ELISA and Copan Based Evaluation and Analysis of Antibiotic Residues in Cattle Milk in Qazvin, Iran

Document Type : Original Research

Authors
Z. Nemati Niko 1
G. Jahed Khaniki 2
M. Alikord 2
E. MolaeeAghaee 2
1 Department of Food Hygiene and Safety, School of Health, Qazvin University of Medical sciences, Qazvin, Iran
2 Food safety & Hygiene Division, Environmental Health Engineering Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
10.29252/iem.6.3.219
Abstract
Aims: Daily consumption of milk containing antibiotic residues has become a great public health concern. This study aimed to evaluate the antibiotic residues in cattle raw milk using Copan milk test and specific enzyme-linked immunosorbent (ELISA) kits.

Materials & Methods: In this study, 92 milk samples were collected from Qazvin province, Iran in two seasons during 2019. All the samples were analyzed by the Copan milk test and competitive ELISA kits to evaluate gentamicin, tetracycline, tylosin, chloramphenicol, penicillin, and sulfonamide residues in milk.

Findings: The results showed that 45 milk samples (48.91%) were positive for the presence of antibiotic residues. Samples collected in summer were significantly (p<.05) more contaminated with antibiotic residues (30.43%) than those collected in winter (18.47%). The highest mean contamination was related to sulfonamide (13.72±1.21 ng/mL), followed by gentamicin (13.24±2.81 ng/mL) and tylosin (13.15±1.37 ng/mL) residues, and the lowest mean contamination was related to penicillin residues (0.007±0.002 ng/mL). The limit of detection (LOD) was 2, 2.5, 0.02, 8, 0.4, 0.08, and 13 ng/mL for gentamicin, tylosin, chloramphenicol, sulfamethazine, tetracycline, penicillin, and flumequine, respectively. Chloramphenicol residues were above the maximum residue limit (MRL) according to the Codex and European Commission (EC) (0 ng/mL) in all the samples. Sulfonamides residues were above the Codex MRL (25 ng/mL) in 33.33% of the samples.

Conclusion: The results shows that monitoring of antibiotic residues in milk and dairy products is necessary. Indeed, examining the amount of antibiotic residues in dairy products could be an important aspect of their monitoring.

Keywords

Subjects

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Infection Epidemiology and Microbiology
Volume 6, Issue 3
August 2020
Pages 219-227