Volume 9, Issue 3 (2023)
IEM 2023, 9(3): 219-228 |
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Ghafari S, Namakin K, khooban A R, askari P, Yousefi M, Ziaee M. Comparison of Multiplex Quantitative Real-Time PCR and Culture Methods for the Diagnosis of Bacterial Meningitis in Patients with Suspected Meningitis. IEM 2023; 9 (3) :219-228
URL: http://iem.modares.ac.ir/article-4-71306-en.html
URL: http://iem.modares.ac.ir/article-4-71306-en.html
Shokouh Ghafari1 
, Kokab Namakin2 , Ali Reza Khooban3 , Parvin Askari3 
, Masoud Yousefi3 , Masood Ziaee * 4
, Kokab Namakin2 , Ali Reza Khooban3 , Parvin Askari3 , Masoud Yousefi3 , Masood Ziaee * 4
1- Cellular and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran. Department of Microbiology and Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
2- Cardiovascular Diseases Research Center, Department of Pediatrics, Birjand University of Medical Sciences, Birjand, Iran Birjand University of Medical Sciences, Birjand, Iran
3- Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
4- Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran ,formaluse1368@gmail.com
2- Cardiovascular Diseases Research Center, Department of Pediatrics, Birjand University of Medical Sciences, Birjand, Iran Birjand University of Medical Sciences, Birjand, Iran
3- Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
4- Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran ,
Abstract: (1151 Views)
Background: This study aimed to compare the diagnostic efficacy of standard culture method with multiplex quantitative real-time polymerase chain reaction (qPCR) in examining cerebrospinal fluid (CSF) samples collected from patients with suspected meningitis.
Materials & Methods: A retrospective evaluation was conducted on 166 patients with suspected meningitis, who were treated in Vali-Asr hospital in Birjand, Iran between 2011 and 2020. Diagnosis of bacterial meningitis was based on CSF culture and multiplex qPCR results.
Findings: Among 166 patients, conventional methods identified causative pathogens in only 10.3% of cases, while multiplex qPCR detected pathogens in eight out of 25 culture-negative cases as well. The most common pathogens identified were enterovirus, Epstein-Barr virus, herpes simplex, Haemophilus influenzae, and Streptococcus pneumoniae.
Conclusion: Multiplex qPCR appears to be a more effective method than conventional culture in identifying bacterial and viral pathogens that most commonly cause meningitis. The incorporation of qPCR as a routine diagnostic method for meningitis in clinical practice could significantly enhance clinical decision-making and patient care.
Materials & Methods: A retrospective evaluation was conducted on 166 patients with suspected meningitis, who were treated in Vali-Asr hospital in Birjand, Iran between 2011 and 2020. Diagnosis of bacterial meningitis was based on CSF culture and multiplex qPCR results.
Findings: Among 166 patients, conventional methods identified causative pathogens in only 10.3% of cases, while multiplex qPCR detected pathogens in eight out of 25 culture-negative cases as well. The most common pathogens identified were enterovirus, Epstein-Barr virus, herpes simplex, Haemophilus influenzae, and Streptococcus pneumoniae.
Conclusion: Multiplex qPCR appears to be a more effective method than conventional culture in identifying bacterial and viral pathogens that most commonly cause meningitis. The incorporation of qPCR as a routine diagnostic method for meningitis in clinical practice could significantly enhance clinical decision-making and patient care.
Article Type: Original Research |
Subject:
Bacteriology
Received: 2023/08/29 | Accepted: 2023/10/18 | Published: 2023/10/18
Received: 2023/08/29 | Accepted: 2023/10/18 | Published: 2023/10/18
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