Volume 10, Issue 1 (2024)
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Amini R, Derakhshan S, Abedini M. Detection of Mycoplasma pneumoniae in Pediatric Patients with Community-Acquired Pneumonia in Western Iran. IEM 2024; 10 (1)
URL: http://iem.modares.ac.ir/article-4-72014-en.html
URL: http://iem.modares.ac.ir/article-4-72014-en.html
1- Kurdistan University of Medical Sciences
2- Kurdistan University of Medical Sciences,
3- Kurdistan University of Medical Sciences, , mabedn293@gmail.com
2- Kurdistan University of Medical Sciences,
3- Kurdistan University of Medical Sciences, , mabedn293@gmail.com
Abstract: (75 Views)
Aims: Mycoplasma pneumoniae strains are among the main causes of community-acquired pneumonia (CAP) in humans. Early detection of this microorganism is important to improve treatment efficiency. This study aimed to detect M. pneumoniae (MP)-specific immunoglobulin M (IgM) and MP DNA among pediatric patients with CAP during one week after admission.
Materials & Methods: From September 2019 to February 2020, 56 CAP patients aged 5 to 15 years were investigated for the presence of MP. Throat swabs for molecular detection of MP and blood samples for detection of cold agglutinins and MP-specific IgG and IgM antibodies were collected at admission. Blood and throat samples were taken again 6 days after admission. Macrolide resistance due to mutations in the 23S rRNA gene was also investigated.
Findings: MP-specific IgM was found in 19.6%, IgG in 16.1%, and cold agglutinins in 26.8% of CAP patients. The combination of IgM+IgG was not found. Tachypnea and the need for intensive care were more common in IgM-positive than in IgM-negative patients. Only four patients were positive for MP DNA, of whom two patients carried macrolide-resistant isolates. One isolate had an A2063G mutation and the other had an A2064C mutation.
Conclusion: To the best of our knowledge, there are no data on the epidemiology of MP in 5-15-year-old patients with CAP in Kurdistan, western Iran. The possibility of false-positive or -negative reactions and co-presence with other microorganisms could not be excluded.
Materials & Methods: From September 2019 to February 2020, 56 CAP patients aged 5 to 15 years were investigated for the presence of MP. Throat swabs for molecular detection of MP and blood samples for detection of cold agglutinins and MP-specific IgG and IgM antibodies were collected at admission. Blood and throat samples were taken again 6 days after admission. Macrolide resistance due to mutations in the 23S rRNA gene was also investigated.
Findings: MP-specific IgM was found in 19.6%, IgG in 16.1%, and cold agglutinins in 26.8% of CAP patients. The combination of IgM+IgG was not found. Tachypnea and the need for intensive care were more common in IgM-positive than in IgM-negative patients. Only four patients were positive for MP DNA, of whom two patients carried macrolide-resistant isolates. One isolate had an A2063G mutation and the other had an A2064C mutation.
Conclusion: To the best of our knowledge, there are no data on the epidemiology of MP in 5-15-year-old patients with CAP in Kurdistan, western Iran. The possibility of false-positive or -negative reactions and co-presence with other microorganisms could not be excluded.
Keywords: Respiratory tract infection, Mycoplasma pneumoniae, Serology, Molecular diagnostic techniques, Macrolides, Drug resistance
Article Type: Original Research |
Subject:
Bacteriology
Received: 2023/10/16 | Accepted: 2024/04/9 | Published: 2024/02/21
Received: 2023/10/16 | Accepted: 2024/04/9 | Published: 2024/02/21
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