Volume 4, Issue 2 (2018)
IEM 2018, 4(2): 67-72 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Shams S, Mehdipour A, Kermani S, Ghorbani H, Ragolia S. Anti-Biofilm Activity of Punica granatum, Ricinus communis, and Allium sativum Plant Extracts on Streptococcus mutans. IEM 2018; 4 (2) :67-72
URL: http://iem.modares.ac.ir/article-4-23374-en.html
URL: http://iem.modares.ac.ir/article-4-23374-en.html
1- Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran , sshams@muq.ac.ir
2- Pediatric Dentistry Department, Dental Faculty, Qom University of Medical Sciences, Qom, Iran
3- Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
4- Student Research Committee, Qom University of Medical Sciences, Qom, Iran
5- Molecular Medicine Department, University of Padova, Padova, Italy
2- Pediatric Dentistry Department, Dental Faculty, Qom University of Medical Sciences, Qom, Iran
3- Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
4- Student Research Committee, Qom University of Medical Sciences, Qom, Iran
5- Molecular Medicine Department, University of Padova, Padova, Italy
Abstract: (6136 Views)
Aims: Streptococcus mutans (S. mutans) is part of human oral cavity microbiome and is known to be responsible of dental caries. The aim of this study was to evaluate the inhibitory effects of Punica granatum, Ricinus communis, and Allium sativum extracts on biofilm formation caused by S. mutans.
Materials and Methods: In this experimental study, the biofilm formation was carried out by broth dilution method with glucose -supplemented Tryptic Soy Agar (TSB) in 96-well microtiter plates. Seven serial dilutions from the aqueous extracts of the Punica granatum, Ricinus communis, and Allium sativum were prepared. Then, a suspension of S. mutans was added to the wells. The anti-biofilm effects of the extracts and turbidity were measured by an ELISA reader apparatus at OD492nm. Experiments were completed in triplicate.
Findings: Ricinus communis was more active on S. mutans than other extracts. In comparison with others, the mean OD obtained in the presence of a concentration of 50mg of the plant extract (OD=0.083) was close to the negative control (OD=0.068). This plant was effective in higher concentrations (50, 25, 12.5 and 6.25mg/ml). Allium sativum extract has a moderate effect on S. mutans. The lowest activity belonged to Punica granatum extract.
Conclusion: The extract of Ricinus communis has strong anti-biofilm activity against Streptococcus mutans, when compared to other extracts, Allium sativum extract show moderate activity on the biofilm formation. Aqueous extract of Punica granatum peel isn’t very effective on S. mutans.
Materials and Methods: In this experimental study, the biofilm formation was carried out by broth dilution method with glucose -supplemented Tryptic Soy Agar (TSB) in 96-well microtiter plates. Seven serial dilutions from the aqueous extracts of the Punica granatum, Ricinus communis, and Allium sativum were prepared. Then, a suspension of S. mutans was added to the wells. The anti-biofilm effects of the extracts and turbidity were measured by an ELISA reader apparatus at OD492nm. Experiments were completed in triplicate.
Findings: Ricinus communis was more active on S. mutans than other extracts. In comparison with others, the mean OD obtained in the presence of a concentration of 50mg of the plant extract (OD=0.083) was close to the negative control (OD=0.068). This plant was effective in higher concentrations (50, 25, 12.5 and 6.25mg/ml). Allium sativum extract has a moderate effect on S. mutans. The lowest activity belonged to Punica granatum extract.
Conclusion: The extract of Ricinus communis has strong anti-biofilm activity against Streptococcus mutans, when compared to other extracts, Allium sativum extract show moderate activity on the biofilm formation. Aqueous extract of Punica granatum peel isn’t very effective on S. mutans.
