Evaluation of Anticancer Potential of Silver Chloride Nanoparticles Biosynthesized by Penicillium chrysogenum

Document Type : Original Research

Authors
S. Zamani 1
M. Fazilati 1
M. Hadian 2
H. Nazem 1
N. Noohi 2
1 Department of Biochemistry, Payame Noor University, Isfahan, Iran
2 Research Center for Conservation of Cultural Relics, Research Institute of cultural heritage & tourism, Tehran, Iran
10.52547/iem.8.2.159
Abstract
Backgrounds: Green synthesis of nanoparticles (NPs) is a simple, fast, and eco-friendly method which could be performed by various microorganisms or plant extracts. Silver NPs are well-known as antimicrobial and anti-fungal materials. They play an essential role in the control of tumors via their cytotoxic effects. Therefore, they have attracted significant attention for developing an effective treatment solution for cancer cells. This study aimed to investigate the potential of Penicillium chrysogenum for the synthesis of silver NPs and to evaluate their toxicity on liver cancer cell line (HepG2).

Materials & Methods: After synthesis of NPs usingP. chrysogenum, characterization of the synthesized NPs was performed by UV–Vis spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was carried out to detect biomolecules that may be responsible for the synthesis and stabilization of NPs. The cytotoxic activity of the synthesized AgclNPs on HepG2 cell line was evaluated using MTT assay.

Findings: UV–Vis spectroscopy and XRD analysis confirmed the synthesis of AgclNPs using P. chrysogenum. TEM analysis revealed the spherical shape of AgclNPs with an average crystalline size of 15 to 45 nm. FTIR spectroscopy indicated the possible functional groups that could be responsible for the reduction of metal ions and the capping process. These nanoparticles showed a dose-dependent anticancer activity against HepG2 cells.

Conclusion: The results suggest that biosynthesized silver chloride nanoparticles could offer potential applications in cancer therapy.

