Volume 4, Issue 4 (2018)                   IEM 2018, 4(4): 153-157 | Back to browse issues page

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1- Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran
2- Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran , mallahi@ut.ac.ir
3- Hepatitis & AIDS Departments, Pasteur Institute of Iran, Tehran, Iran
4- Persian Gulf & Oman Sea Ecological Research Institute, Bandar Abbas, Hormozgan, Iran
Abstract:   (2319 Views)
Aims: Antiviral activity and effect of methanol and diethyl ether extracts from different parts of sea cucumber (Holothuria leucospilota) against HIV-1 were assessed on human oral epidermoid carcinoma cells (KB) and Human embryonic kidney 293T cells (HEK293T).
Materials & Methods: Sea cucumber was collected at a depth of 10-30 m (Persian Gulf). Extracts were prepared by diethyl ether and methanol solvents. The antiviral activity of each extract was evaluated by inhibition of single-cycle HIV-1 (SCR HIV-1) p24 Core antigen production in HeLa cells and cellular toxicity of different extracts were assessed, using a cell proliferation XTT kit.
Findings: Antiviral activity of each extract showed that some concentrations were able to inhibit the replication of HIV-1. Diethyl ether extract of body wall with 2.79 TI index displayed the highest antiviral activity as well as less effect.
Conclusion: This study showed that crude extracts of Holothuria leucospilota, especially methanol and diethyl ether extracts of digestive organs and body wall and antiviral activity, respectively.
Full-Text [PDF 615 kb]   (603 Downloads)    
Article Type: Original Research | Subject: Virology
Received: 2018/05/27 | Accepted: 2018/10/8 | Published: 2018/12/20

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