Volume 4, Issue 4 (2018)                   IEM 2018, 4(4): 139-145 | Back to browse issues page

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Beheshti N, Ghaffari Far F, Sharifi Z, Eslamirad Z, Farivar Sadr M, Dayer M, et al . Phylogenetic Evaluation and Molecular Variation of Leishmania major and Leishmania tropica Isolated from Different Parts of Iran. IEM. 2018; 4 (4) :139-145
URL: http://iem.modares.ac.ir/article-4-27834-en.html
1- Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran , ghafarif@modares.ac.ir
3- Blood Transfusion Research Center, High Institute for Research & Education in Transfusion Medicine, Tehran, Iran
4- Parasitology Department, Medical Sciences Faculty, Arak University of Medical Sciences, Arak, Iran
5- Parasitology Department, Medical Sciences Faculty, Tehran University of Medical Sciences, Tehran, Iran
6- Parasitology Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran
7- Natural Resources & Environmental Sciences Department, Agriculture & Natural Resources Faculty, Khorasgan (Isfahan) Branch, Islamic Azad University Isfahan, Iran
8- Students Research Committee, Tehran Medical Sciences Branch, Islamic Azad Univesity, Tehran, Iran
9- Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:   (3651 Views)
 ​Aims: The aim of this study was species identification and analysis of species of Leishmania isolated from clinical samples.
Materials & Methods: The samples were collected from patients that were infected from different parts of Iran. After microscopic examination, we used PCR method for the ITS1 (internal transcribed spacer 1) RFLP method (digestion with and for phylogenetic construction, DNA sequencing of
Findings: Two samples from Khorasan province (Mashhad) were Leishmania (L. ), while others were Leishmania major (L. ). L. more variable compared with L. . The molecular sequencing differences between L. to geographical distribution. Based on the results of PCR product in the gel electrophoresis and DNA sequencing for L. L. , the DNA sizes were between 350 and 369bp. The RFLP for L. L. and one respectively. The sequences all samples from central parts are the same, but there is difference with the samples isolated from of Iran.
Conclusion: The sequences of of Leishmania major separated from Damghan and Esfarayen are different from other samples. Similarity of DNA sequences of North-East part of Iran of L. from central parts was 99%. The similarity of two isolates of L. 96%. The most similarity of Leishmania isolated was 95% with Indian isolate and the most similarity for Leishmania major was 99% with Friedlin strain.

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Article Type: Original Research | Subject: Parasitology
Received: 2018/06/4 | Accepted: 2018/10/10 | Published: 2018/12/20

References
1. WHO. The leishmaniases and Leishmania/HIV co-infections [Internet]. Geneva: WHO; 2000 [cited 2004 May 2]. Available from: http://bit.ly/2UE1QYt [Link]
2. Mirzaei A, Rouhani S, Kazerooni P, Farahmand M, Parvizi P. Molecular detection and conventional identification of leishmania species in reservoir hosts of zoonotic cutaneous leishmaniasis in Fars province, South of Iran. Iran J Parasitol. 2013;8(2):280-8. [Link]
3. Erel O, Kocyigit A, Bulut V, Gurel MS. Reactive nitrogen and oxygen intermediates in patients with cutaneous leishmaniasis. Memórias do Instituto Oswaldo Cruz. 1999;94(2):179-83. [Link] [DOI:10.1590/S0074-02761999000200009]
4. Ashford RW, Bates PA. Leishmaniasis in the old world. In Topley and Wilson's Microbiology and Microbial Infections- Ed Collier L, Balows A, M Susman M, editors. Vol. 5 Parasitology – Ed Cox F. E. G. Pkreier J and Wakelin D. Arnold Publishers; 1999. London. [Link]
5. Lainson R, Show JJ. Evolution, classification and geographical distribution. In: Peters W, Killick-Kendrick R, editors. The leishmaniasis in biology and medicine. London: Academic Press; 1987. pp. 1-120. [Link]
6. Al-Jawabreh A, Schoenian G, Hamarsheh O, Presber W. Clinical diagnosis of cutaneous leishmaniasis: A comparison study between standardized graded direct microscopy and ITS1-PCR of Giemsa-stained smears. Acta Tropica. 2006;99(1):55-61. [Link] [DOI:10.1016/j.actatropica.2006.07.001]
7. Fryauff DJ, Hanafi HA, Klena JD, Hoel DF, Appawu M, Rogers W, et al. Short report: ITS-1 DNA sequence confirmation of Leishmania major as a cause of cutaneous leishmaniasis from an outbreak focus in the Ho district, Southeastern Ghana. Am J Trop Med Hyg. 2006;75(3):502-4. [Link] [DOI:10.4269/ajtmh.2006.75.502]
8. Schönian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, et al. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis. 2003;47(1):349-58. [Link] [DOI:10.1016/S0732-8893(03)00093-2]
9. Spanakos G, Piperaki ET, Menounos PG, Tegos N, Flemetakis A, Vakalis NC. Detection and species identification of Old World Leishmania in clinical samples using a PCR-based method. Trans R Soc Trop Med Hyg. 2008;102(1):46-53. [Link] [DOI:10.1016/j.trstmh.2007.05.019]
10. Marfurt J, Niederwieser I, Makia ND, Beck HP, Felger I. Diagnostic genotyping of Old and New World Leishmania species by PCR-RFLP. Diagn Microbiol Infect Dis. 2003;46(2):115-24. [Link] [DOI:10.1016/S0732-8893(03)00040-3]
11. Kazemi Rad E, Mohebali M, Hajjaran H, Rezaei S, Mamishi S. Diagnosis and characterization of Leishmania species in Giemsa-stained slides by PCR-RFLP. Iran J Public Health. 2008;37(1):54-60. [Link]
12. Vaeznia H, Dalimi A, Sadraei J, Pirstani M. Determination of Leishmania species causing cutaneous leishmaniasis in Mashhad by PCR-RFLP method. Arch Razi Inst. 2009;64(1):39-44. [Link]
13. Chaouch M, Fathallah-Mili A, Driss M, Lahmadi R, Ayari C, Guizani I, et al. Identification of Tunisian Leishmania spp. by PCR amplification of cysteine proteinase B (cpb) genes and phylogenetic analysis. Acta Tropica. 2013;125(3):357-65. [Link] [DOI:10.1016/j.actatropica.2012.11.012]
14. Mahdy MA, Al-Mekhlafi HM, Al-Mekhlafi AM, Lim YA, Bin Shuaib NO, Azazy AA, et al. Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen. PLoS One. 2010;5(9). pii:e12879. [Link] [DOI:10.1371/journal.pone.0012879]
15. Hajjaran H, Mohebali M, Teimouri A, Oshaghi MA, Mirjalali H, Kazemi Rad E, et al. Identification and phylogenetic relationship of Iranian strains of various Leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on N-acetylglucosamine-1-phosphate transferase gene. Infect Genet Evol. 2014;26:203-12. [Link] [DOI:10.1016/j.meegid.2014.05.026]

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