Keywords

Subjects

1. Andleeb A, Andleeb A, Asghar S, Zaman G, Tariq M, Mehmood A, et al. A Systematic Review of Biosynthesized Metallic Nanoparticles as a Promising Anti-Cancer-Strategy. Cancers. 2021;13(11):2818.
2. Ratan ZA, Haidere MF, Nurunnabi M, Shahriar SM, Ahammad A, Shim YY, et al. Green chemistry synthesis of silver nanoparticles and their potential anticancer effects. Cancers. 2020;12(4):855.
3. Keat CL, Aziz A, Eid AM, Elmarzugi NA. Biosynthesis of nanoparticles and silver nanoparticles. Bioresources and Bioprocessing. 2015;2(1):47.
4. Rafique M, Sadaf I, Rafique MS, Tahir MB. A review on green synthesis of silver nanoparticles and their applications. Artificial cells, nanomedicine, and biotechnology. 2017;45(7):1272-91.
5. Ahmad S, Munir S, Zeb N, Ullah A, Khan B, Ali J, et al. Green nanotechnology: a review on green synthesis of silver nanoparticles - an ecofriendly approach. International Journal of Nanomedicine. 2019;14:5087-107.
6. Jeyaraj M, Sathishkumar G, Sivanandhan G, MubarakAli D, Rajesh M, Arun R, et al. Biogenic silver nanoparticles for cancer treatment: An experimental report. Colloids and Surfaces B: Biointerfaces. 2013;106:86-92.
7. Chugh H, Sood D, Chandra I, Tomar V, Dhawan G, Chandra R. Role of gold and silver nanoparticles in cancer nano-medicine. Artificial cells, nanomedicine, and biotechnology. 2018;46(sup1):1210-20.
8. Saratale RG, Shin HS, Kumar G, Benelli G, Kim D-S, Saratale GD. Exploiting antidiabetic activity of silver nanoparticles synthesized using Punica granatum leaves and anticancer potential against human liver cancer cells (HepG2). Artificial cells, nanomedicine, and biotechnology. 2018;46(1):211-22.
9. He Y, Li X, Zheng Y, Wang Z, Ma Z, Yang Q, et al. A green approach for synthesizing silver nanoparticles, and their antibacterial and cytotoxic activities. New Journal of Chemistry. 2018;42(4):2882-8.
10. Xu Z, Feng Q, Wang M, Zhao H, Lin Y, Zhou S. Green Biosynthesized Silver Nanoparticles With Aqueous Extracts of Ginkgo Biloba Induce Apoptosis via Mitochondrial Pathway in Cervical Cancer Cells. Frontiers in oncology. 2020;10:2282.
11. Adebayo IA, Arsad H, Gagman HA, Ismail NZ, Samian MR. Inhibitory Effect of Eco-Friendly Naturally Synthesized Silver Nanoparticles from the Leaf Extract of Medicinal Detarium microcarpum Plant on Pancreatic and Cervical Cancer Cells. Asian Pacific Journal of Cancer Prevention. 2020;21(5):1247-52.
12. Nayaka S, Chakraborty B, Pallavi S, Bhat MP, Shashiraj K, Ghasti B. Synthesis of biogenic silver nanoparticles using Zanthoxylum rhetsa (Roxb) DC seed coat extract as reducing agent and in-vitro assessment of anticancer effect on A549 lung cancer cell line. International Journal of Pharmaceutical Research. 2020;12(3):302-14.
13. Annu M, Ahmed S, Kaur G, Sharma P, Singh S, Ikram S. Evaluation of the antioxidant, antibacterial and anticancer (lung cancer cell line A549) activity of Punica granatum mediated silver nanoparticles. Toxicology Research. 2018;7(5):923-30.
14. Venkatadri B, Shanparvish E, Rameshkumar MR, Arasu MV, Al-Dhabi NA, Ponnusamy VK, et al. Green synthesis of silver nanoparticles using aqueous rhizome extract of Zingiber officinale and Curcuma longa: In-vitro anti-cancer potential on human colon carcinoma HT-29 cells. Saudi Journal of Biological Sciences. 2020;27(11):2980-6.
15. Huang F, Long Y, Liang Q, Purushotham B, Swamy MK, Duan Y. Safed Musli Chlorophytum borivilianum L.) Callus-Mediated Biosynthesis of Silver Nanoparticles and Evaluation of their Antimicrobial Activity and Cytotoxicity against Human Colon Cancer Cells. Journal of Nanomaterials. 2019;2019:2418785.
16. Elgamouz A, Idriss H, Nassab C, Bihi A, Bajou K, Hasan K, et al. Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles. Nanomaterials. 2020;10(9):1861.
17. Iqbal MJ, Ali S, Rashid U, Kamran M, Malik MF, Sughra K, et al. Biosynthesis of silver nanoparticles from leaf extract of Litchi chinensis and its dynamic biological impact on microbial cells and human cancer cell lines. Cellular and Molecular Biology. 2018;64(13):42-7.
18. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications. 1990;18(1):315-22.
19. Sadowski Z, Maliszewska I, Grochowalska B, Polowczyk I, Kozlecki T. Synthesis of silver nanoparticles using microorganisms. Materials Science-Poland. 2008;26(2):419-24.
20. Pourali P, Yahyaei B. Biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat. Journal of Trace Elements in Medicine and Biology. 2016;34:22-31.