Article Type: Original Research |
Subject:
Bacteriology
Received: 2018/03/5 | Accepted: 2018/04/25 | Published: 2018/06/20
Received: 2018/03/5 | Accepted: 2018/04/25 | Published: 2018/06/20
References
1. Biswas S, Biswas I. Role of VltAB, an ABC transporter complex, in viologen tolerance in Streptococcus mutans. Antimicrob Agents Chemother. 2011;55(4):1460-9. [Link]
2. Krzyściak W, Jurczak A, Kościelniak D, Bystrowska B, Skalniak A. The virulence of Streptococcus mutans and the ability to form biofilms. Eur J Clin Microbiol Infect Dis. 2014;33(4):499-515. [Link]
3. Kunze B, Reck M, Dötsch A, Lemme A, Schummer D, Irschik H, et al. Damage of Streptococcus mutans biofilms by carolacton, a secondary metabolite from the myxobacterium Sorangium cellulosum. BMC Microbiol. 2010;10:199. [Link]
4. Chandki R, Banthia P, Banthia R. Biofilms: A microbial home. J Indian Soc Periodontol. 2011;15(2):111-4. [Link]
5. Singh R, Sahore S, Kaur P, Rani A, Ray P. Penetration barrier contributes to bacterial biofilm-associated resistance against only select antibiotics, and exhibits genus-, strain- and antibiotic-specific differences. Pathog Dis. 2016;74(6).pii:ftw056. [Link]
6. Autio-Gold J. The role of chlorhexidine in caries prevention. Oper Dent. 2008;33(6):710-6. [Link]
7. Palombo EA. Traditional medicinal plant extracts and natural products with activity against oral bacteria: Potential application in the prevention and treatment of oral diseases. Evid Based Complement Altern Med. 2011;2011:680354. [Link]
8. Taur DJ, Patil RY. Some medicinal plants with antiasthmatic potential: A current status. Asian Pac J Trop Biomed. 2011;1(5):413-8. [Link]
9. Venkatadri B, Arunagirinathan N, Rameshkumar MR, Ramesh L, Dhanasezhian A, Agastian P. In vitro antibacterial activity of aqueous and ethanol extracts of Aristolochia indica and Toddalia asiatica against multidrug-resistant bacteria. Indian J Pharm Sci. 2015;77(6):788-91. [Link]
10. Yatsuda R, Rosalen PL, Cury JA, Murata RM, Rehder VL, Melo LV, et al. Effects of Mikania genus plants on growth and cell adherence of mutans streptococci. J Ethnopharmacol. 2005;97(2):183-9. [Link]
11. Sánchez E, Rivas Morales C, Castillo S, Leos-Rivas C, García-Becerra L, Ortiz Martínez DM. Antibacterial and antibiofilm activity of methanolic plant extracts against nosocomial microorganisms. Evid Based Complement Altern Med. 2016;2016:1572697. [Link]
12. Motamedi H, Darabpour E, Gholipour M, Seyyed Nejad SM. In vitro assay for the anti-Brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis. J Zhejiang Univ Sci B. 2010;11(7):506-11. [Link]
13. Fakhrieh Kashan Z, Delavari M, Arbabi M, Hooshyar H. Therapeutic effects of Iranian herbal extracts against Trichomonas vaginalis. Iran Biomed J. 2017;21(5):285-93. [Link]
14. Al-Zoreky NS. Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. Int J Food Microbiol. 2009;134(3):244-8. [Link]
15. Malviya S, Arvind, Jha A, Hettiarachchy N. Antioxidant and antibacterial potential of pomegranate peel extracts. J Food Sci Technol. 2014;51(12):4132-7. [Link]
16. Kuete V. Physical, hematological, and histopathological signs of toxicity induced by African medicinal plants. In: Kuete V. Toxicological survey of African medicinal plants. Amsterdam: Elsevier Science; 2014. pp. 635-57. [Link]
17. Naz R, Bano A. Antimicrobial potential of Ricinus communis leaf extracts in different solvents against pathogenic bacterial and fungal strains. Asian Pac J Trop Biomed. 2012;2(12):944-7. [Link]
18. Al-Mamun MA, Akter Z, Uddin MJ, Ferdaus KM, Hoque KM, Ferdousi Z, et al. Characterization and evaluation of antibacterial and antiproliferative activities of crude protein extracts isolated from the seed of Ricinus communis in Bangladesh. BMC Complement Altern Med. 2016;16:211. [Link]
19. Sivam GP, Lampe JW, Ulness B, Swanzy SR, Potter JD. Helicobacter pylor--in vitro susceptibility to garlic (Allium sativum) extract. Nutr Cancer. 1997;27(2):118-21. [Link]
20. Khan ZK, Katiyar R. Potent antifungal activity of garlic (Allium sativum) against experimental murine dissemenated cryptococcosis. Pharm Biol. 2000;38(2):87-100. [Link]
