Volume 4, Issue 3 (2018)
IEM 2018, 4(3): 105-108 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Shams S, Vesali Jamshid Z, Shahbazi T, Hasani M, Shams E, Ragolia S. Comparison of Serum IgG and IgA Levels against Helicobacter Pylori in Patients with Gastrointestinal Symptoms. IEM 2018; 4 (3) :105-108
URL: http://iem.modares.ac.ir/article-4-26040-en.html
URL: http://iem.modares.ac.ir/article-4-26040-en.html
1- Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran , sshams@muq.ac.ir
2- Student Research Committee, Qom University of Medical Sciences, Qom, Iran
3- Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
4- Molecular Medicine Department, University of Padova, Padova, Italy
2- Student Research Committee, Qom University of Medical Sciences, Qom, Iran
3- Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
4- Molecular Medicine Department, University of Padova, Padova, Italy
Abstract: (6323 Views)
Aims: Helicobacter pylori is a pathogen that can be colonized in the stomach. Most laboratories only use IgG and not IgA antibody to diagnose infection. The aim of this study was to compare both IgG and IgA-antibodies level for the detection H. pylori.
Materials & Methods: The presence of IgG and IgA antibodies in the sera of the 517 patients suspected to H. pylori infection was evaluated by Enzyme-Linked Immunoadsordent Assays (ELISA) method.
Findings: The positive cases of infection on the basis of IgG and IgA titers were 68% and 27%, respectively. Also, 7% of the patients with IgG negative were IgA positive.
Conclusion: The comparison of antibody responses in our patients indicate that the sensitivity of IgA level is lower than IgG ELISA and both antibody titers must be evaluated for the identification of infection. In some cases, patients with IgG negative may have IgA positive assays; therefore, in the serological diagnostic process and without endoscopy, IgG results in association with IgA against H. pylori will be completed.
Materials & Methods: The presence of IgG and IgA antibodies in the sera of the 517 patients suspected to H. pylori infection was evaluated by Enzyme-Linked Immunoadsordent Assays (ELISA) method.
Findings: The positive cases of infection on the basis of IgG and IgA titers were 68% and 27%, respectively. Also, 7% of the patients with IgG negative were IgA positive.
Conclusion: The comparison of antibody responses in our patients indicate that the sensitivity of IgA level is lower than IgG ELISA and both antibody titers must be evaluated for the identification of infection. In some cases, patients with IgG negative may have IgA positive assays; therefore, in the serological diagnostic process and without endoscopy, IgG results in association with IgA against H. pylori will be completed.
Article Type: Original Research |
Subject:
Bacteriology
Received: 2018/06/12 | Accepted: 2018/06/12 | Published: 2018/09/22
Received: 2018/06/12 | Accepted: 2018/06/12 | Published: 2018/09/22
References
1. El Malti J, Mountassif D, Amarouch H. Antimicrobial activity of Elettaria cardamomum: Toxicity, biochemical and histological studies. Food Chem. 2007;104(4):1560-8. [Link] [DOI:10.1016/j.foodchem.2007.02.043]
2. World Health Organization. Campylobacter [Internet]. Geneva: World Health Organization; 2018 [cited 2018 Aug 15]. Available from: http://www.who.int/mediacentre/factsheets/fs255/en/. [Link]
3. Asaka M, Sepulveda AR, Sugiyama T, Graham DY. Gastric cancer. In: Mobley HLT, Mendz GL, Hazell SL, editors. Helicobacter pylori: Physiology and genetics. Washington DC: ASM Press; 2001. [Link] [DOI:10.1128/9781555818005.ch40]
4. Misiewicz JJ. Management of Helicobacter pylori-related disorders. Eur J Gastroenterol Hepatol. 1997;9 Suppl 1:S17-20. [Link] [DOI:10.1097/00042737-201204001-00005]
5. Abdollahi H, Shams S, Zahedi MJ, Darvish Moghadam S, Hayatbakhsh MM, Jafarzadeh A. IL-10, TNF-α and IFN-γ levels in serum and stomach mucosa of Helicobacter pylori-infected patients. Iran J Allergy Asthma Immunol. 2011;10(4):267-71. [Link]
6. Takeoka A, Tayama J, Yamasaki H, Kobayashi M, Ogawa S, Saigo T, et al. Impact of Helicobacter pylori immunoglobulin G levels and atrophic gastritis status on risk of metabolic syndrome. PLoS One. 2016;11(11):e0166588. [Link] [DOI:10.1371/journal.pone.0166588]
7. Maruyama M, Kamimura K, Hoshiyama A, Hoshiyama K, Hoshiyama M, Hoshiyama Y, et al. Effect of Helicobacter pylori eradication on elder cases: Observational study in community-based medicine. World J Clin Cases. 2017;5(12):412-8. [Link] [DOI:10.12998/wjcc.v5.i12.412]
8. Yokota K, Ayada K, Ishii E, Oguma K, Yamasaki R, Kobayashi K, et al. Gastric mucosal immunity induced by H. pylori infection. Nihon Rinsho. 2001;59(2):342-8. [Japanese] [Link]
