
No
Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
Mahboubeh
Sattarzadeh
Mahboubeh
Sattarzadeh
100319475328460048003
100319475328460048003
No
Motahhari Burn Hospital, Iran University of Medical Sciences, Tehran, IR Iran
Motahhari Burn Hospital, Iran University of Medical Sciences, Tehran, IR Iran
Hajar
Mohammadi Barzelighi
Hajar
Mohammadi Barzelighi
100319475328460048002
100319475328460048002
Yes
Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran