Volume 9, Issue 2 (2023)
IEM 2023, 9(2): 149-155 |
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Anand S, Mohanam L, Shanmugam P. Screening of Influenza Virus A and B among COVID-19 Patients in a Tertiary Care Hospital, Southern India. IEM 2023; 9 (2) :149-155
URL: http://iem.modares.ac.ir/article-4-69080-en.html
URL: http://iem.modares.ac.ir/article-4-69080-en.html
1- Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai -603103, India
2- Department of Microbiology Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai -603103, India ,lavanyam@care.edu.in
3- Department of MicrobiologyChettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai -603103, India
2- Department of Microbiology Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai -603103, India ,
3- Department of MicrobiologyChettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai -603103, India
Abstract: (582 Views)
Aims: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and influenza virus are quite distant viruses, but they share significant similarities such as mode of transmission and clinical manifestations. No specific clinical signs reliably distinguish early influenza sickness from the disease caused by SARS-CoV-2 (COVID-19); therefore, it will be critical in clinical practice to determine the viral etiology. The present study aimed to screen for influenza virus A and B among COVID-19 patients by quantitative reverse transcription polymerase chain reaction (RT-qPCR).
Materials & Methods: A total of 100 nasal swabs from COVID-19 patients were collected in viral transport medium (VTM) during June to July 2022. RNA extraction was done using QIagen RNA extraction kit, and then RT-qPCR was performed using HELINI swine flu (H1N1) kit.
Findings: The average age of the study participants was 31 years, and 13 patients were hospitalized due to the COVID infection. Hypertension, diabetes, and chronic lung, heart and kidney diseases were identified as comorbidities. It was found that none of the tested samples were positive for influenza A and B.
Conclusion: Although none of the patients were positive for influenza, the importance of co-infection could not be ignored. Screening of a large number of samples is needed during the seasonal period.
Materials & Methods: A total of 100 nasal swabs from COVID-19 patients were collected in viral transport medium (VTM) during June to July 2022. RNA extraction was done using QIagen RNA extraction kit, and then RT-qPCR was performed using HELINI swine flu (H1N1) kit.
Findings: The average age of the study participants was 31 years, and 13 patients were hospitalized due to the COVID infection. Hypertension, diabetes, and chronic lung, heart and kidney diseases were identified as comorbidities. It was found that none of the tested samples were positive for influenza A and B.
Conclusion: Although none of the patients were positive for influenza, the importance of co-infection could not be ignored. Screening of a large number of samples is needed during the seasonal period.
Keywords: Acute respiratory distress syndrome (ARDS), Co-infection, COVID-19, Influenza A and B, RT-qPCR
Article Type: Original Research |
Subject:
Virology
Received: 2023/05/12 | Accepted: 2023/06/19 | Published: 2023/08/19
Received: 2023/05/12 | Accepted: 2023/06/19 | Published: 2023/08/19
References
1. 1.Yuki K, Fujiogi M, Koutsogiannaki S. COVID-19 pathophysiology: A review. J. Clin Immunol. 2020;215:108427. [DOI:10.1016/j.clim.2020.108427] [PMID] []
2. Dey A, Vaishak K, Deka D, Radhakrishnan AK, Paul S, Shanmugam P, et al. Epigenetic perspectives associated with COVID-19 infection and related cytokine storm: An updated review. Infection. 2023;12:1-16. [DOI:10.1007/s15010-023-02017-8] [PMID] []
3. Awan UA, Zahoor S, Rehman K, Khattak AA, Ahmed H, Aftab N, et al. COVID‐19 and influenza H1N1: A dangerous combination for Pakistan in the upcoming winter season. J Med Virol. 2021;93(4):1875-7. [DOI:10.1002/jmv.26687] [PMID] []
4. Hu B, Guo H, Zhou P, Shi ZL. Characteristics of SARS-CoV-2 and COVID-19. Nat Rev Microbiol. 2021;19(3):141-54. [DOI:10.1038/s41579-020-00459-7] [PMID] []
5. Low D. Reducing antibiotic use in influenza: Challenges and rewards. Clin Microbiol Infect. 2008;14(4):298-306. [DOI:10.1111/j.1469-0691.2007.01910.x] [PMID]
6. World Health Organization. Laboratory testing strategy recommendations for COVID-19: Interim guidance. Geneva: World Health Organization; 2020.
7. Solomon DA, Sherman AC, Kanjilal S. Influenza in the COVID-19 era. Jama. 2020;324(13):1342-3. [DOI:10.1001/jama.2020.14661] [PMID]
8. Konala VM, Adapa S, Gayam V, Naramala S, Daggubati SR, Kammari CB, et al. Co-infection with Influenza A and COVID-19. Eur J Case Rep Intern Med. 2020;7(5):001656. [DOI:10.12890/2020_001656] [PMID] []
9. Cheng Y, Ma J, Wang H, Wang X, Hu Z, Li H, et al. Co‐infection of influenza A virus and SARS‐CoV‐2: A retrospective cohort study. J Med Virol. 2021;93(5):2947-54. [DOI:10.1002/jmv.26817] [PMID] []
10. Azekawa S, Namkoong H, Mitamura K, Kawaoka Y, Saito F. Co-infection with SARS-CoV-2 and influenza A virus. IDCases. 2020;20:e00775. [DOI:10.1016/j.idcr.2020.e00775] [PMID] []
11. Alosaimi B, Naeem A, Hamed ME, Alkadi HS, Alanazi T, Al Rehily SS, et al. Influenza co-infection associated with severity and mortality in COVID-19 patients. Virol J. 2021;18(1):1-9. [DOI:10.1186/s12985-021-01594-0] [PMID] []
12. Dadashi M, Khaleghnejad S, Abedi Elkhichi P, Goudarzi M, Goudarzi H, Taghavi A, et al. COVID-19 and influenza co-infection: A systematic review and meta-analysis. Front Med. 2021;8:681469. [DOI:10.3389/fmed.2021.681469] [PMID] []
13. Agarwal A, Sharma A, Agarwal M, Jakhar R. The clinical characteristics of coinfection of COVID-19 and influenza A viruses-a case series. J Assoc Physicians India. 2021;69(5):33-7.
14. Agarwal A, Agarwal M, Sharma A, Jakhar R. Impact of influenza A co-infection with COVID-19. Int J Tuberc Lung Dis. 2021;25(5):413-5. [DOI:10.5588/ijtld.21.0086] [PMID]
15. Singh B, Kaur P, Reid RJ, Shamoon F, Bikkina M. COVID-19 and influenza co-infection: Report of three cases. Cureus. 2020;12(8):e9852. [DOI:10.7759/cureus.9852]
16. Munivenkatappa A, Yadav PD, Swetha K, Jayaswamy M, Nyayanit DA, Sahay RR, et al. SARS-CoV-2 & influenza A virus co-infection in an elderly patient with pneumonia. Indian J Med Res. 2021;153(1-2):190-5. [DOI:10.4103/ijmr.IJMR_2711_20] [PMID] []
17. Aggarwal N, Potdar V, Vijay N, Mukhopadhyay L, Borkakoty B, Manjusree S, et al. SARS-CoV-2 and influenza virus co-infection cases identified through ILI/SARI sentinel surveillance: A pan-India report. Viruses. 2022;14(3):627. [DOI:10.3390/v14030627] [PMID] []
18. Ozaras R, Cirpin R, Duran A, Duman H, Arslan O, Bakcan Y, et al. Influenza and COVID‐19 coinfection: Report of six cases and review of the literature. J Med Virol. 2020;92(11):2657-65. [DOI:10.1002/jmv.26125] [PMID]
19. Olsen SJ, Azziz-Baumgartner E, Budd AP, Brammer L, Sullivan S, Pineda RF, et al. Decreased influenza activity during the covid-19 pandemic-United States, Australia, Chile, and South Africa, 2020. Am J Transplant. 2020;20(12):3681-5. [DOI:10.1111/ajt.16381] [PMID] []
20. Chotpitayasunondh T, Fischer TK, Heraud JM, Hurt AC, Monto AS, Osterhaus A, et al. Influenza and COVID‐19: What does co‐existence mean? Influenza Other Respir Viruses. 2021;15(3):407-12. [DOI:10.1111/irv.12824] [PMID] []
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