Determination of Antibiotic Resistance Patterns of Salmonella Serotypes Isolated from Broilers in Kermanshah Province

Document Type : Original Research

Authors
F. Mohammadi 1
M. Karimi Dehkordi 2
1 Department of veterinary, Agriculture Faculty, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Abstract
Objectives: The present study aimed to investigate the prevalence of Salmonella serotypes in slaughtered broilers in Kermanshah province and investigate the susceptibility of isolated Salmonella to antibiotics.

Methods: In this cross-sectional, 10 carcasses were randomly selected from each of 25 slaughtered broiler flocks that were obtained from their cloacae. This study was conducted in 2014 in Kermanshah province. Samples were transferred into the Selenite F enrichment broth and incubated at 43 °C for 12-18 h. Antibiotic susceptibility pattern of all isolates to be performed using the disk diffusion method. Also, the agglutination test on slide was performed on Salmonella isolates using the specific antisera to detect Salmonella serotypes.

Findings: In the present study, 250 (33%) samples of Salmonella were isolated from 750 cloacal samples of broilers. After serotyping, the most frequent serotype was as follow: Enteritidis serotype (55.2%), Infantis serotype (38%) and Typhimurium serotype (25.6%). The results of antibiotic susceptibility pattern indicated that 100% of strains were susceptible to gentamicin, enrofloxacin, imipenem and ceftriaxone; and the highest resistance was observed to nalidixic acid and nitrofurantoin. Furthermore, 202 (80%) out of 250 Salmonella isolates had multiple resistance to three or more antibiotics and accounted as MDR isolates.

Conclusion: According to the results of the present study, it is essential to detect pollution sources and pathogenic serotypes, the possibility of transferring Salmonella through poultry to humans and increasing the frequency of resistant isolates to antibiotics. It is strongly recommended to avoid the overuse of antibiotics without performing antibiotic susceptibility patterns.

Keywords

Subjects

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Infection Epidemiology and Microbiology
Volume 5, Issue 4
December 2019
Pages 25-32