Volume 9, Issue 4 (2023)
IEM 2023, 9(4): 277-286 |
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Sadoogh Abbasian S, Sadoogh Abbasian A, Ghaznavi-Rad E, Zarei-Mehrvarz E, Sadelaji S, Abtahi H. LL37-rIb-AMP4 Hybrid Peptide as a Therapy for Systematic Infections of Acinetobacter baumannii, Pseudomonas aeruginosa, Vancomycin-resistant Enterococcus (VRE), and Methicillin-resistant Staphylococcus aureus (MRSA) cells in a mouse model.. IEM 2023; 9 (4) :277-286
URL: http://iem.modares.ac.ir/article-4-71409-en.html
URL: http://iem.modares.ac.ir/article-4-71409-en.html
Shabnam Sadoogh Abbasian1 
, Ali Sadoogh Abbasian2 , Ehsanollah Ghaznavi-Rad1 , Ehsan Zarei-Mehrvarz1 , Samira Sadelaji1 , Hamid Abtahi * 3
, Ali Sadoogh Abbasian2 , Ehsanollah Ghaznavi-Rad1 , Ehsan Zarei-Mehrvarz1 , Samira Sadelaji1 , Hamid Abtahi * 3
1- Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran.
2- Department of Internal Medicine, School of Medicine, Amiralmomenin Hospital, Arak University of Medical Sciences, Arak, Iran.
3- Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran. ,Abtahi@acad.mu.ac.ir
2- Department of Internal Medicine, School of Medicine, Amiralmomenin Hospital, Arak University of Medical Sciences, Arak, Iran.
3- Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran. ,
Abstract: (1331 Views)
Aims: Antimicrobial peptides (AMPs) are beneficial compounds that could be used as a new and effective method to suppress microbes. Both Ib-AMP4 and LL37 are antimicrobial peptides with a wide range of antimicrobial activities. This research aimed to evaluate the antibacterial potential of LL37-rIb-AMP4 hybrid protein as an antimicrobial agent against pathogenic bacteria. Therefore, its antibacterial effects against Acinetobacter baumannii, Pseudomonas aeruginosa, vancomycin-resistant Enterococcus (VRE), and methicillin-resistant Staphylococcus aureus (MRSA) were investigated in vivo and in vitro.
Materials & Methods: In this study, antimicrobial peptides rIb-AMP4, LL37, and LL37-rIb-AMP4 were expressed, purified, and refolded, and their synergistic and antibacterial effects in combination with each other (LL37+rIb-AMP4) and as fusion proteins (LL37-rIb-AMP4) were tested against A. baumannii, P. aeruginosa, VRE, and MRSA cells in vitro (MIC, time kill, and SEM) and against P. aeruginosa and VRE cells in vivo.
Findings: LL37-rIb-AMP4 Protein with molecular weight= 28 KD was correctly produced and purified. Despite the lack of synergistic effects between LL37 and rIb-AMP4 peptides in vitro, the stability test results showed higher stability for LL37-rIb-AMP4 hybrid protein.
The findings of in vivo tests confirmed that all infected mice were improved with LL37-rIb-AMP4 and no signs of bacteria were observed in their blood and spleen samples. Also, these results confirmed the stability and higher activity of LL37-rIb-AMP4 than the single form of these proteins.
Conclusion: Considering the antimicrobial potential of the produced proteins, it seems that the recombinant LL37-rIb-AMP4 protein could be considered and used as a stable and active antimicrobial drug in future studies.
Findings: LL37-rIb-AMP4 Protein with molecular weight= 28 KD was correctly produced and purified. Despite the lack of synergistic effects between LL37 and rIb-AMP4 peptides in vitro, the stability test results showed higher stability for LL37-rIb-AMP4 hybrid protein.
The findings of in vivo tests confirmed that all infected mice were improved with LL37-rIb-AMP4 and no signs of bacteria were observed in their blood and spleen samples. Also, these results confirmed the stability and higher activity of LL37-rIb-AMP4 than the single form of these proteins.
Conclusion: Considering the antimicrobial potential of the produced proteins, it seems that the recombinant LL37-rIb-AMP4 protein could be considered and used as a stable and active antimicrobial drug in future studies.
Article Type: Original Research |
Subject:
Bacteriology
Received: 2023/09/5 | Accepted: 2024/01/30 | Published: 2024/02/5
Received: 2023/09/5 | Accepted: 2024/01/30 | Published: 2024/02/5
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