Antibacterial Effect of Thymus kotschyanus Boiss Leaves Extract on Some Gram-Negative Bacteria

Document Type : Original Research

Author
Department of Biology, Faculty of Science, Farhangian University.
Abstract
Aims: The use of medications with plant origin covers a wide variety of maladies and constitutes an alternative way to antibiotic therapy, which seems to be no longer promising due to the widespread antibiotics resistance among the pathogenic microorganisms.

Active principles having antimicrobial activity could be extracted and purified from plants and used in developing new medications. Among several diseases which have historically scourged man, some of the gram-negative bacteria are potentially epidemic and considered as one of the most outstanding causes of diarrhea. Therefore, this study aimed to evaluate the antibacterial activity of Thymus kotschyanus extracts.

Materials & Methods: The antimicrobial effect of T. kotschyanus Boiss leaves extract on some gram-negative bacteria strains was assayed in vitro by the disk diffusion technique. Dried and crushed plant materials were extracted from distilled water by evaporation and distillation. Finally, the antimicrobial assays were carried out for the plant, and the results were compared with an ampicillin disk results.

Findings: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Entrobacter cloacae, Proteus mirabilis, and Shigella dysenteriae were apparently killed by the extract, as judged by the presence of growth inhibition halos in the assays.

The results of minimum inhibitory concentrations (MICs) showed that E. coli and E. cloacae strains were better inhibited by the extract.

Conclusion: The above results were similar to those from ampicillin disk, suggesting that T. kotschyanus Boiss could be used as a source of active principles against some gram-negative bacteria. Therefore, the tested Thymus extract could be considered as a valuable natural antibacterial source, which seems to be applicable in both medicine and food industry.

