Evaluation of the Presence of fimH and bfpA Genes in Escherichia coli Isolated from Pregnant Women

Document Type : Original Research

Authors
F. Keshavarzi 1
R. Rahmani 2
F. Karimpour 3
1 1. Department of biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2 Department of biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
3 Central office of Legal Medicine, Sanandaj, Iran
10.52547/iem.7.4.297
Abstract
Backgrounds: Escherichia coli (E. coli) is one of the most abundant bacteria in human and animal infections. Many virulence genes in E. coli intensify its infectivity. This study explored the presence of two pathogenic genes, including fimH and bfpA, in E. coli strains isolated from pregnant women.

Materials & Methods: From autumn 2016 to spring 2017, a total of 100 E. coli isolates were collected from clinical samples (116) of pregnant women. The strains were identified using biochemical tests (catalase, Simmons citrate, indole, mobility, H2S, MR, VP, TSI, and urease). The presence of pathogenic genes in these isolates was examined using colony PCR method. Finally, the relationship between the gene and the site of infection was analyzed in SPSS-23 software.

Findings: PCR results indicated that out of 100 E. coli samples, 15 were bfpA positive (15%), and 64 were fimH positive (64%). A significant relationship was found between the presence of bfpA gene and samples taken from urine (p<.001), blood (p=.049), and stool (p<.001).

Conclusion: None of the urinary strains harbored the bfpA gene, while the strains isolated from stool had a significant relationship with the presence of bfpA gene (OR = 18.667), which confirms that this gene is of great importance for EPEC (enteropathogenic E. coli). There was also a significant relationship between blood-isolated strains and the presence of bfpA gene. A significant relationship was also found between the fimH gene and strains isolated from urine samples (OR=36.733), while no relationship was observed between the presence of fimH gene and blood-isolated strains.

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1.Abbasi, M., Aslani, M. M., Mostafavi, E., Alikhani, M. Y., & Nikbin, V. S. (2013). Determination of Adhesion Virulence Factors of Enteropathogenic Escherichia coli (eaeA-, bfpAA-) Isolates from Asymptomatic Individuals Compared to those with Diarrhea. Modares Journal of Medical Sciences: Pathobiology, 15(4), 99-108.
2.Abdi, A., & Rashki, A. (2014). Comparison of Virulence Factors Distribution in Uropathogenic E. coli Isolates From Phylogenetic Groups B2 and D. Int J Enteric Pathog, 2(4), e21725.
3.Abed, Z. A., Habib, K. A., & Abass, Z. N. (2014). Genotypic Study of Two Virulence Factors fimH and kpsMTII in Uropathogenic Escherichia coli Isolates from Children Patients with Urinary Tract Infections. Baghdad Science Journal, 11(4), 1475-1480.
4.Bieber, D., Ramer, S., Wu, C., Murray, W., Tobe, T., Fernandez, R., & Schoolnik, G. K. (1998). Type IV pili, transient bacterial aggregates, and virulence of 6 enteropathogenic Escherichia coli. Science, 280, 2114-2118.
5.Choudhury, D., Thompson, A., Stojanoff, V., Langermann, S., Pinkner, J., Hultgren, S. J., & Knight, S. D. (1999). X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli. Science, 285(5430), 1061-1066.
6.Cleary, J., Lai, L.-C., Shaw, R. K., Straatman-Iwanowska, A., Donnenberg, M. S., Frankel, G., & Knutton, S. (2004). Enteropathogenic Escherichia coli (EPEC) adhesion to intestinal epithelial cells: role of bundle-forming pili (BFPA), EspA filaments and intimin. Microbiology, 150(3), 527-538.
7.Hyland, R. M., Sun, J., Griener, T. P., Mulvey, G. L., Klassen, J. S., Donnenberg, M. S., & Armstrong, G. D. (2008). The bundlin pilin protein of enteropathogenic Escherichia coli is an N‐acetyllactosamine‐specific lectin. Cellular microbiology, 10(1), 177-187.
8.Khursigara, C., Abul-Milh, M., Lau, B., Giron, J., Lingwood, C., & Foster, D. B. (2001). Enteropathogenic Escherichia coliVirulence Factor Bundle-Forming Pilus Has a Binding Specificity for Phosphatidylethanolamine. Infect Immun, 69(11), 6573-6579.
9.Klemm, P. (1994). Fimbriae Adhesion, Genetics, Biogenesis, and Vaccines: CRC Press.
10.Klemm, P., & Schembri, M. A. (2000). Bacterial adhesins: function and structure. Int J Med Microbiol, 290(1), 27-35. doi: 10.1016/s1438-4221(00)80102-2
11.Knutton, S., Rosenshine, I., Pallen, M. J., Nisan, I., Neves, B. C., Bain, C., . . . Frankel, G. (1998). A novel EspA‐associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells. T
12.Giron, J. A., Ho, A., & Schoolnik, G. K. (1991). An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science, 254(5032), 710-713.
13.Rono, S. J., Kakai, R., Esamai, F., Mibei, E., & Odundo, E. (2014). PATHOTYPES AND VIRULENCE MARKERS IN ESCHERICHIA COLI ASSOCIATED WITH DIARRHOEA AMONG HIV SEROPOSITIVE AND SERONEGATIVE CHILDREN BELOW FIVE YEARS IN WESTERN KENYA. European Scientific Journal, 10(27).
14.Saldana, Z., Erdem, A. L., Schüller, S., Okeke, I. N., Lucas, M., Sivananthan, A., . . . Girón, J. A. (2009). The Escherichia coli common pilus and the bundle-forming pilus act in concert during the formation of localized adherence by enteropathogenic E. coli. J Bacteriol, 191(11), 3451-3461.
15.Schembri, M. A., & Klemm, P. (1998). Heterobinary adhesins based on the Escherichia coli FimH fimbrial protein. Appl Environ Microbiol, 64(5), 1628-1633.
16.Sidhu, J. P., Ahmed, W., Hodgers, L., & Toze, S. (2013). Occurrence of virulence genes associated with diarrheagenic pathotypes in Escherichia coli isolates from surface water. Applied and environmental microbiology, 79(1), 328-335.
17.Tarchouna, M., Ferjani, A., Ben-Selma, W., & Boukadida, J. (2013). Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection. International Journal of Infectious Diseases, 17(6), e450-e453.
18.Thumbikat, P., Berry, R. E., Zhou, G., Billips, B. K., Yaggie, R. E., Zaichuk, T., . . . Klumpp, D. J. (2009). Bacteria-induced uroplakin signaling mediates bladder response to infection. PLoS Pathog, 5(5), 164-175.
19.Tiba, M. R., Yano, T., & Leite, D. d. S. (2008). Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis. Revista do Instituto de Medicina Tropical de Sao Paulo, 50(5), 255-260.
Infection Epidemiology and Microbiology
Volume 7, Issue 4
November 2021
Pages 297-304