Volume 6, Issue 1 (2020)                   IEM 2020, 6(1): 1-9 | Back to browse issues page


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Banihashem M, majidpour A, Boustanshenas M, Mazar-Atabaki S. Tetracycline Resistance Associated with tet Genes or Integrons among Enterobacter cloacae Strains Isolated from Patients with Urinary Tract Infections. IEM. 2020; 6 (1) :1-9
URL: http://iem.modares.ac.ir/article-4-39917-en.html
1- Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran. and Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran.
2- Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran. and Department of Infectious Diseases, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
3- Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran , mina_bustanshenas@yahoo.com
4- Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
Abstract:   (2300 Views)
Background: This study aimed to evaluate the prevalence of tet genes and Class I and 2 integrons in Enterobacter cloacae strains isolated from patients with urinary tract infections (UTIs).
Materials & Method: A total of 50 E. cloacae isolates were collected. Antimicrobial susceptibility pattern and tetracycline MIC were determined. The presence of tet genes (tetA, tetB, tetC, tetD) and Class 1 and 2 integrons and the content of Class 1 integron were determined.
Findings: Tetracycline MIC pattern classified 36 % of the E. cloacae isolates as resistant. The most common tet gene was tetC (22%), followed by tetD, tetA, and tetB.  Class 1 integron was detected in 64% of the isolates. Class 1 integron content analysis showed two variable gene cassettes (aadA1 and aadA5/dfrA17 genes). The frequency of aadA5/dfrA17 was 18.75%, which was more common than aadA1 gene (6.25%).
Conclusion: The most important genetic markers for tetracycline resistance in E. cloacae isolates were tetC and Class 1 integron. Harboring Class 1 integron and resistance to streptomycin and ciprofloxacin were significantly correlated.
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Article Type: Original Research | Subject: Bacteriology
Received: 2020/01/15 | Accepted: 2020/04/10 | Published: 2020/06/7

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