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IEM 2018, 4(4): 153-157 |
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Bahroudi S, Nematollahi M, Aghasadeghi M, Nazemi M. In vitro Evaluation of the Antiviral Activity and Cytotoxicity Effect of Holothuria leucospilota Sea Cucumber Extracts from the Persian Gulf. IEM 2018; 4 (4) :153-157
URL: http://iem.modares.ac.ir/article-4-26525-en.html
URL: http://iem.modares.ac.ir/article-4-26525-en.html
1- Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran
2- Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran ,mallahi@ut.ac.ir
3- Hepatitis & AIDS Departments, Pasteur Institute of Iran, Tehran, Iran
4- Persian Gulf & Oman Sea Ecological Research Institute, Bandar Abbas, Hormozgan, Iran
2- Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran ,
3- Hepatitis & AIDS Departments, Pasteur Institute of Iran, Tehran, Iran
4- Persian Gulf & Oman Sea Ecological Research Institute, Bandar Abbas, Hormozgan, Iran
Abstract: (3350 Views)
Aims: Antiviral activity and effect of methanol and diethyl ether extracts from different parts of sea cucumber (Holothuria leucospilota) against HIV-1 were assessed on human oral epidermoid carcinoma cells (KB) and Human embryonic kidney 293T cells (HEK293T).
Materials & Methods: Sea cucumber was collected at a depth of 10-30 m (Persian Gulf). Extracts were prepared by diethyl ether and methanol solvents. The antiviral activity of each extract was evaluated by inhibition of single-cycle HIV-1 (SCR HIV-1) p24 Core antigen production in HeLa cells and cellular toxicity of different extracts were assessed, using a cell proliferation XTT kit.
Findings: Antiviral activity of each extract showed that some concentrations were able to inhibit the replication of HIV-1. Diethyl ether extract of body wall with 2.79 TI index displayed the highest antiviral activity as well as less effect.
Conclusion: This study showed that crude extracts of Holothuria leucospilota, especially methanol and diethyl ether extracts of digestive organs and body wall and antiviral activity, respectively.
Materials & Methods: Sea cucumber was collected at a depth of 10-30 m (Persian Gulf). Extracts were prepared by diethyl ether and methanol solvents. The antiviral activity of each extract was evaluated by inhibition of single-cycle HIV-1 (SCR HIV-1) p24 Core antigen production in HeLa cells and cellular toxicity of different extracts were assessed, using a cell proliferation XTT kit.
Findings: Antiviral activity of each extract showed that some concentrations were able to inhibit the replication of HIV-1. Diethyl ether extract of body wall with 2.79 TI index displayed the highest antiviral activity as well as less effect.
Conclusion: This study showed that crude extracts of Holothuria leucospilota, especially methanol and diethyl ether extracts of digestive organs and body wall and antiviral activity, respectively.
Article Type: Original Research |
Subject:
Virology
Received: 2018/05/27 | Accepted: 2018/10/8 | Published: 2018/12/20
Received: 2018/05/27 | Accepted: 2018/10/8 | Published: 2018/12/20
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