Volume 6, Issue 3 (2020)
IEM 2020, 6(3): 165-175 |
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Feliciano O, Ybalmea Y, Yglesias A, Diaz A, Llanes R, Gutierrez O. Synergic Effect of Curcuma longa L. Extract with Antimicrobials against Cuban Helicobacter pylori Isolates. IEM 2020; 6 (3) :165-175
URL: http://iem.modares.ac.ir/article-4-44472-en.html
URL: http://iem.modares.ac.ir/article-4-44472-en.html
Onelkis Feliciano * 
1, Yandy Ybalmea2 , Arianna Yglesias2 , Alexis Diaz2 , Rafael Llanes3 , Oderay Gutierrez3
1, Yandy Ybalmea2 , Arianna Yglesias2 , Alexis Diaz2 , Rafael Llanes3 , Oderay Gutierrez3
1- Research, Diagnostic and Reference Center. Pedro Kouri Tropical Medicine Institute. , onelkisfs@gmail.com
2- Department of Research, Laboratories of Biopharmaceuticals and Chemistries Productions (LABIOFAM)
3- Research, Diagnostic and Reference Center. Pedro Kouri Tropical Medicine Institute
2- Department of Research, Laboratories of Biopharmaceuticals and Chemistries Productions (LABIOFAM)
3- Research, Diagnostic and Reference Center. Pedro Kouri Tropical Medicine Institute
Abstract: (1697 Views)
Aims: Helicobacter pylori is a bacterium that colonizes the gastric mucosa and is the main cause of gastritis as well as ulcer and gastric cancer. Due to the clinical significance and international increase in H. pylori multidrug resistance, it is necessary to search for new strategies improving eradication rates. Natural compounds have been demonstrated to have antimicrobial effect and the ability to restore the efficacy of conventional drugs. The objective of this work was to evaluate the antimicrobial effect of the hydroalcoholic extract of Curcuma longa L. (Cu) against H. pylori isolates.
Materials & Methods: The minimum bactericidal concentration of the extract was determined by means of the MTT assay; also, the combination and dose reduction indices for levofloxacin (LVX), metronidazole (MET), and rifampicin (RIF) antimicrobial agents were determined by checkerboard format. Interaction analysis was performed using the CompuSyn program.
Findings: About 90% of H. pylori isolates studied (9/10) were sensitive to the hydroalcoholic extract. Synergism was observed in more than 50% of Cu-LVX, Cu-MET, and Cu-RIF combinations. Additionally, for different concentrations of the extract, reduction rates in antimicrobial agents were determined to be between 0.5 and 360 times.
Conclusions: The hydroalcoholic extract of turmeric showed a good potential to be used as an antimicrobial agent in the treatment of H. pylori infection, either alone or in combination with antibiotics used, suggesting the renewal of the effectiveness of conventional antimicrobials in reducing the phenomenon of antimicrobial resistance.
Materials & Methods: The minimum bactericidal concentration of the extract was determined by means of the MTT assay; also, the combination and dose reduction indices for levofloxacin (LVX), metronidazole (MET), and rifampicin (RIF) antimicrobial agents were determined by checkerboard format. Interaction analysis was performed using the CompuSyn program.
Findings: About 90% of H. pylori isolates studied (9/10) were sensitive to the hydroalcoholic extract. Synergism was observed in more than 50% of Cu-LVX, Cu-MET, and Cu-RIF combinations. Additionally, for different concentrations of the extract, reduction rates in antimicrobial agents were determined to be between 0.5 and 360 times.
Conclusions: The hydroalcoholic extract of turmeric showed a good potential to be used as an antimicrobial agent in the treatment of H. pylori infection, either alone or in combination with antibiotics used, suggesting the renewal of the effectiveness of conventional antimicrobials in reducing the phenomenon of antimicrobial resistance.
Article Type: Original Research |
Subject:
Bacteriology
Received: 2020/07/15 | Accepted: 2020/09/10 | Published: 2020/08/30
Received: 2020/07/15 | Accepted: 2020/09/10 | Published: 2020/08/30
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