9. Kosunen TU, Seppälä K, Sarna S, Sipponen P. Diagnostic value of decreasing IgG, IgA, and IgM antibody titres after eradication of Helicobacter pylori. Lancet. 1992;339(8798):893-5. [Link] [DOI:10.1016/0140-6736(92)90929-W]
10. Faller G, Ruff S, Reiche N, Hochberger J, Hahn EG, Kirchner T. Mucosal production of antigastric autoantibodies in Helicobacter pylori gastritis. Helicobacter. 2000;5(3):129-34. [Link] [DOI:10.1046/j.1523-5378.2000.00020.x]
11. Bhuiyan TR, Saha A, Lundgren A, Qadri F, Svennerholm AM. Immune responses to Helicobacter pylori infection in Bangladeshi children during their first two years of life and the association between maternal antibodies and onset of infection. J Infect Dis. 2010;202(11):1676-84. [Link] [DOI:10.1086/657085]
12. Locatelli A, Catapani WR, Gomes CR Jr, Silva CB, Waisberg J. Detection of anti-Helicobacter pylori antibodies in serum and duodenal fluid in peptic gastroduodenal disease. World J Gastroenterol. 2004;10(20):2997-3000. [Link] [DOI:10.3748/wjg.v10.i20.2997]
13. Zapatier JA, Gómez NA, Vargas PE, Maya SV. Evaluation of serology as a diagnostic method for Helicobacter pylori infection in the local population of Guayaquil. Acta Gastroenterologica Latinoamericana. 2007;37(2):104-9. [Spanish] [Link]
14. Alem M, Alem N, Cohen H, England T, Hamedi N, Moussazadeh M, et al. Diagnostic value of detection of IgM antibodies to Helicobacter pylori. Exp Mol Pathol. 2002;72(1):77-83. [Link] [DOI:10.1006/exmp.2001.2408]
15. Manojlovic N, Babic D, Filipovic-Ljeshovic I, Pilcevic D. Anti Helicobacter pylori IgG and IgA response in patients with gastric cancer and chronic gastritis. Hepatogastroenterology. 2008;55(82-83):807-13. [Link]
16. Pandya HB, Patel JS, Agravat HH, Ramdayal Singh NK. Non-invasive diagnosis of Helicobacter pylori: Evaluation of two enzyme immunoassays, testing serum IgG and IgA response in the Anand district of central Gujarat, India. J Clin Diagn Res. 2014;8(6):DC12-5. [Link] [DOI:10.7860/JCDR/2014/7578.4480]
17. Shin CM, Kim N, Lee HS, Lee HE, Lee SH, Park YS, et al. Validation of diagnostic tests for Helicobacter pylori with regard to grade of atrophic gastritis and/or intestinal metaplasia. Helicobacter. 2009;14(6):512-9. [Link] [DOI:10.1111/j.1523-5378.2009.00726.x]
18. Hung HH, Chen TS, Lin HC. Immunoglobulin G antibody against Helicobacter pylori is an accurate test for atrophic gastritis. J Chin Med Assoc. 2010;73(7):355-9. [Link] [DOI:10.1016/S1726-4901(10)70077-0]
19. George S, Mamani N, Lucero Y, Torres JP, Farfán M, Lagomarcino AJ, et al. Detection of Helicobacter pylori by real‐time PCR for 16s rRNA in stools of noninfected healthy children, using ELISA antigen stool test as the gold standard. Helicobacter. 2016;21(6):606-12. [Link] [DOI:10.1111/hel.12318]
20. She RC, Wilson AR, Litwin CM. Evaluation of Helicobacter pylori Immunoglobulin G (IgG), IgA, and IgM serologic testing compared to stool antigen testing. Clin Vaccine Immunol. 2009;16(8):1253-5. [Link] [DOI:10.1128/CVI.00149-09]
21. Kindermann A, Konstantopoulos N, Lehn N, Demmelmair H, Koletzko S. Evaluation of two commercial enzyme immunoassays, testing immunoglobulin G (IgG) and IgA responses, for diagnosis of Helicobacter pylori infection in children. J Clin Microbiol. 2001;39(10):3591-6. [Link] [DOI:10.1128/JCM.39.10.3591-3596.2001]
22. Urita Y, Hike K, Torii N, Kikuchi Y, Kurakata H, Kanda E, et al. Comparison of serum IgA and IgG antibodies for detecting Helicobacter pylori infection. Intern Med. 2004;43(7):548-52. [Link] [DOI:10.2169/internalmedicine.43.548]
23. Granberg C, Mansikka A, Lehtonen OP, Kujari H, Grönfors R, Nurmi H, et al. Diagnosis of Helicobacter pylori infection by using pyloriset EIA-G and EIA-A for detection of serum immunoglobulin G (IgG) and IgA antibodies. J Clin Microbiol. 1993;31(6):1450-3. [Link]
24. Aromaa A, Kosunen TU, Knekt P, Maatela J, Teppo L, Heinonen OP, et al. Circulating anti-Helicobacter pylori immunoglobulin A antibodies and low serum pepsinogen I level are associated with increased risk of gastric cancer. Am J Epidemiol. 1996;144(2):142-9. [Link] [DOI:10.1093/oxfordjournals.aje.a008901]
25. Atalay C, Atalay G, Altinok M. Serum Helicobacter pylori IgG and IgA levels in patients with gastric cancer. Neoplasma. 2003;50(3):185-90. [Link]
26. Jaskowski TD, Martins TB, Hill HR, Litwin CM. Immunoglobulin A antibodies to Helicobacter pylori. J Clin Microbiol. 1997;35(11):2999-3000. [Link]
27. Dinić M, Tasić G, Stanković-Dordević D, Otasević L, Tasić M, Karanikolic A. Serum anti-Helicobacter pylori IgA and IgG antibodies in asymptomatic children in Serbia. Scand J Infect Dis. 2007;39(4):303-7. [Link] [DOI:10.1080/00365540601034741]
28. Gościniak G. IgG and IgA antibodies in Helicobacter pylori infections. Zentralblatt für Bakteriologie. 1997;286(4):494-502. [Link] [DOI:10.1016/S0934-8840(97)80052-7]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |