Volume 6, Issue 4 (2020)                   IEM 2020, 6(4): 285-291 | Back to browse issues page


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Alimehr S, Shams-Ghahfarokhi M, Razzaghi Abyaneh M. Antifungal Activity of Aureobasidin A in Combination with Fluconazole against Fluconazole-Resistant Candida albicans . IEM. 2020; 6 (4) :285-291
URL: http://iem.modares.ac.ir/article-4-46415-en.html
1- Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14115-331, Iran
2- Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14115-331, Iran , shamsm@modares.ac.ir
3- Department of Mycology, Pasteur Institute of Iran, Tehran 13164, Iran
Abstract:   (433 Views)
Background: Aureobasidin A is known as a cyclic depsipeptide antibiotic with toxic effects against yeasts such as Candida spp at low concentration. Combination therapy is used as a conventional treatment for fungal infections, especially drug-resistant cases. The current study aimed to investigate the combined effects of fluconazole and Aureobasidin A on fluconazole-resistant C. albicans isolates using broth microdilution method.
Materials & Methods: Antifungal activity of Aureobasidin A (AbA) compared to fluconazole against C. albicans ATCC 76615 strain was determined using the standardized broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI, document M27-Ed4) guidelines. The checkerboard method was used to test the combined effects of Aureobasidin A and fluconazole. The synergy, indifference, and antagonism were defined based on the fractional inhibitory concentration values below 0.5, 0.5-4, and more than 4 μg/mL, respectively.
Findings: MIC50 and MIC90 evaluations of Aureobasidin A and fluconazole were done at concentrations of 0.25-2 and 32-64 μg/mL against C. glabrata isolates, respectively. The synergy between fluconazole and Aureobasidin A was observed against Candida isolate. A reduced MIC was demonstrated against C. albicans isolate when fluconazole was combined with Aureobasidin A at 4 to 0.12 μg/mL concentrations.
Conclusion: The present study findings revealed that Aureobasidin A combined with fluconazole exhibited potent inhibitory effects against fluconazole-resistant C. albicans isolates. Further studies is recommended to investigate the synergistic effects of Aureobasidin A and other antifungal drugs.

 
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Article Type: Original Research | Subject: Mycology
Received: 2020/09/29 | Accepted: 2020/12/17 | Published: 2020/11/20

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