Prevalence, Comparison of Diagnostic Methods, Antibiogram, and Genotyping of Arcobacter spp. in Diarrheal Cases Referring to Clinical Centers in Iran

Document Type : Original Research

Authors
S. Khalili Dermani
M. Akbari
M. Arjomandzadegan
M. Arjomandzadegan
A. Ahmadi
Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak,IR
10.52547/iem.8.2.107
Abstract
Backgrounds: Arcobacter is an emerging bacterium that may cause watery diarrhea and septicemia in humans. This study aimed to investigate the prevalence of Arcobacter spp. in diarrheal stool specimens using culture and molecular methods, their genetic diversity, and their resistance to different antibiotics in patients referring to clinical centers in Arak, Iran.

Materials & Methods: In this descriptive cross-sectional study, diarrheal stool specimens were collected from 230 patients over a two-month period from July to September 2016. The samples were tested for the presence of Arcobacter species. Suspected colonies were subjected to biochemical tests and identified by phenotypic methods. In addition, antimicrobial susceptibility testing was performed using the disk diffusion method. Arcobacter spp. were also directly detected by multiplex-PCR.

Findings: Out of 230 samples, 20 samples (8.69%) were positive in culture method, and 44 samples (19.13%) were positive in PCR method, all culture-positive samples were also positive in PCR method. Rep-PCR indicated 14 different rep types among Arcobacter spp. isolated from patients with gastroenteritis. All Arcobacter isolates were resistant to cefazolin, ceftazidime, and nalidixic acid. The isolates showed high susceptibility to tetracycline, gentamicin, ampicillin, amikacin, meropenem, erythromycin, and ciprofloxacin.

Conclusions: To the best of our knowledge, this is the first study conducted in Iran to isolate Arcobacter spp. from patients with gastroenteritis. The results indicate that Arcobacter spp. are one of the main causes of acute diarrhea in humans. The research outcomes show that Arcobacter spp. could be considered as the etiology of gastrointestinal infections in humans.