Keywords

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1. Fazly-Bazzaz BS, Khajehkaramadin M and Shokooheizadeh HR. In vitro antibacterial activity of Rheum ribes extract obtained from various plant parts against clinical isolates of Gram-negative pathogens. Iranian J. Pharm. Res. 2005; 2: 87-91.
2. Alizadeh A, Alizadeh O, Sharafzadeh SH, Mansoori S. Effect of different ecological environments on growth and active substances of garden Thyme. Advances in Environmental Biology. 2011; 5(4): 780-783.
3. Naghdibadi H, Malekzadeh TM. Areview on oregano plant (Thymus vulgaris L). Herb Q. 2003; 8: 1-12.
4. Akhoondzadeh BA, Razavilar V, Misaghi A, Radmehr B, Abbasifar R, Yazdani D, Akhoondzadeh BSH. Effect of shiraz oregano(Zataria Multiflora Boiss) on growth probability of Staphylococcus aerus in heart and brain broth. Herbs Q. 2004; 10:53-60.
5. Karman S, Digark M, Ravid V, Ilcim A. antibacterial and antifungal activity of essential oils of Thymus revolatus Celak from Turkey. Ethno.pharmacol.J. 2001; 76: 183- 186.
6. Bagamboula CF, Uyttendaele M, Deberere J. Inhibitory effect of thyme and Basil essential oils, Carvacrol, thymol, estragol, linalool and p-cymene towards Shigella Sonnei and S.flexneri. Food Microbiol. 2004; 21: 33-42.
7. Dakhili M, Zahraee ST, Torabi GM, khavari A. Evaluation of antimicrobial effects of four herbal essences on Salmonella typhi Murium and comparison with current antibiotics in veterinary. Herbs Q. 2006; 20:21-26.
8. Nostro A, Germanò MP, D’Angelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Appl.Microbiol. 2000; 30:379-384.
9. Rassoli I, Mirmostafa SA. Antibacterial properties of thymus pubescens. Herbs Q. 2004; 9: 44-48.
10. Zaidi MA and Crow SA. Biologically active traditional medicinal herbs from Balochistan, Pakistan. J. Ethnopharmacol.2005; 96: 331-334.
11. Vila R. Flavonoids and further polyphenols in the genus Thymus. In: Stahl- Biskup E and Saez F (eds.) Thyme, Taylor and Francis, London. 2002; 144-75.
12. Proestos C, Chorianopoulos N, Nychas GJ and Komaitis M. RP-HPLC analysis of the phenolic compounds of plant extracts: Investigation of their antioxidant capacity and antimicrobial activity. J. Agric. Food Chem. 2005; 53: 1190-1195.
13. Marin PD, Grayer RJ, Kite GC, Matevski V. External leaf flavonoids of Thymus species from Macedonia. Biochem Syst Ecol. 2003; 31:1291–1307.
14. Özç elik B, Orhan I and Toker G. Antiviral and antimicrobial assessment of some selected flavonoids. Z. Naturforsch. 2006; 61: 632-638.
15. Maheswara M, Rao YK, Rao VM and Rao CV. Antibacterial activity of acylated flavonol glycoside from Waltheria indica. Asian J. Chem. 2006; 18: 2761-2765.
16. Friedman M.Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas. Mol. Nutr. Food Res. 2007; 51: 116-34.
17. Palombo EA. Traditional medicinal plant extracts and natural products with activity against oral bacteria: Potential application in the prevention and treatment of oral diseases. Evid Based Complement Altern Med. 2011; 2011:680354.
18. Suurbaar J, Mosobil R, Donkor AM. Antibacterial and antifungal activities and phytochemical profile of leaf extract from different extractants of Ricinus communis against selected pathogens. BMC Res Notes. 2017; 10(1):660-666.
19. Morteza-Semnani K, Rostami B and Akbarzadeh M. Essential oil composition of Thymus kotschyanus and T. pubescens from Iran. J. Essen. Oil Res. 2006; 18: 272-274.
20. Nabavi SM, Nabavi SF, Alinezhad H, Zare M, Azimi R. Biological activities of flavonoid-rich fraction of Eryngium caucasicum Trautv. Eu Rev Med Pharmacol Sci. 2012; 16:81–87.
21. Nickavar B, Esbati N. Evaluation of the antioxidant and antibacterial capacity and phenolic content of three Thymus Species. J Acupunct Meridian Stud. 2012; 5:119–125.
22. Mohammed MJ, Al-Bayati FA. Isolation and identification of antibacterial compounds from Thymus kotschyanus aerial parts and Dianthus caryophyllus flower buds. Phytomedicine. 2009; 16:632–637.
23. Saint-Ruf C, Crussard S, Franceschi C, Orenga S, Ouattara J, Ramjeet M, Surre J and Matic I. Antibiotic Susceptibility Testing of the Gram-Negative Bacteria Based on Flow Cytometry. Front Microbiol. 2016; 7: 1121.
24. Bhatt P, Negi PS. Antioxidant and antibacterial activities in the leaf extracts of Indian Borage (Plectranthus amboinicus) Food Nutr Sci. 2012; 3:146–152.
25. Bagci E, Baser KHC. Study of the essential oils of Thymus haussknechtii Velen and Thymus kotschyanus Boiss. et Hohen var. kotschyanus (Lamiaceae) taxa from the eastern Anatolian region in Turkey. Flavour Fragr J. 2005; 20:199–202.
26. Turumtay EA, Islamoglu F, Cavus D, Sahin H, Turumtay H, Vanholme B. Correlation between phenolic compounds and antioxidant activity of Anzer tea (Thymus praecox Opiz subsp. caucasicus var. caucasicus) Ind Crop Prod. 2014; 52:687–694.
27. Thoppil JE, Minija J, Tajo A, Deena MJ. Antimicrobial activities of Eusteralis
deccanensis and E. quadrifolia essential oils. Environ.Biol.J. 2003; 24(2): 211-220.
28. Shams S, Mehdipour A, Kermani S, Ghorbani H, Ragolia S. Anti-Biofilm Activity of Punica granatum, Ricinus communis, and Allium sativum Plant Extracts on Streptococcus mutans. Infection Epidemiology and Microbiology. 2018; 4(2):67-72.
29. Manandhar S, Luitel S, and Dahal RK. In Vitro Antimicrobial Activity of Some Medicinal Plants against Human Pathogenic Bacteria. J Trop Med. 2019; 2019: 1895340.