Simultaneous Comparison of Serological and Molecular Results of HBV Test in Patients Referring to the Pathobiology Laboratory

Document Type : Original Research

Authors
R. Sayadi Boroujeni 1
M. Papizadeh 2
M. Talebi 3
M. R. Pourshafie 2
1 Department of Genetics, Faculty of Basic Science, Shahrekord University, Shahrekord, Iran
2 Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
3 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Iran
Abstract
Aims: Hepatitis B Virus (HBV) has infected more than million hundreds of people worldwide. Hence, a high rate of morbidity and mortality caused by liver-related diseases is due to HBV infection. However, a strong and effective treatment should be based on an accurate and correct diagnostic method. Hence, the present research provided a multidimensional study comparing and analyzing patients’ molecular and serological tests results.

Materials & Methods: In this research, the HBV DNA molecular tests results were studied by examining patients’ gender, age, and HBsAg strip results.

Findings: Among the female patients (29 persons) studied in this research, 55.1% were positive for HBV DNA and HBsAg strip tests, and 17.3% were negative for both tests. Also, among the male patients (44 persons), 65.9% were positive, and 6.8% were negative for both tests.

Conclusion: The present study results shed light on the correlation between the HBV DNA and HBsAg tests. Also, the significance of HBV DNA tests was highlighted for particular diagnostic purposes and for the differentiation and interpretation of the pathophysiological conditions of patients with hepatitis B.

Keywords

Subjects

1. Schaefer S: Hepatitis B virus taxonomy and hepatitis B virus genotypes. World J Gastroenterol 2007, 13:14-21.
2. Seeger C, Mason WS: Hepatitis B Virus Biology. Microbiology and Molecular Biology Reviews 2000, 64:51.
3. Nassal M, Schaller H: Hepatitis B virus replication. Trends in Microbiology 1993, 1:221-228.
4. Schweitzer A, Horn J, Mikolajczyk RT, Krause G, Ott JJ: Estimations of worldwide prevalence of chronic hepatitis B virus infection: a systematic review of data published between 1965 and 2013. Lancet 2015, 386:1546-1555.
5. Fourati S, Pawlotsky J-M: Recent advances in understanding and diagnosing hepatitis B virus infection. F1000Research 2016, 5:F1000 Faculty Rev-2243.
6. Song JE, Kim DY: Diagnosis of hepatitis B. Ann Transl Med 2016, 4:338.
7. Hepatitis B Virus [http://www.who.int/news-room/fact-sheets/detail/hepatitis-b]
8. Trépo C, Chan HLY, Lok A: Hepatitis B virus infection. The Lancet 2014, 384:2053-2063.
9. Candotti D, Laperche S: Hepatitis B Virus Blood Screening: Need for Reappraisal of Blood Safety Measures? Frontiers in medicine 2018, 5:29-29.
10. Cui Y, Zhang T, Yan Y, Liu K: Identification of a mutation in Hepatitis B virus surface antigen capable of evading ELISA screening. Genet Mol Res 2016, 15.
11. Caligiuri P, Cerruti R, Icardi G, Bruzzone B: Overview of hepatitis B virus mutations and their implications in the management of infection. World journal of gastroenterology 2016, 22:145-154.
12. Hadziyannis E, Laras A: Viral Biomarkers in Chronic HBeAg Negative HBV Infection. Genes 2018, 9:469.
13. Hatzakis A, Magiorkinis E, Haida C: HBV virological assessment. J Hepatol 2006, 44:S71-76.
14. Burns GS, Thompson AJ: Viral hepatitis B: clinical and epidemiological characteristics. Cold Spring Harb Perspect Med 2014, 4:a024935.
15. de Franchis R, Hadengue A, Lau G, Lavanchy D, Lok A, McIntyre N, Mele A, Paumgartner G, Pietrangelo A, Rodes J, et al: EASL International Consensus Conference on Hepatitis B. 13-14 September, 2002 Geneva, Switzerland. Consensus statement (long version). J Hepatol 2003, 39 Suppl 1:S3-25.
16. Lai CL, Ratziu V, Yuen MF, Poynard T: Viral hepatitis B. Lancet 2003, 362:2089-2094.
17. Galli C, Orlandini E, Penzo L, Badiale R, Caltran G, Valverde S, Gessoni G: What is the role of serology for the study of chronic hepatitis B virus infection in the age of molecular biology? J Med Virol 2008, 80:974-979.
18. Gholami Parizad E, Gholami Parizad E, Khosravi A, Amraei M, Valizadeh A, Davoudian A: Comparing HBV Viral Load in Serum, Cerumen, and Saliva and Correlation With HBeAg Serum Status in Patients With Chronic Hepatitis B Infection. Hepat Mon 2016, 16:e30385.
19. Esmaeelzadeh A, Saadatnia H, Memar B, Mokhtari Amirmajdi E, Ganji A, Goshayeshi L, Meshkat Z, Pasdar A, Vosoughinia H, Farzanehfar M, et al: Evaluation of serum HBV viral load, transaminases and histological features in chronic HBeAg-negative hepatitis B patients. Gastroenterol Hepatol Bed Bench 2017, 10:39-43.
20. Khamesipour A, Amiri ZM, Kafiabad SA, Saadat F, Mansour-ghanaei F, Esteghamati AR, Shakib RJ: Frequency of hepatitis B virus DNA in anti-HBc positive, HBsAg negative blood donors in Rasht, northern Iran. Transfus Apher Sci 2011, 45:195-197.
21. Samiee S, Kanavi MR, Javadi MA, Bagheri A, Balagholi S, Hashemi MS: Real Time Polymerase Chain Reaction for Hepatitis B Screening in Donor Corneas in the Central Eye Bank of Iran. J Ophthalmic Vis Res 2018, 13:392-396.
22. Pourazar A, Salehi M, Jafarzadeh A, Arababadi MK, Oreizi F, Shariatinezhad K. Detection of HBV DNA in HBsAg Negative Normal Blood Donors. Iran J Immunol 2005, 2(3):172-6.
Infection Epidemiology and Microbiology
Volume 5, Issue 2
February 2019
Pages 53-58