Prevalence of Campylobacter spp. and their Common Serotypes in 330 Cases of Red-meat, Chicken-meat and Egg-shell in Zanjan City, Iran

Authors
Shiva Modirrousta
Reza Shapouri
Sama Rezasoltani
Hamed Molaabaszadeh
Department of Microbiology, Faculty of Basic and Medical Sciences, Islamic Azad University, Zanjan branch, Zanjan, Iran
Abstract
Background: Campylobacter spp. are the common pathogens that infect human beings via food. These bacteria are vibrio and have been implicated in abortion. Serotyping is the best way for typing with Penner scheme. C. jejuni and C. coli have 65 serotypes. C. coli is common in birds and dogs. Due to high rate of prevalence of Campylobacter in red-meat, chicken-meat and egg-shell, a suitable method to detect their prevalence, the most common species and serotyping group was necessary. This article describes the prevalence of Campylobacter infection, common serotyping group in 330 samples of red- meat, hen-meat and egg-shell. Materials and Methods: With three methods: enrichment, selective Preston and Skirrow and filtration with membrane filters Campylobacter swere incubated. Bacterial species were identified with physiological and biochemical tests. Penner serotyping was defined with reference antiserum Ag-O and direct agglutination. Results: Prevalence of Campylobacter infection was 21(23%) in red meat, 33(27.5%) in hen meat and 38(31.6%) in eggshell. In egg-shell samples: C. jejuni 20, C. coli 14, C. lari 3 and C. concisus 1 case. In meat common Penner serotyping for C. jejuni O2 had the highest rate. In hen, common Penner serotyping: for C. jejuni O3 and in egg-shell for O1, O2 and O3 had the highest rate. Conclusion: Most infection of campylobacter was found in egg-shell; most common species in these three samples were C. jejuni, then C. coli and C. lari. No C. consicus was found in meat but it was found in hen and egg-shells. In common Penner serotyping for C. jejuni O2 and O3 were the most common and for C. coli in meat O49 and in hen and eggshell O5 were the highest.
Keywords
Campylobacter
Serotyping
C. jejuni
C. coli
  1. Lastovica AJ. Emerging Campylobacter spp.: the tip of the Iceberg. ClinMicrobiol News1. 2006; 28(7): 49-56.

  2. Vandamme P. Taxonomy of the family Campylobacteriaceae. ASM Press.Washington, DC, USA. 2000.

  3. Kist M. Impact and management of Campylobacter in human medicine European perspective. Int J Infect Dis. 2000; 6 (Suppl 3): S44-7.

  4. Mattila L, Siitonen A, Kyrönseppä H, Simula I, Oksanen P, Stenvik M, et al. Seasonal variation in etiology of traveler’s diarrhea. J Infect Dis. 1992; 165(2):385-8.

  5. Adekunle OC, Coker AO, Kolawole DO. Incidence, isolation and characterization of Campylobacter species in Osogbo. Biol Med. 2009; 1 (1): 24-7.

  6. Skirrow MB. Diseases due to Campylobacter, Helicobacter and related bacteria. J Comp Patho. 1994; 111(2): 113-49.

  7. Steinhauserova I, Fojtikova K. Serotyping and identification of Campylobacter jejuni and coli strains of human and animal origin using the PCR method. Acta Vet Brno.1999; 68: 149-54.

  8. Rahimifard N, Shoeibi SH, Mirsalehian A, Mehdizadeh M, Saadati SH, Noori Z, et al. Detection of Campylobacter Jejuniin raw meat. Iran J Microbiol. 2009; 1(3): 43-4.

  9. Clark AG, Bueschkens DH. Laboratory infection of chicken eggs with Campylobacter jejuni by using temperature or pressure differentials. Appl Environ Microbiol. 1985; 49(6):1467-71.

  10. Fonseca BB, Soncini RA,Gimarães AR, Rossi DA. Campylobacter spp. in eggs from cloacal swab positive breeder hens. Braz J Microbiol. 2006; 37(4): 573-5.

  11. Gill CO, Harris LM. Contamination of red-meat carcasses by Campylobacter fetus subsp. Jejuni. Appl Environ Microbiol. 1982; 43(5):977-80.

  12. Sjogren E, Lindblom G, Kaijser B. Comparison of different procedures, transport media, and enrichment media for isolation of Campylobacterspecies from healthy laying hens and humans with diarrhea. J ClinMicrobiol. 1987; 25(10):1966-8.

  13. Rahimi E, Tajbakhsh E. Prevalence of Campylobacter spp. in poultry meat in the Esfahan city, Iran. Bulg J Vet Med. 2008; 11(4): 257-62.

  14. Nachamkin I, Barbagallo S. Culture confirmation of Campylobacter spp. by latex agglutination. J ClinMicrobiol. 1990; 28(4):817-8.

  15. Rosef O, Kapperud G, Lauwers S, Gondrosen B. Serotyping of Campylobacter jejuni, Campylobacter coli, and Campylobacter laridis from domestic and wild animals. Appl Environ Microbiol. 1985; 49(6):1507-10.

  16. Petra Luber. Cross-contamination versus undercooking of poultry meat or eggs - which risks need to be managed first? Int J Food Microbiol. 2009; 134(1-2):21-8.

  17. Acuff GR, Vanderzant C, Gardner FA, Golan FA. Examination of turkey eggs, poults and brooder house facilities for Campylobacter jejuni. J Food Prot. 1982; 45(14): 1279-81.