Volume 9, Issue 1 (2023)
IEM 2023, 9(1): 55-62 |
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Mokhtari M, Mojtahedi A, Mahdieh N, Jafari A, Atrkar Roushan Z, Arya M J. Evaluation of the Relative Frequency of Carbapenemase Genes by Phenotypic and Genotypic Methods in Pseudomonas aeruginosa Isolates from Patients with Open Heart Surgery in Iran. IEM 2023; 9 (1) :55-62
URL: http://iem.modares.ac.ir/article-4-66610-en.html
URL: http://iem.modares.ac.ir/article-4-66610-en.html
Maryam Mokhtari1 
, Ali Mojtahedi * 2, Nejat Mahdieh3 , Alireza Jafari4 , Zahra Atrkar Roushan5 , Mohammad Javad Arya6
, Ali Mojtahedi * 2, Nejat Mahdieh3 , Alireza Jafari4 , Zahra Atrkar Roushan5 , Mohammad Javad Arya6
1- Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
2- Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran , mojtahedi.a@iums.ac.ir
3- Cardiogenetic Research Center, Rajaei Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
4- Urology Research Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
5- School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
6- Anatomical and clinical Pathologist, Fellowship of dermatopathology, Head of Sina Pathobiology Lab, Yazd, Iran.
2- Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran , mojtahedi.a@iums.ac.ir
3- Cardiogenetic Research Center, Rajaei Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
4- Urology Research Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
5- School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
6- Anatomical and clinical Pathologist, Fellowship of dermatopathology, Head of Sina Pathobiology Lab, Yazd, Iran.
Abstract: (1047 Views)
Backgrounds: Carbapenem resistance among Pseudomonas aeruginosa strains is alarming. This study aimed to investigate the relative frequency of carbapenem-resistant P. aeruginosa strains by phenotypic and genotypic methods.
Materials & Methods: The antibiotic susceptibility pattern of 60 P. aeruginosa isolates was determined by disk diffusion method (Kirby-Bauer). BD Phoenix automated microbiology system was used to identify carbapenem-resistant isolates, and the minimum inhibitory concentration (MIC) was determined using E-Test. In addition, mCIM (modified carbapenem inactivation method) phenotypic test was performed to evaluate carbapenem resistance genes in P. aeruginosa isolates. The prevalence of metallo-beta-lactamase (MβL) genes in carbapenem-resistant P. aeruginosa isolates was determined using conventional polymerase chain reaction (PCR).
Findings: The frequency of carbapenem-resistant P. aeruginosa isolates was 36% (22 of 60). The highest resistance was observed to imipenem and meropenem (36.6%), and the highest sensitivity was observed to amikacin (75%). All carbapenem-resistant P. aeruginosa isolates were confirmed by the BD Phoenix automated system (MIC> 8 µg/mL for imipenem and meropenem), E-test (MIC ˂32 µg/mL), and mCIM assay (the growth inhibition zone diameter was 6-8 mm). In carbapenem-resistant P. aeruginosa isolates, the frequency of blaVIM, blaIMP, and blaSPM genes was 9.1% (2 of 22), 4.5% (1 of 22), and 4.5% (1 of 22), respectively. BlaKPC and blaNDM genes were not found in any of the isolates.
Conclusion: Based on the present study results, all phenotypic tests used to identify carbapenemase-producing isolates had the same sensitivity (100%) and specificity (100%).
Materials & Methods: The antibiotic susceptibility pattern of 60 P. aeruginosa isolates was determined by disk diffusion method (Kirby-Bauer). BD Phoenix automated microbiology system was used to identify carbapenem-resistant isolates, and the minimum inhibitory concentration (MIC) was determined using E-Test. In addition, mCIM (modified carbapenem inactivation method) phenotypic test was performed to evaluate carbapenem resistance genes in P. aeruginosa isolates. The prevalence of metallo-beta-lactamase (MβL) genes in carbapenem-resistant P. aeruginosa isolates was determined using conventional polymerase chain reaction (PCR).
Findings: The frequency of carbapenem-resistant P. aeruginosa isolates was 36% (22 of 60). The highest resistance was observed to imipenem and meropenem (36.6%), and the highest sensitivity was observed to amikacin (75%). All carbapenem-resistant P. aeruginosa isolates were confirmed by the BD Phoenix automated system (MIC> 8 µg/mL for imipenem and meropenem), E-test (MIC ˂32 µg/mL), and mCIM assay (the growth inhibition zone diameter was 6-8 mm). In carbapenem-resistant P. aeruginosa isolates, the frequency of blaVIM, blaIMP, and blaSPM genes was 9.1% (2 of 22), 4.5% (1 of 22), and 4.5% (1 of 22), respectively. BlaKPC and blaNDM genes were not found in any of the isolates.
Conclusion: Based on the present study results, all phenotypic tests used to identify carbapenemase-producing isolates had the same sensitivity (100%) and specificity (100%).
Article Type: Original Research |
Subject:
Bacteriology
Received: 2023/01/6 | Accepted: 2023/02/21 | Published: 2023/03/10
Received: 2023/01/6 | Accepted: 2023/02/21 | Published: 2023/03/10
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