Volume 4, Issue 1 (2018)
IEM 2018, 4(1): 19-25 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Motamedi H, Rasoolizadeh A, Shahshahan Z, Razavi V. Prevalence of Chlamydia trachomatis Infection in Cervical Samples from Infertile Women and Related Genotypes. IEM 2018; 4 (1) :19-25
URL: http://iem.modares.ac.ir/article-4-13952-en.html
URL: http://iem.modares.ac.ir/article-4-13952-en.html
1- “Biotechnology & Biological Science Research Center” and “Biology Department, Science Faculty”, Shahid Chamran University of Ahvaz, Ahvaz, Iran , hhmotamedi@yahoo.com
2- Biology Department, Science Faculty, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3- Obstetrics & Gynecology Department, Medicine Faculty, Isfahan University of Medical Science, Isfahan, Iran
4- Shahid Beheshti Hospital, Isfahan, Iran
2- Biology Department, Science Faculty, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3- Obstetrics & Gynecology Department, Medicine Faculty, Isfahan University of Medical Science, Isfahan, Iran
4- Shahid Beheshti Hospital, Isfahan, Iran
Abstract: (5659 Views)
Background: Chlamydia trachomatis infection is a sexually transmitted disease that its majority infections are asymptomatic and can cause infertility. So, determining its frequency and prevalent genotypes in each zone is necessary to provide clues for clinicians and also to prevent or minimize its complications. The aim of this study was determining the prevalence of C. trachomatis and related genotypes in infertile women and relation with type of infertility.
Methods: For this purpose, a primary screening based on amplification of cryptic plasmid gene was done on endocervical samples obtained from infertile women referring to two infertility treatment clinics. Genotyping of positive samples was done based on PCR-RFLP of omp1 gene and then DNA digestion with Hpa II, Hinf I and Alu I restriction enzymes. The association of infection with age, abortion and primary and secondary infertility was analyzed by statistical analysis.
Results: As a result of this study, the frequency of C. trachomatis infection in 180 endocervical samples was 10.5 % in infertile women. The PCR-RFLP analysis results showed that E, F and D genotypes are prevalent in this population. There was a significant association between infection and abortion among patients with primary and secondary infertility.
Conclusions: Finally, based on the obtained results it can be concluded that C. trachomatis infection is prevalent in infertile women especially in secondary infertility. So, it must be regarded in preventive care and control program hence its diagnosis and treatment can reduce infertility rate among women.
Methods: For this purpose, a primary screening based on amplification of cryptic plasmid gene was done on endocervical samples obtained from infertile women referring to two infertility treatment clinics. Genotyping of positive samples was done based on PCR-RFLP of omp1 gene and then DNA digestion with Hpa II, Hinf I and Alu I restriction enzymes. The association of infection with age, abortion and primary and secondary infertility was analyzed by statistical analysis.
Results: As a result of this study, the frequency of C. trachomatis infection in 180 endocervical samples was 10.5 % in infertile women. The PCR-RFLP analysis results showed that E, F and D genotypes are prevalent in this population. There was a significant association between infection and abortion among patients with primary and secondary infertility.
Conclusions: Finally, based on the obtained results it can be concluded that C. trachomatis infection is prevalent in infertile women especially in secondary infertility. So, it must be regarded in preventive care and control program hence its diagnosis and treatment can reduce infertility rate among women.
Subject:
Bacteriology
Received: 2018/01/19 | Accepted: 2018/05/27 | Published: 2018/05/27
Received: 2018/01/19 | Accepted: 2018/05/27 | Published: 2018/05/27
References
1. Abdelrahman YM, Belland RJ. The chlamydial developmental cycle. FEMS Microbiol Rev. 2005;29(5):949-59. [Link]
2. World Health Organization. Global prevalence and incidence of selected curable sexually transmitted infection, overview and estimates [Internet]. Geneva: World Health Organization; 2001 [cited 2016 May 12]. Available from: http://apps.who.int/iris/handle/10665/66818. [Link]
3. Castellsagué X, Peeling RW, Franceschi S, de Sanjosé S, Smith JS, Albero G, et al. Chlamydia trachomatis infection in female partners of circumcised and uncircumcised adult men. Am J Epidemiol. 2005;162(9):907-16. [Link]
4. Simonetti AC, Melo JH, De Souza PR, Bruneska D, De Lima Filho JL. Immunological's host profile for HPV and Chlamydia trachomatis, a cervical cancer cofactor. Microbes Infect. 2009;11(4):435-42. [Link]
5. Hsu MC, Tsai PY, Chen KT, Li LH, Chiang CC, Tsai JJ, et al. Genotyping of Chlamydia trachomatis from clinical specimens in Taiwan. J Med Microbiol. 2006;55(Pt 3):301-8. [Link]
6. Ngandjio A, Clerc M, Fonkoua MC, Thonnon J, Lunel F, Bébéar C, et al. Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students. J Med Microbiol. 2004;53(Pt 1):47-50. [Link]
7. Morré SA, Ossewaarde JM, Lan J, Van Doornum GJ, Walboomers JM, Maclaren DM, et al. Serotyping and genotyping of genital Chlamydia trachomatis isolates reveal variants of serovars Ba, G, and J as confirmed by omp1 nucleotide sequence analysis. J Clin Microbiol. 1998;36(2):345-51. [Link]
8. Sturm-Ramirez K, Brumblay H, Diop K, Guèye-Ndiaye A, Sankalé JL, Thior I, et al. Molecular epidemiology of genital Chlamydia trachomatis infection in high-risk women in Senegal, West Africa. J Clin Microbiol. 2000;38(1):138-45. [Link]
9. Yuan Y, Zhang YX, Watkins NG, Caldwell HD. Nucleotide and deduced amino acid sequences for the four variable domains of the major outer membrane proteins of the 15 Chlamydia trachomatis serovars. Infect Immun. 1989;57(4):1040-9. [Link]
10. Spauwen LW, Hoebe CJ, Brouwers EE, Dukers-Muijrers NH. Improving STD testing behavior among high-risk young adults by offering STD testing at a vocational school. BMC Public Health. 2011;11:750. [Link]
11. Sweet RL. Treatment of acute pelvic inflammatory disease. Infect Dis Obstet Gynecol. 2011;2011:561909. [Link]
12. Wiesenfeld HC, Hillier SL, Krohn MA, Amortegui AJ, Heine RP, Landers DV, et al. Lower genital tract infection and endometritis: Insight into subclinical pelvic inflammatory disease. Obstet Gynecol. 2002;100(3):456-63. [Link]
13. Hosseinzadeh S, Pacey AA, Eley A. Chlamydia trachomatis-induced death of human spermatozoa is caused primarily by lipopolysaccharide. J Med Microbiol. 2003;52(3):193-200. [Link]
14. Morré SA, Rozendaal L, van Valkengoed IG, Boeke AJ, Van Voorst Vader PC, Schirm J, et al. Urogenital Chlamydia trachomatis serovars in men and women with a symptomatic or asymptomatic infection: An association with clinical manifestations?. J Clin Microbiol. 2000;38(6):2292-6. [Link]
15. Pavletic AJ, Wölner-Hanssen P, Paavonen J, Hawes SE, Eshenbach DA. Infertility following pelvic inflammatory disease. Infect Dis Obstet Gynecol. 1999;7(3):145-52. [Link]
16. Bébéar C, De Barbeyrac B. Genital Chlamydia trachomatis infections. Clin Microbiol Infect. 2009;15(1):4-10. [Link]
17. Lan J, Walboomers JM, Roosendaal R, Van Doornum GJ, MacLaren DM, Meijer CJ, et al. Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis. J Clin Microbiol. 1993;31(5):1060-5. [Link]
18. Rodriguez P, Vekris A, De Barbeyrac B, Dutilh B, Bonnet J, Bebear C. Typing of Chlamydia trachomatis by restriction endonuclease analysis of the amplified major outer membrane protein gene. J Clin Microbiol. 1991;29(6):1132-6. [Link]
19. Taheri Beni B, Motamedi H, Ardakani MR. Genotyping of the prevalent Chlamydia trachomatis strains involved in cervical infections in women in Ahvaz, Iran. J Med Microbiol. 2010;59(Pt 9):1023-8. [Link]
20. Carroll KC, Aldeen WE, Morrision M, Anderson R, Lee D, Mottice S. Evaluation of the Abbott LCx ligase chain reaction assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine and genital swab specimens from a sexually transmitted disease clinic population. J Clin Microbiol. 1998;36(6):1630-3. [Link]
