Infection Epidemiology and Microbiology
Infection Epidemiology and Microbiology
IEM
Medical Sciences
http://iem.modares.ac.ir
1
admin
2588-4107
2588-4115
10.61186/iem
en
jalali
1395
10
1
gregorian
2017
1
1
3
1
online
1
fulltext
en
Antibiotic Resistance Pattern and Evaluation of blaOXA-10, blaPER-1, blaVEB, blaSHV Genes in Clinical Isolates of Pseudomonas aeruginosa Isolated from Hospital in South of Iran in 2014-2015
<strong>Background:</strong> <em>Pseudomonas aeruginosa</em> is one of the main causes of nosocomial infections with a mortality rate up to 40-50%. Resistance to antibiotics is a global challenge in the treatment of infections caused by this bacterium. The Class A beta-lactamases genes, including <em>bla</em><sub>SHV</sub>, <em>bla</em><sub>PER</sub>, <em>bla</em><sub>VEB,</sub> are the most common causes of resistance in this microorganism. This study was conducted to determine antibiotic resistance pattern and the presence of <em>bla</em><sub>per</sub>, <em>bla</em><sub>veb</sub>, <em>bla</em><sub>shv</sub> and <em>bla</em><sub>oxa-10</sub> genes in clinical isolates of<em> P.</em> <em>aeruginosa</em> isolated from patients in a hospital in Bandar Abbas.<br /> <strong>Materials and Methods:</strong> This cross-sectional study was conducted on 72 <em>P. aeruginosa</em> clinical isolates. Antibiotic susceptibility testing was performed by disk diffusion method according to the clinical Laboratory Standard Institute. MIC (Minimum inhibitory concentration) of ceftazidime was performed by E-Test. Polymerase chain reaction (PCR) was performed to identify <em>bla</em><sub>shv</sub>, <em>bla</em><sub>veb-1</sub>, <em>bla</em><sub>oxa-10,</sub> and <em>bla</em><sub>per-1</sub> genes.<br /> <strong>Results:</strong> Most of the isolates were detected from intensive care unit and urine samples. The highest resistance rate which was observed to sulfamethoxazole and ceftazidime, were 68 (94.44%) and 44 (61.11%), respectively. 27.8% of these isolates were multidrug resistance. Among 44 ceftazidime resistance isolates, 15 isolates (34%) showed MIC ≥32 µg.ml in the E- test. The prevalence rates of genes were 4.16, 12.5, 8.33, and 1.38% for<em> bla</em><sub>Oxa-10</sub>, <em>bla</em><sub>Shv</sub>, <em>bla</em><sub>Veb-1</sub>, and <em>bla</em><sub>Per-1 </sub>genes, respectively.<br /> <strong>Conclusion:</strong> The ceftazidime resistance rate and the prevalence rate of resistance genes in the present study were lower than other Iranian studies. However, isolation of these genes is alarming that excessive use of antibiotics can lead to over expression of resistance genes and bacterial efflux pumps and the emergence of MDR phenotypes.
Pseudomonas aeruginosa,Beta-Lactamase,Genes,Multidrug resistance
1
5
http://iem.modares.ac.ir/browse.php?a_code=A-4-11251-1&slc_lang=en&sid=4
Nahid
Bavasheh
Nahid
Bavasheh
100319475328460065921
100319475328460065921
No
Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
Afsaneh
Karmostaji
Afsaneh
Karmostaji
100319475328460065920
100319475328460065920
Yes
Infectious and Tropical Diseases Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Infectious and Tropical Diseases Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.