21. Prabhu D, Arulvasu C, Babu G, Manikandan R, Srinivasan P. Biologically synthesized green silver nanoparticles from leaf extract of Vitex negundo L. induce growth-inhibitory effect on human colon cancer cell line HCT15. Process Biochemistry. 2013;48(2):317-24.
22. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983;65(1):55-63.
23. Jeffery JW. Methods in X-ray Crystallography. 1971.
24. Durán N, Marcato PD, Alves OL, De Souza GIH, Esposito E. Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. Journal of Nanobiotechnology. 2005;3(1):8.
25. Naqvi SZH, Kiran U, Ali MI, Jamal A, Hameed A, Ahmed S, et al. Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria. International Journal of Nanomedicine. 2013;8:3187-95.
26. Lotfy WA, Alkersh BM, Sabry SA, Ghozlan HA. Biosynthesis of Silver Nanoparticles by Aspergillus terreus: Characterization, Optimization, and Biological Activities. Frontiers in Bioengineering and Biotechnology. 2021;9(265).
27. Bhainsa KC, D'Souza SF. Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. Colloids and Surfaces B: Biointerfaces. 2006;47(2):160-4.
28. Balaji DS, Basavaraja S, Deshpande R, Mahesh DB, Prabhakar BK, Venkataraman A. Extracellular biosynthesis of functionalized silver nanoparticles by strains of Cladosporium cladosporioides fungus. Colloids and Surfaces B: Biointerfaces. 2009;68(1):88-92.
29. Kathiresan K, Manivannan S, Nabeel MA, Dhivya B. Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment. Colloids and Surfaces B: Biointerfaces. 2009;71(1):133-7.
30. Taha ZK, Hawar SN, Sulaiman GM. Extracellular biosynthesis of silver nanoparticles from Penicillium italicum and its antioxidant, antimicrobial and cytotoxicity activities. Biotechnology Letters. 2019;41(8):899-914.
31. Sastry M, Mayya KS, Bandyopadhyay K. pH Dependent changes in the optical properties of carboxylic acid derivatized silver colloidal particles. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 1997;127(1):221-8.
32. Huo Y, Han YX, Singh P, Kang JP, Pu JY, Piao CH, et al. Antimicrobial, antioxidant, and anticancer potentials of AgCl nanoparticles biosynthesized by Flavobacterium panacis. Applied Physics A. 2021;127(4):227.
33. Rasulov BA, Pattaeva MA, Li W-J. Controlled biosynthesis of AgCl nanoparticles by a thermotolerant Aspergillus terreus in the L-Tryptophan supplemented media: Characterization and antimicrobial activity. Microbiology. 2017;86(4):517-23.
34. Al Aboody MS. Silver/silver chloride (Ag/AgCl) nanoparticles synthesized from Azadirachta indica lalex and its antibiofilm activity against fluconazole resistant Candida tropicalis. Artificial Cells, Nanomedicine, and Biotechnology. 2019;47(1):2107-13.
35. Jain N, Bhargava A, Majumdar S, Tarafdar J, Panwar J. Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: a mechanism perspective. Nanoscale. 2011;3(2):635-41.
36. Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, et al. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids and Surfaces B: Biointerfaces. 2003;28(4):313-8.
37. Rauwel P, Küünal S, Ferdov S, Rauwel E. A Review on the Green Synthesis of Silver Nanoparticles and Their Morphologies Studied via TEM. Advances in Materials Science and Engineering. 2015;2015:682749.
38. Patra JK, Baek K-H. Green synthesis of silver chloride nanoparticles using Prunus persica L. outer peel extract and investigation of antibacterial, anticandidal, antioxidant potential. Green Chemistry Letters and Reviews. 2016;9(2):132-42.
39. Valery PC, Laversanne M, Clark PJ, Petrick JL, McGlynn KA, Bray F. Projections of primary liver cancer to 2030 in 30 countries worldwide. Hepatology. 2018;67(2):600-11.
40. Al-Khedhairy AA, Wahab R. Silver Nanoparticles: An Instantaneous Solution for Anticancer Activity against Human Liver (HepG2) and Breast (MCF-7) Cancer Cells. Metals. 2022;12(1).
41. Ahmadian E, Dizaj SM, Rahimpour E, Hasanzadeh A, Eftekhari A, Hosain zadegan H, et al. Effect of silver nanoparticles in the induction of apoptosis on human hepatocellular carcinoma (HepG2) cell line. Materials Science and Engineering: C. 2018;93:465-71.
42. Faedmaleki F, H Shirazi F, Salarian A-A, Ahmadi Ashtiani H, Rastegar H. Toxicity Effect of Silver Nanoparticles on Mice Liver Primary Cell Culture and HepG2 Cell Line. Iranian Journal of Pharmaceutical Research. 2014;13(1):235-42.
Infection Epidemiology and Microbiology
Volume 8, Issue 2
June 2022
Pages 159-167