Volume 4, Issue 4 (2018)                   IEM 2018, 4(4): 0-0 | Back to browse issues page

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hajikhani S, Darban-Sarokhalil D, Babapour E. Antibacterial susceptibility profiles and the presence of beta-lactamase resistance genes and virulence factors in Pseudomonas aeruginosa isolates . IEM. 2018; 4 (4)
URL: http://journals.modares.ac.ir/article-4-28097-en.html
1- Department of microbiology, Alborz Branch, Islamic Azad University, karaj, Iran
2- associate professor ,Department of Microbiology, School of Medicine, Iran University of Medical Sciences,Tehran, Iran
3- Assistant Professor of Microbiology, Department of microbiology, Alborz Branch, Islamic Azad University, karaj, Iran , e.babapour@kiau.ac.ir
Abstract:   (61 Views)
Aims. Pseudomonas aeruginosa is one of the major causes of nosocomial infections.  This study aimed to investigate the antibacterial susceptibility and the prevalence of virulence and resistance genes of P. aeruginosa isolated from patients in Tehran, Iran.
Materials and Methods. In this cross-sectional study, 70 P. aeruginosa isolates from burn infection and cystic fibrosis patients were collected from Shahid Motahari Hospital and Pediatric Medical Center, Tehran, Iran during 2017-2018. Antibacterial susceptibility against eleven antibiotics was determined based on disk diffusion method. Isolates were then screened for the presence of virulence and resistance genes by PCR.
Findings. The highest and lowest antibacterial resistance rates were against ampicillin and meropenem respectively. The oprI and oprL genes were present in all P. aeruginosa isolates. The prevalence of toxA,  exoS, exoU, exoT,and  exoY genes in P. aeruginosa isolated from a total of 18 CF patients was 66.6%, 66.6%, 22.2%, 72.2%, and 77.7%; and in isolates from a total of 52 burn patients was  84.7%, 100%, 28.8%, 73.07%, and 64.46%, respectively. , VEB, PER, TEM, SHV, and CTX-M  genes were found in 0.0%, 0.0%, 11.1%, 16.6%, and 5.5% P. aeruginosa isolated from CF patients; and in 0.0%, 1 1.9%, 50 96.1%, 88.4%, and 40.3%, P. aeruginosa isolated from burn patients, respectively.
Conclusion. Generally, selective pressure caused by extensive use of antibiotics can be conducive to the selection of MDR bacteria. Therefore, choosing suitable antibiotic based on precise antibiogram tests can prevent the increase of resistance in bacteria.

Received: 2018/12/12

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