The Frequency and Tissue Distribution of Ornithobac-terium rhinotracheale in different Organs of Turkeys

Document Type : Original Research

Authors
M. Karimi-Dehkordi 1
H. Hashemi 2
M. Haj Salehi 2
M. Gholami-Ahangaran 1
A. Ahmadi-Dastgerdi 3
1 Department of clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 Department of Food Science and Technology, Ardestan Branch, Islamic Azad University, Ardestan, Iran
10.52547/iem.7.1.45
Abstract
Background: This study was designed to investigate the frequency and tissue distribution of Ornithobacterium rhinotracheale (ORT) in turkeys with respiratory syndrome in Isfahan province.

Materials & Methods: Totally, samples were taken from the trachea, lung, air sac, infraorbital sinus, hock joint, blood of heart, brain, liver, spleen, intestine, and kidney of 30 turkey flocks. After DNA extraction, a 787 bp fragment of 16S rRNA gene of ORT was amplified.

Findings: The PCR results revealed that 53% of turkeys were infected by ORT. The results showed that although ORT was mainly found in the respiratory tract, it could be systemic and infect some other organs, including the joints, brain, liver, spleen, and blood of heart, but could not infect the intestines and kidneys.

Conclusion: Due to the lack of a clear pattern in tissue distribution of ORT among clinical samples, it seems that other factors play a role in ORT tissue distribution, such as dose, route, type of infection, and probably prevalent serotype.

Keywords

Subjects

1. Vandamme P, Segers P, Vancanneyt M, van Hove K, Mutters R, Hommez J, et al. Ornithobacterium rhinotracheale gen. nov., sp. nov., isolated from the avian respiratory tract. Int J Syst Bacteriol. 1994;44(1):24-37.
2. Tahseen A. Ornithobacterium rhinotracheale developing into a serious infection. World Poult Misset. 1997;13:47-8.
3. Kursa, O., Tomczyk, G., Sawicka-Durkalec, A., Giza, A., & Słomiany-Szwarc, M. (). Bacterial communities of the upper respiratory tract of turkeys. Sci Rep. 2021; 11(1): 1-11.
4. Van Empel P, Hafez H. Ornithobacterium rhinotracheale: a review. Avian Pathol. 1999;28(3):217-27.
5. van Empel P, Vrijenhoek M, Goovaerts D, van den Bosch H. Immunohistochemical and serological investigation of experimental Ornithobacterium rhinotracheale infection in chickens. Avian Pathol. 1999;28(2):187-93.
6. Chin RP, and R. Droual. . Ornithobacterium rhinotracheale infection. In: Diseases of poultry, 10th
ed. B. W. Calnek, ed. Iowa State University Press, Ames, IA. pp. 1012-1015. 1997.
7. van Veen L, van Empel CP, Fabria T. Ornithobacterium rhinotracheale, a primary pathogen in broilers. Avian Dis. 2000;44(4):896-900.
8. Suzuki K, Petruccelli MÁ, Trenchi G, Giossa G, Rodríguez G, Trenchi H. Flock-level seroprevalence against Ornithobacterium rhinotracheale among broilers in Uruguay. Int J Poult Sci. 2010; 9:100-110.
9. Allymehr M. Seroprevalence of Ornithobacterium rhinotracheale infection in broiler and broiler breeder chickens in West Azerbaijan Province, Iran. J Vet Med A Physiol Pathol Clin Med. 2006;53(1):40-2.
10. Asadpour Y, Bozorgmehrifard MH, Pourbakhsh SA, Banani M, Charkhkar S. Isolation and identification of Ornithobacterium rhinotracheale in broiler breeder flocks of Guilan province, north of Iran. Pak J Biol Sci. 2008;11(11):1487-91.
11. Ghanbarpour R, Salehi M. Sero-prevalence and identification of Ornithobacterium rhinotracheale in broiler flocks in south-eastern Iran. Trop Anim Health Prod. 2009;41(8):1679-83.
12. Doosti A, Sharifzadeh A, Ghasemi H, Vaez J. Molecular identification of Ornithobacterium rhinotracheale in turkeys in Isfahan province of Iran. Afr J Biotechnol. 2011;10(40):7911-4.
13. Ozbey G, Ongor H, Turgut-Balik D, Celik V, Kilic A, Muz A. Investigations on Ornithobacterium rhinotracheale in broiler flocks in Elazig province located in the East of Turkey. Vet Med. 2004;49:305-11.
14. Roepke DC, Back A, Shaw DP, Nagaraja KV, Sprenger SJ, Halvorson DA. Isolation and identification of Ornithobacterium rhinotracheale from commercial turkey flocks in the upper Midwest. Avian Dis. 1998;42:219-21.
15. Asadi J, Gholami-Ahangaran, M., & Momtaz, H. Molecular detection of Ornithobacterium rhinotracheale in quails of Isfahan province. Iran Clinical Pathol. 2018;12(1):1-7.
16. Shabani A, Gholami-Ahangran M, Momtaz H. Molecular detection Of Ornithbacterium rhinotracheale and Newcastle disease virus In ostriches of Isfahan province. 2017; 11(2): 97-106.
17. Kilic A, Timurkaan N, Ertas H, Yilmaz F. Pathological examination and bacterial reisolation by culture and PCR of experimental Ornithobacterium rhinotracheale infection in broiler chickens. Revue Méd Vét. 2009;160(3):140-4.
18. Thachil A, Velayudhan B, Shaw D, Halvorson D, Nagaraja K. Pathogenesis of Ornithobacterium rhinotracheale in egg-laying hens with coexisting infectious bronchitis virus and Escherichia coli infections. J Appl Poult Res. 2009;18(4):780-8.
19. Zbikowski A, Szeleszczuk P, Binek M. Tissue tropism of Ornithobacterium rhinotracheale in chickens determined by culture and nucleic acid detection. Pol J Microbiol. 2013;62(2):205-9.
Infection Epidemiology and Microbiology
Volume 7, Issue 1
January 2021
Pages 45-51