21. Karuppiah P, Rajaram S. Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogens. Asian Pac J Trop Biomed. 2012;2(8):597-601. [Link]
22. Leuter H, Koch H, Lawson L. Therapeutic effects and applications of garlic and its preparation. In: Koch HP, Lawson LD. Garlic: The science and therapeutic application of Allium sativum L. and related species. Philadelphia: Williams & Wilkins; 1996. pp. 135-212. [Link]
23. De Beer D, Srinivasan R, Stewart PS. Direct measurement of chlorine penetration into biofilms during disinfection. Appl Environ Microbiol. 1994;60(12):4339-44. [Link]
24. Singh R, Ray P, Das A, Sharma M. Penetration of antibiotics through Staphylococcus aureus and Staphylococcus epidermidis biofilms. J Antimicrob Chemother. 2010;65(9):1955-8. [Link]
25. Algburi A, Comito N, Kashtanov D, Dicks LM, Chikindas ML. Control of biofilm formation: Antibiotics and beyond. Appl Environ Microbiol. 2017;83(3).pii:e02508-16. [Link]
26. Marsh PD. Dental plaque as a biofilm and a microbial community - implications for health and disease. BMC Oral Health. 2006;6(Suppl 1):S14. [Link]
27. Saini R, Saini S, Sharma S. Biofilm: A dental microbial infection. J Nat Sci Biol Med. 2011;2(1):71-5. [Link]
28. Ahn SJ, Ahn SJ, Wen ZT, Brady LJ, Burne RA. Characteristics of biofilm formation by Streptococcus mutans in the presence of saliva. Infect Immun. 2008;76(9):4259-68. [Link]
29. Hashemi A, Shams S, Barati M, Samedani A. Antibacterial effects of methanolic extracts of Zataria multiflora, Myrtus communis and Peganum harmala on Pseudomonas aeruginosa producing ESBL. J Arak Univ Med Sci. 2011;14(4):104-12. [Link]
30. Hashemi A, Shams S, Kalantar D, Taherpour A, Barati M. Antibacterial effect of Methanolic extract of Camellia Sinensis L. on Pseudomonas aeruginosa strains producing β-lactamases. J Gorgan Univ Med Sci. 2012;14(1):136-42. [Link]
31. Yang XN, Khan I, Kang SC. Chemical composition, mechanism of antibacterial action and antioxidant activity of leaf essential oil of Forsythia koreana deciduous shrub. Asian Pac J Trop Med. 2015;8(9):694-700. [Link] [DOI:10.1016/j.apjtm.2015.07.031]
32. Ulloa-Urizar G, Aguilar-Luis MA, De Lama-Odría MDC, Camarena-Lizarzaburu J, Del Valle Mendoza J. Antibacterial activity of five Peruvian medicinal plants against Pseudomonas aeruginosa. Asian Pac J Trop Biomed. 2015;5(11):928-31. [Link]
33. Wassel MO, Khattab MA. Antibacterial activity against Streptococcus mutans and inhibition of bacterial induced enamel demineralization of propolis, miswak, and chitosan nanoparticles based dental varnishes. J Adv Res. 2017;8(4):387-92. [Link]
34. Choi HA, Cheong DE, Lim HD, Kim WH, Ham MH, Oh MH, et al. Antimicrobial and anti-biofilm activities of the methanol extracts of medicinal plants against dental pathogens Streptococcus mutans and Candida albicans. J Microbiol Biotechnol. 2017;27(7):1242-8. [Link]
35. Leite VM, Pinheiro JB, Pisani MX, Watanabe E, De Souza RF, Paranhos Hde F, et al. In vitro antimicrobial activity of an experimental dentifrice based on Ricinus communis. Braz Dent J. 2014;25(3):191-6. [Link]
36. Suurbaar J, Mosobil R, Donkor AM. Antibacterial and antifungal activities and phytochemical profile of leaf extract from different extractants of Ricinus communis against selected pathogens. BMC Res Notes. 2017;10(1):660. [Link] [DOI:10.1186/s13104-017-3001-2]
37. Viswanathan V, Phadatare AG, Mukne A. Antimycobacterial and antibacterial activity of Allium sativum bulbs. Indian J Pharm Sci. 2014;76(3):256-61. [Link]
38. Fani MM, Kohanteb J, Dayaghi M. Inhibitory activity of garlic (Allium sativum) extract on multidrug-resistant Streptococcus mutans. J Indian Soc Pedod Prev Dent. 2007;25(4):164-8. [Link] [DOI:10.4103/0970-4388.37011]
39. Naz S, Siddiqi R, Ahmad S, Rasool SA, Sayeed SA. Antibacterial activity directed isolation of compounds from Punica granatum. J Food Sci. 2007;72(9):M341-5. [Link] [DOI:10.1111/j.1750-3841.2007.00533.x]
40. Ferrazzano GF, Scioscia E, Sateriale D, Pastore G, Colicchio R, Pagliuca C, et al. In vitro antibacterial activity of pomegranate juice and peel extracts on cariogenic bacteria. Biomed Res Int. 2017;2017:2152749. [Link]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |