Infection Epidemiology and Microbiology
Infection Epidemiology and Microbiology
IEM
Medical Sciences
http://iem.modares.ac.ir
1
admin
2588-4107
2588-4115
10.61186/iem
en
jalali
1396
6
1
gregorian
2017
9
1
3
3
online
1
fulltext
en
Survival of Dermatophytes in Skin Scales after 10 Years Storage
<strong>Background</strong>:Survival of <em>dermatophytes</em>inskin scales and nail scraps sampled from human lesions could have ecological and epidemiologicalimportance.The aim of this study was to store humanskin scale and nail lesions infected with <em>dermatophyte</em>agents,at -20°C for a long timeand to investigate the re-isolation rate of <em>dermatophytes </em>species from such samples after storage.<br /> <strong>Materials and Methods</strong>:After referral to Medical Mycology Laboratory of Tehran University of Medical Science, the patients were subjected to sampling. Confirmation of <em>dermatophyte</em>positive result in direct smears was done with Potassium hydroxide, then culturing on Sabouraud dextrose agar media was performed, and species were identifiedusing standard procedures, and remaining samples were stored at -20°C. After 10 years, samples were re-examined for the presence and re-isolation of intended fungi according to the standard procedures.<br /> <strong>Results:</strong>From a total of 5 species enrolled in this study, <em>Epidermophyton</em><em> floccosum, <em>Trichophyton mentagrophytes</em></em>, and<em>Tricholosporum</em><em>violaceum</em> survived, and<em>Trichophyton</em><em> rubrum</em> and<em>Trichophyton</em><em>verrucosum</em>lost their viability at -20°C after 10 years. There was a significant difference between the re-isolation rate and the type of <em>dermatophytes</em> (<em>p</em><.05). <em>E. floccosum</em> had the highest re-isolation rate (66.7%), followed by <em>T</em>.<em> mentagrophytes (27%).</em> All of the re-isolated <em>dermatophytes</em> were taken from scales samples, but none from nailscraps samples.<br /> <strong>Conclusion:</strong> Some <em>dermatophytes </em>species including <em>E</em>. <em>floccosum</em> are able to maintain their ability to survive for a long time in human scale samples in certain time and temperature conditions (10 years at -20 °C) and to grow in appropriate conditions. These findings should be considered in ecological and epidemiological studies.
Dermatophyte,Survival,Storage,Scale
96
99
http://iem.modares.ac.ir/browse.php?a_code=A-4-11149-4&slc_lang=en&sid=4
Leila
Hosseinpour
Leila
Hosseinpour
100319475328460062401
100319475328460062401
No
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
Mahdi
Zareei
Mahdi
Zareei
100319475328460062071
100319475328460062071
No
Department of Health, Rescue and Treatment of IR Iran Police Force, Tehran, IR Iran
Department of Health, Rescue and Treatment of IR Iran Police Force, Tehran, IR Iran
Zeinab
Borjian Boroujeni
Zeinab
Borjian Boroujeni
100319475328460062142
100319475328460062142
No
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
Razieh
Yaghoubi
Razieh
Yaghoubi
100319475328460062298
100319475328460062298
No
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
Seyed Jamal
Hashemi
Seyed Jamal
Hashemi
100319475328460062451
100319475328460062451
Yes
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran