Volume 9, Issue 1 (2023)                   IEM 2023, 9(1): 0-0 | Back to browse issues page

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Akinkunmi F, Ajoseh S, Fakorede C, Abegunrin R, Salami W, Lawal A et al . Prevalence of Salmonella blood-stream infection and antimicrobial resistance in Lagos, Nigeria.. IEM 2023; 9 (1)
URL: http://iem.modares.ac.ir/article-4-64069-en.html
1- Department of Microbiology, Faculty of Science, Lagos State University, P.O. Box 0001 Lasu post office, Ojo, Lagos, Nigeria
2- Department of Microbiology, Faculty of Science, Lagos State University, P.O. Box 0001 Lasu post office, Ojo, Lagos, Nigeria , kabiru.akinyemi@lasu.edu.ng
Abstract:   (220 Views)
Aims: The burden of bacteremia in febrile cases is still poorly understood in Nigeria as in many sub-Saharan African countries due o diagnostic limitations. This study aimed at determining the prevalence of Salmonella- bloodstream infections and the antimicrobial resistance patterns of the isolated bacteria from febrile patients in Lagos, Nigeria.
Materials & Methods: Three hundred blood samples were collected from febrile patients attending 4 medical centres between August 2020 and July 2021 in Lagos. Samples were processed the isolates were identified using API 20E kit. From blood positive culture samples, qPCR was used for the detection of Salmonella isolates using a specific primer set. All isolates were subjected to antimicrobial susceptibility tests by standard procedures.  
Findings:  Fifty-five bacterial isolates with six bacterial genera comprising of 4 (4/55) Salmonella isolates, Klebsiella species 23(41.82%), E. coli 6(10.91%), Proteus species 13(23.64%), Serratia species 7(12.73%) and Citrobacter species 2(3.64%) were identified. This study revealed 100% detection of Salmonella isolates with qPCR using invA gene primer set from the blood samples of positive cultures  Salmonella isolates were 100% resistant to Ceftazidime, Cefotaxime, and Doripenem. Multiple drug resistance (MDR) was observed in  Salmonella and other bacterial isolates.
Conclusion:  The study revealed that the method remains the gold standard for diagnosis and qPCR  was found to be highly specific for the detection of Salmonella using the invA primer set. All the bloodstream bacterial pathogens in this study were MDR, thus a need for constant review of antibiotics in medical settings.  Further molecular study on these bacterial isolates is essential.
Article Type: Original Research | Subject: Bacteriology
Received: 2022/09/7 | Accepted: 2023/01/6 | Published: 2023/03/10

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