Keywords

Subjects

1.Debruyne L, Gevers D, Vandamme P. Taxonomy of the family Campylobacteraceae. Campylobacter, Third Edition: American Society of Microbiology; 2008. p. 3-25.
2. Jiang Z-D, DuPont HL, Brown EL, Nandy RK, Ramamurthy T, Sinha A, et al. Microbial etiology of travelers' diarrhea in Mexico, Guatemala, and India: importance of enterotoxigenic Bacteroides fragilis and Arcobacter species. Journal of clinical microbiology. 2010;48(4):1417-9.
3. Fernandez H, Villanueva MP, Mansilla I, Gonzalez M, Latif F. Arcobacter butzleri and A. cryaerophilus in human, animals and food sources, in southern Chile. Brazilian Journal of Microbiology. 2015;46(1):145-7.
4. Ramees TP, Dhama K, Karthik K, Rathore RS, Kumar A, Saminathan M, et al. Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control–a comprehensive review. Veterinary Quarterly. 2017;37(1):136-61.
5. Diéguez AL, Balboa S, Magnesen T, Romalde JL. Arcobacter lekithochrous sp. nov., isolated from a molluscan hatchery. International journal of systematic and evolutionary microbiology. 2017;67(5):1327-32.
6. Pérez-Cataluña A, Salas-Massó N, Figueras MJ. Arcobacter canalis sp. nov., isolated from a water canal contaminated with urban sewage. International journal of systematic and evolutionary microbiology. 2018;68(4):1258-64.
7. Lau S, Woo P, Teng J, Leung K, Yuen K. Identification by 16S ribosomal RNA gene sequencing of Arcobacter butzleri bacteraemia in a patient with acute gangrenous appendicitis. Journal of Clinical Pathology. 2002;55(3):182.
8. Samie A, Obi C, Barrett L, Powell S, Guerrant R. Prevalence of Campylobacter species, Helicobacter pylori and Arcobacter species in stool samples from the Venda region, Limpopo,
South Africa: studies using molecular diagnostic methods. Journal of Infection. 2007;54(6):558-66.
9. Woo PC, Chong KT, Leung K-w, Que T-l, Yuen K-y. Identification of Arcobacter cryaerophilus isolated from a traffic accident victim with bacteremia by 16S ribosomal RNA gene sequencing. Diagnostic microbiology and infectious disease. 2001;40(3):125-7.
10. Vandamme P, Vancanneyt M, Pot B, Mels L, Hoste B, Dewettinck D, et al. Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. International Journal of Systematic and Evolutionary Microbiology. 1992;42(3):344-56.
11. Van Driessche E, Houf K, Van Hoof J, De Zutter L, Vandamme P. Isolation of Arcobacter species from animal feces. FEMS microbiology letters. 2003;229(2):243-8.
12. Arguello E, Otto CC, Mead P, Babady NE. Bacteremia caused by Arcobacter butzleri in an immunocompromised host. Journal of clinical microbiology. 2015;53(4):1448-51.
13. Skovgaard N. Microorganisms in Foods 7: Microbiological Testing in Food Safety Management: By International Commission for the Microbiological Specifications of Foods (ICMSF). Kluwer Academic/Plenum Publishers, New York, xiii+ 367 pages, hardbound, ISBN 0-306-47262-7,€ 144.50/US $125.00/GP£ 88.50. http://www. wkap. nl. Elsevier; 2003.
14. Ferreira S, Queiroz JA, Oleastro M, Domingues FC. Insights in the pathogenesis and resistance of Arcobacter: a review. Critical reviews in microbiology. 2016;42(3):364-83.
15. Vandamme P, Falsen E, Rossau R, Hoste B, Segers P, Tytgat R, et al. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. International Journal of Systematic and Evolutionary Microbiology. 1991;41(1):88-103.
16. Prouzet-Mauléon V, Labadi L, Bouges N, Ménard A, Mégraud F. Arcobacter butzleri: underestimated enteropathogen. Emerging infectious diseases. 2006;12(2):307.
17. Ramees TP, Rathore RS, Bagalkot PS, Mohan HV, Kumar A, Dhama K. Detection of Arcobacter butzleri and Arcobacter cryaerophilus in clinical samples of humans and foods of animal origin by cultural and multiplex PCR based methods. Asian J Anim Vet Adv. 2014;9:243-52.
18. Fera MT, Russo GT, Di Benedetto A, La Camera E, Orlando A, Giandalia A, et al. High prevalence of Arcobacter carriage in older subjects with type 2 diabetes. BioMed Research International. 2010;2010.
19. Webb AL, Boras VF, Kruczkiewicz P, Selinger LB, Taboada EN, Inglis GD. Comparative detection and quantification of Arcobacter butzleri in stools from diarrheic and nondiarrheic people in Southwestern Alberta, Canada. Journal of clinical microbiology. 2016;54(4):1082-8.
20. Lastovica AJ, le Roux E. Efficient isolation of campylobacteria from stools. Journal of clinical microbiology. 2000;38(7):2798-9.
21. Taylor DN, Kiehlbauch JA, Tee W, Pitarangsi C, Echeverria P. Isolation of group 2 aerotolerant Campylobacter species from Thai children with diarrhea. Journal of Infectious Diseases. 1991;163(5):1062-7.
22. Mohan HV, Rathore RS, Dhama K, Ramees TP, Patyal A, Bagalkot P, et al. Prevalence of Arcobacter spp. in humans, animals and foods of animal origin in India based on cultural
isolation, antibiogram, PCR and multiplex PCR detection. Asian J Anim Vet Adv. 2014;9(8):452-66.
23. Mandisodza O, Burrows E, Nulsen M. Arcobacter species in diarrhoeal faeces from humans in New Zealand. The New Zealand Medical Journal (Online). 2012;125(1353).
24. Kayman T, Abay S, Hizlisoy H, Atabay HI, Diker KS, Aydin F. Emerging pathogen Arcobacter spp. in acute gastroenteritis: molecular identification, antibiotic susceptibilities and genotyping of the isolated arcobacters. Journal of medical microbiology. 2012;61(10):1439-44.
25. Hsueh P-R, Teng L-J, Yang P-C, Wang S-K, Chang S-C, Ho S-W, et al. Bacteremia caused by Arcobacter cryaerophilus 1B. Journal of clinical microbiology. 1997;35(2):489-91.
26. Atabay HI, Wainø M, Madsen M. Detection and diversity of various Arcobacter species in Danish poultry. International journal of food microbiology. 2006;109(1-2):139-45.
27. Pejchalova M, Zabcikova S, Silhova L, Silha D, Brozkova I, Haslova M. Presence of Arcobacter species in pet cats and dogs in the Czech Republic. Veterinarni Medicina. 2016;61(8).
28. Merga J, Leatherbarrow A, Winstanley C, Bennett M, Hart C, Miller W, et al. Comparison of Arcobacter isolation methods, and diversity of Arcobacter spp. in Cheshire, United Kingdom. Applied and environmental microbiology. 2011;77(5):1646-50.
29. Vandenberg O, Dediste A, Houf K, Ibekwem S, Souayah H, Cadranel S, et al. Arcobacter species in humans. Emerging infectious diseases. 2004;10(10):1863
30. Houf K, De Zutter L, Van Hoof J, Vandamme P. Assessment of the genetic diversity among arcobacters isolated from poultry products by using two PCR-based typing methods. Applied and environmental microbiology. 2002;68(5):2172-8.
31. Van Driessche E, Houf K, Vangroenweghe F, De Zutter L, Van Hoof J. Prevalence, enumeration and strain variation of Arcobacter species in the faeces of healthy cattle in Belgium. Veterinary microbiology. 2005;105(2):149-54.
32. Aydin F, Gümüşsoy K, Atabay H, Ica T, Abay S. Prevalence and distribution of Arcobacter species in various sources in Turkey and molecular analysis of isolated strains by ERIC‐PCR. Journal of applied microbiology. 2007;103(1):27-35.
33. Phasipol P, Chokesajjawatee N, Luangtongkum T. Comparison of repetitive sequence-based polymerase chain reaction (rep-PCR) and pulsed-field gel electrophoresis (PFGE) for genetic characterization of Arcobacter spp. The Thai Journal of Veterinary Medicine. 2013;43(2):307.
34. Ramees TP, Rathore RS, Bagalkot PS, Sailo B, Mohan HV, Kumar A, et al. Genotyping and genetic diversity of Arcobacter butzleri and Arcobacter cryaerophilus isolated from different sources by using ERIC-PCR from India. Veterinary Quarterly. 2014;34(4):211-7.
35. Sekhar MS, Rao TS, Chinnam B, Subramanyam K, Metta M, Sharif NM. Genetic diversity of Arcobacter species of animal and human origin in Andhra Pradesh, India. Indian journal of microbiology. 2017;57(2):250-2.
36. Bronzwaer S. Harmonised monitoring of antimicrobial resistance in Salmonella and Campylobacter isolates from food animals in the European :union:. Clinical microbiology and infection. 2008;14(6):522-33.
37. Ferreira S, Luis A, Oleastro M, Pereira L, Domingues F. A meta-analytic perspective on Arcobacter spp. antibiotic resistance. Journal of global antimicrobial resistance. 2019.
38. Pérez-Cataluña A, Tapiol J, Benavent C, Sarvisé C, Gomez F, Martinez B, et al. Antimicrobial susceptibility, virulence potential and sequence types associated with Arcobacter strains recovered from human faeces. Journal of medical microbiology. 2017;66(12):1736-43.
39. Vandenberg O, Houf K, Douat N, Vlaes L, Retore P, Butzler J-P, et al. Antimicrobial susceptibility of clinical isolates of non-jejuni/coli campylobacters and arcobacters from Belgium. Journal of antimicrobial chemotherapy. 2006;57(5):908-13.
40. Van den Abeele A-M, Vogelaers D, Vanlaere E, Houf K. Antimicrobial susceptibility testing of Arcobacter butzleri and Arcobacter cryaerophilus strains isolated from Belgian patients. Journal of Antimicrobial Chemotherapy. 2016;71(5):1241-4.
41. Aski HS, Tabatabaei M, Khoshbakht R, Raeisi M. Occurrence and antimicrobial resistance of emergent Arcobacter spp. isolated from cattle and sheep in Iran. Comparative immunology, microbiology and infectious diseases. 2016;44:37-40.
Infection Epidemiology and Microbiology
Volume 8, Issue 2
June 2022
Pages 107-119