Mucosa-Associated Escherichia coli Producing Cyclomodulin toxins in Colon Cancer and intestinal inflammatory Patients

Ghiasvand, Sima; Saidijam, Massoud; Roshanaei, Ghodratollah; Jafari, Mohammad; Taheri, Mohammad; Alikhani, Mohammad Sina; Kazemi, Sima; Alikhani, Mohammad Yousef

doi:10.52547/iem.11.4.317

Mucosa-Associated Escherichia coli Producing Cyclomodulin toxins in Colon Cancer and intestinal inflammatory Patients

Document Type : Original Article

Authors

Sima Ghiasvand ¹

Massoud saidijam ²

ghodratollah roshanaei ³

Mohammad Jafari ⁴

Mohammad Taheri ¹

Mohammad Sina Alikhani ⁵

sima kazemi ⁶

Mohammad Yousef Alikhani ⁶

¹ Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

² Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran

³ Modeling of Non communicable Diseases Research Center, Institute of Health Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, Iran

⁴ Department of Pathology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

⁵ Student research center, Hamadan University of Medical Sciences, Hamadan, Iran

⁶ Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran

10.52547/iem.11.4.317

Abstract

Background: Colorectal cancer (CRC) is the third most common cause of death. In the human intestinal tract, some Escherichia coli strains produce cyclomodulin toxins. This study aimed to determine the frequency of cyclomodulin-encoding genes in E. coli isolates from patients with CRC and inflammatory bowel disease (IBD) compared to healthy subjects.
Materials & Methods: A total of 120 E. coli strains were isolated from colonic mucosa samples during 2016-2017 from Hamadan, Iran. E. coli isolates were identified using biochemical tests. Phylogroups of E. coli isolates and cyclomodulin toxin-encoding genes were identified by PCR. The results were analyzed by SPSS software.
Findings: The predominant E. coli phylogroups were A (52.5%), B2 (52.5%), and A (55%) in the CRC, IBD, and healthy groups, respectively. E. coli isolates harboring the pks (32.5%) and cnf1 (27.5%) genes belonged to phylogroup B2 (p< .001), and isolates harboring the pks gene were more prevalent in CRC patients (30%). The cnf3 gene had the highest frequency (30.8%) in the cnf gene family. The highest prevalence of cnf1 (27.5%) was observed in E. coli phylogroup B2, the highest prevalence of cdt4 (58.3%) was in phylogroup B1, and the highest prevalence of cif (52.5%) was strongly related to phylogroup B2.
Conclusion: The presence of cyclomodulin toxin-encoding genes in E. coli isolates was not associated with CRC, and no statistical difference was observed in the level of cyclomodulin-encoding genes in E. coli isolates. On the other hand, there is no information about the critical time of host-microbe interaction for tumorigenesis.

Keywords

E. coli: Escherichia coli

Colorectal

Cancer

Cyclomodulin

PCR

Subjects