Volume 11, Issue 1 (2025)
IEM 2025, 11(1): 63-75 |
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Fazeli S, Rafiee F, Ferdousi A. Biosynthesis of Copper Nanoparticles using Artemisia biennis Willd Plant Extract for Antibacterial and Anti-Biofilm Activities. IEM 2025; 11 (1) :63-75
URL: http://iem.modares.ac.ir/article-4-75809-en.html
URL: http://iem.modares.ac.ir/article-4-75809-en.html
1- Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
2- Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran ,Rafiee_ar@qodsiau.ac.ir
2- Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran ,
Abstract: (302 Views)
Background: This research aimed to assess the antibacterial and anti-biofilm properties of copper nanoparticles (CuNPs) produced using Artemisia biennis Willd through an eco-friendly approach, targeting four pathogenic bacteria.
Materials & Methods: A. biennis Willd extract with unit numbers “15.62-125” was prepared through maceration, drying, and powdering. Particle size distribution (PSD), dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR) tests were used to characterize the synthesized CuNPs. Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and sub-minimum inhibitory concentrations (sub-MICs) were determined to investigate the antibacterial and anti-biofilm activities of CuNPs against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 13883.
Findings: CuNPs synthesized using A. biennis Willd extract exhibited a brown color change with particle sizes mainly 30-40 nm by PSD. DLS indicated uniform distribution and hydrodynamic synthesis of particles with a zeta potential of -37.8. XRD and FTIR confirmed copper nanoparticle biosynthesis. The MICs of CuNPs were 15.62-62.5 μg/mL, with S. aureus and K. pneumonia revealing the highest and lowest antimicrobial drug resistance, respectively. This trend was repeated for MBCs and sub-MICs, ranging from 15.62-125 and 7.8-31.25 μg/mL, respectively. Bacterial strains were unable to form biofilms at sub-MICs. The anti-biofilm effects of CuNPs were more significant on Gram-negative bacteria.
Conclusion: CuNPs synthesized using A. biennis Willd extract by a green method show promising anti-biofilm and antibacterial characteristics against bacteria, suggesting their potential for treating bacterial infections.
Materials & Methods: A. biennis Willd extract with unit numbers “15.62-125” was prepared through maceration, drying, and powdering. Particle size distribution (PSD), dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR) tests were used to characterize the synthesized CuNPs. Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and sub-minimum inhibitory concentrations (sub-MICs) were determined to investigate the antibacterial and anti-biofilm activities of CuNPs against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 13883.
Findings: CuNPs synthesized using A. biennis Willd extract exhibited a brown color change with particle sizes mainly 30-40 nm by PSD. DLS indicated uniform distribution and hydrodynamic synthesis of particles with a zeta potential of -37.8. XRD and FTIR confirmed copper nanoparticle biosynthesis. The MICs of CuNPs were 15.62-62.5 μg/mL, with S. aureus and K. pneumonia revealing the highest and lowest antimicrobial drug resistance, respectively. This trend was repeated for MBCs and sub-MICs, ranging from 15.62-125 and 7.8-31.25 μg/mL, respectively. Bacterial strains were unable to form biofilms at sub-MICs. The anti-biofilm effects of CuNPs were more significant on Gram-negative bacteria.
Conclusion: CuNPs synthesized using A. biennis Willd extract by a green method show promising anti-biofilm and antibacterial characteristics against bacteria, suggesting their potential for treating bacterial infections.
Keywords: Artemisia biennis Willd, Copper nanoparticles, Green synthesis, Antimicrobial drug resistance, Anti-biofilm
Article Type: Original Research |
Subject:
Bacteriology
Received: 2024/06/25 | Accepted: 2024/12/16 | Published: 2025/02/22
Received: 2024/06/25 | Accepted: 2024/12/16 | Published: 2025/02/22
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