Identification of Mycoplasma synoviae by PCR and Culture Methods and Determination of the Prevalence of P1, P30, and P116 Virulence Genes in the Isolates

Document Type : Original Research

Authors
N. salmani zadeh korani
B. kheirkhah
Department of Microbiology, Shiraz branch, Islamic Azad University, Shiraz, Iran
Abstract
Aims: Mycoplasma synoviae, as one of the main pathogens of birds, causes a lot of economic losses to the poultry industry. This study aimed to identify M. synoviae strains in clinical samples by PCR and culture methods.

Materials & Methods: A total of 135 samples were randomly collected from the respiratory tracts of female broilers in industrial poultry farms in Kerman, Iran during the first six months of 2016. Samples were cultured on Frey and PPLO broth media. Then PCR method was performed to identify Mycoplasma genus and synoviae species. Finally, multiplex PCR was performed to determine the prevalence of P1, P30, and P116 virulence genes.

Findings: In this study, 17 (32%) out of 53 poultry samples were positive for the presence of Mycoplasma genus by culture method, whereas according to the PCR results, 25 (47%) out of 53 samples were confirmed as Mycoplasma genus, among which 13 samples (25%) were identified as M. synoviae species. Among the strains confirmed as M. synoviae, the prevalence rate of P1, P30, and P116 genes was 7 (53.8%), 6 46.1%), and 5 (38.46%), respectively.

Conclusion: According to the PCR and culture methods results, the prevalence of M. synoviae strains was high in industrial poultry farms, Kerman, Iran. The PCR results revealed a higher prevalence rate for this bacterium, suggesting that this method may be more reliable than culture method.

Keywords

Subjects

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Infection Epidemiology and Microbiology
Volume 5, Issue 3
September 2019
Pages 19-26