Volume 8, Issue 2 (2022)                   IEM 2022, 8(2): 0-0 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mardani S, Fozouni L, Najafpour G. Zinc Oxide Nanoparticles: A Promising Solution for Controlling the Growth of Gentamicin-Resistant Uropathogenic Escherichia coli. IEM. 2022; 8 (2)
URL: http://iem.modares.ac.ir/article-4-60149-en.html
1- Chemical Engineering Group, Mazandaran Institute of Technology, Babol, Iran
2- Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran , lili_kia@yahoo.com
3- Biotechnology Research Laboratory, Faculty of Chemical Engineering, Noshirvani University of Technology, Babol, Iran
Abstract:   (65 Views)
Backgrounds: Uropathogenic Escherichia coli is one of the most important etiological agents of UTI. The aim of this study was to investigate the antibacterial effects of zinc oxide nanoparticles (ZnONPs) on aminoglycoside-resistant E. coli isolates from patients with UTI.
Materials & Methods: After identifying E. coli strains in 100 out of 250 urine samples, antibiotic susceptibility was evaluated against six antibiotic classes (with emphasis on aminoglycosides) by disk diffusion method according to CLSI-2020 guidelines. The presence of aac (6')-Ie-aph (2'') gene in isolates was investigated by PCR. Antibacterial properties and minimum inhibitory concentration (MIC) of zinc oxide nanoparticles were evaluated by agar well diffusion and broth microdilution assays, respectively.
Findings: Among 100 E. coli isolates, the highest and lowest antibiotic resistance rates were observed against tetracycline (70%) and ofloxacin (10%), respectively. Of 30 gentamicin-resistant E. coli isolates, 17 (56.5%) isolates harbored the aac (6')-Ie-aph (2'') gene. In agar well diffusion assay, 22 (74%) gentamicin-resistant isolates were eliminated by zinc oxide nanoparticles at a concentration of 150 mg/L, while ZnONPs at 300 mg/L could eliminate all gentamicin-resistant isolates. Furthermore, ZnONPs could inhibit all bacteria at a concentration of 200 μg/mL (MIC90 ≥ 100).
Conclusion: Spread of the aac(6')-Ie-aph(2'') gene could increase gentamicin resistance among E. coli strains causing UTI. Given the favorable antibacterial effects of zinc oxide nanoparticles in vitro, the clinical application of these nanoparticles in the treatment of UTIs caused by multidrug-resistant E. coli could be investigated in future studies.
Article Type: Original Research | Subject: Bacteriology
Received: 2022/03/8 | Accepted: 2022/05/26 | Published: 2022/06/30

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.