21. Shafer MA, Moncada J, Boyer CB, Betsinger K, Flinn SD, Schachter J. Comparing first-void urine specimens, self-collected vaginal swabs, and endocervical specimens to detect Chlamydia trachomatis and Neisseria gonorrhoeae by a nucleic acid amplification test. J Clin Microbiol. 2003;41(9):4395-9. [Link]
22. Jenab A, Roghanian R, Golbang N, Golbang P, Chammani Tabriz L. Comparison of three methods of DNA extraction in endocervical specimens for Chlamydia trachomatis infection by spectrophotometry, agarose gel, and PCR. Arch Immunol Ther Exp (Warsz). 2010;58(3):227-34. [Link]
23. Siala M, Gdoura R, Younes M, Fourati H, Cheour I, Meddeb N, et al. Detection and frequency of Chlamydia trachomatis DNA in synovial samples from Tunisian patients with reactive arthritis and undifferentiated oligoarthritis. FEMS Immunol Med Microbiol. 2009;55(2):178-86. [Link]
24. Zeighami H, Sattari M, , Najar Peerayeh S, Mota A. Detection of Chlamydia trachomatis in endocervical smears of women with abortion by PCR. Res J Biol Sci. 2008;3(3):342-4. [Link]
25. Chamani Tabriz L, Tehrani MJ, Akhondi MM, Mosavi Jarrahi A, Zeraati H, Ghasemi J, et al. Chlamydia trachomatis prevalence in Iranian women attending obstetrics and gynaecology clinics. Pak J Biol Sci. 2007;10(24):4490-4. [Link]
26. Fallah F, Kazemi B, Goudarzi H, Badami N, Doostdar F, Ehteda A, et al. Detection of Chlamydia trachomatis from urine specimens by PCR in women with cervicitis. Iran J Public Health. 2005;34(2):20-6. [Link]
27. Flipp E, Niemiec KT, Kowalska B, Pawlowska A, Kwiatkowska M. Chlamydia trachomatis infection in sexually active teenagers. Ginekol Pol. 2008;79(4):264-70. [Polish] [Link]
28. Domeika M, Hallen A, Drulyte O. Genital Chlamydia trachomatis infections in Lithuanian women invited for screening via newspaper advertisement: A pilot study. Sex Transm Infect. 2000;76(3):216. [Link]
29. Domeika M, Hallén A, Karabanov L, Chudomirova K, Gruber F, Unzeitig V, et al. Chlamydia trachomatis infection in eastern Europe: Legal aspects, epidemiology, diagnosis and treatment. Sex Transm Infect. 2002;78(2):115-9. [Link]
30. Levidiotou S, Vrioni G, Papadogeorgaki H, Avdeliodi K, Kada H, Kaparos G, et al. Chlamydia trachomatis infections in Greece: First prevalence study using nucleic acid amplification tests. Eur J Clin Microbiol Infect Dis. 2005;24(3):207-13. [Link]
31. Chen XS, Yin YP, Chen LP, Thuy NT, Zhang GY, Shi MQ, et al. Sexually transmitted infections among pregnant women attending an antenatal clinic in Fuzhou, China. Sex Transm Dis. 2006;33(5):296-301. [Link]
32. Mascellino MT, Ciardi MR, Oliva A, Cecinato F, Hassemer MP, Borqese L. Chlamydia trachomatis detection in a population of asympatomatic and symptomatic women: Correlation with the presence of serological markers for this infection. New Microbiol. 2008;31(2):249-56. [Link]
33. Van Bergen J, Götz HM, Richardus JH, Hoebe CJ, Broer J, Coenen AJ, et al. Prevalence of urogenital Chlamydia trachomatis increases significantly with level of urbanization and suggests targeted screening approaches: Results from the first national population based study in the Netherlands. Sex Transm Infect. 2005;81(1):17-23. [Link]
34. Patel A, Rashid S, Godfrey EM, Panchal H. Prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae genital infections in a publicly funded pregnancy termination clinic: Empiric vs. indicated treatment?. Contraception. 2008;78(4):328-31. [Link]
35. Malik A, Jain S, Hakim S, Shukla I, Rizvi M. Chlamydia trachomatis infection & female infertility. Indian J Med Res. 2006;123(6):770-5. [Link]
36. Persson K, Osser S. Lack of evidence of a relationship between genital symptoms, cervicitis and salpingitis and different serovar of Chlamydia trachomatis. Eur J Clin Microbiol Infect Dis. 1993;12(3):195-9. [Link]
37. Morré SA, Karimi O, Ouburg S. Chlamydia trachomatis: Identification of susceptibility markers for ocular and sexually transmitted infection by immunogenetics. FEMS Immunol Med Microbiol. 2009;55(2):140-53. [Link]
38. Van Duynhoven YT, Ossewaarde JM, Derksen-Nawrocki RP, Van Der Meijden WI, Van De Laar MJ. Chlamydia trachomatis genotypes: Correlation with clinical manifestations of infection and patients' characteristics. Clin Infect Dis. 1998;26(2):314-22. [Link]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |