Tarbiat Modares University Infection Epidemiology and Microbiology 2588-4107 3 1 2017 01 01 Multiplex PCR for Detection of a Successful Pathogen; Acinetobacter baumanni as a Real Threat in Intensive Care Unit of a University Hospital 23 24 1726 EN Enayatollah Kalantar Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. Majid Madani Diagnostic Laboratory, Shahid Rajaei Hospital, Alborz University of Medical Sciences, Karaj, Iran. Amir Hatami Diagnostic Laboratory, Shahid Rajaei Hospital, Alborz University of Medical Sciences, Karaj, Iran. Mohammad Hossein Dehghan Department of Biochemistry, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. Monireh Ebadi Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. Morteza Nazari Diagnostic Laboratory, Shahid Rajaei Hospital, Alborz University of Medical Sciences, Karaj, Iran. Saber Saifei Diagnostic Laboratory, Shahid Rajaei Hospital, Alborz University of Medical Sciences, Karaj, Iran. Seyed Mahmoud Amin Marashi Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. Journal Article Multidrug resistance in Acinetobacter baumannii is a growing public health concern all over the world. In the current study, the isolation and antimicrobial resistance pattern and detection of blaOXA-51 and lpxC genes by multiplex PCR method was performed. All the isolates demonstrated high levels of resistance rates to amikacin, ciprofloxacin, meropenem, imipenem, ceftriaxone, gentamicin, and colistin. Screening of two resistance genes by multiplex PCR showed that all the isolates contained blaOXA-51 and lpxC genes. As we previously reported, nosocomial infections caused by A. baumannii isolates are a major cause of morbidity and mortality in our hospital.

https://iem.modares.ac.ir/article_1726_74563ba21a90da13dacf2a73e3ddefa7.pdf